Tramtrack acts during late pupal development to direct ant caste identity.

A key question in the rising field of neuroepigenetics is how behavioral plasticity is established and maintained in the developing CNS of multicellular organisms. Behavior is controlled through systemic changes in hormonal signaling, cell-specific regulation of gene expression, and changes in neuro...

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Autores principales:	Karl M Glastad, Linyang Ju, Shelley L Berger
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/091c4f39037a43fabe0f5ac2ad203492
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spelling	oai:doaj.org-article:091c4f39037a43fabe0f5ac2ad2034922021-12-02T20:03:19ZTramtrack acts during late pupal development to direct ant caste identity.1553-73901553-740410.1371/journal.pgen.1009801https://doaj.org/article/091c4f39037a43fabe0f5ac2ad2034922021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009801https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404A key question in the rising field of neuroepigenetics is how behavioral plasticity is established and maintained in the developing CNS of multicellular organisms. Behavior is controlled through systemic changes in hormonal signaling, cell-specific regulation of gene expression, and changes in neuronal connections in the nervous system, however the link between these pathways is unclear. In the ant Camponotus floridanus, the epigenetic corepressor CoREST is a central player in experimentally-induced reprogramming of caste-specific behavior, from soldier (Major worker) to forager (Minor worker). Here, we show this pathway is engaged naturally on a large genomic scale during late pupal development targeting multiple genes differentially expressed between castes, and central to this mechanism is the protein tramtrack (ttk), a DNA binding partner of CoREST. Caste-specific differences in DNA binding of ttk co-binding with CoREST correlate with caste-biased gene expression both in the late pupal stage and immediately after eclosion. However, we find a unique set of exclusive Minor-bound genes that show ttk pre-binding in the late pupal stage preceding CoREST binding, followed by caste-specific gene repression on the first day of eclosion. In addition, we show that ttk binding correlates with neurogenic Notch signaling, and that specific ttk binding between castes is enriched for regulatory sites associated with hormonal function. Overall our findings elucidate a pathway of transcription factor binding leading to a repressive epigenetic axis that lies at the crux of development and hormonal signaling to define worker caste identity in C. floridanus.Karl M GlastadLinyang JuShelley L BergerPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 9, p e1009801 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Karl M Glastad Linyang Ju Shelley L Berger Tramtrack acts during late pupal development to direct ant caste identity.
description	A key question in the rising field of neuroepigenetics is how behavioral plasticity is established and maintained in the developing CNS of multicellular organisms. Behavior is controlled through systemic changes in hormonal signaling, cell-specific regulation of gene expression, and changes in neuronal connections in the nervous system, however the link between these pathways is unclear. In the ant Camponotus floridanus, the epigenetic corepressor CoREST is a central player in experimentally-induced reprogramming of caste-specific behavior, from soldier (Major worker) to forager (Minor worker). Here, we show this pathway is engaged naturally on a large genomic scale during late pupal development targeting multiple genes differentially expressed between castes, and central to this mechanism is the protein tramtrack (ttk), a DNA binding partner of CoREST. Caste-specific differences in DNA binding of ttk co-binding with CoREST correlate with caste-biased gene expression both in the late pupal stage and immediately after eclosion. However, we find a unique set of exclusive Minor-bound genes that show ttk pre-binding in the late pupal stage preceding CoREST binding, followed by caste-specific gene repression on the first day of eclosion. In addition, we show that ttk binding correlates with neurogenic Notch signaling, and that specific ttk binding between castes is enriched for regulatory sites associated with hormonal function. Overall our findings elucidate a pathway of transcription factor binding leading to a repressive epigenetic axis that lies at the crux of development and hormonal signaling to define worker caste identity in C. floridanus.
format	article
author	Karl M Glastad Linyang Ju Shelley L Berger
author_facet	Karl M Glastad Linyang Ju Shelley L Berger
author_sort	Karl M Glastad
title	Tramtrack acts during late pupal development to direct ant caste identity.
title_short	Tramtrack acts during late pupal development to direct ant caste identity.
title_full	Tramtrack acts during late pupal development to direct ant caste identity.
title_fullStr	Tramtrack acts during late pupal development to direct ant caste identity.
title_full_unstemmed	Tramtrack acts during late pupal development to direct ant caste identity.
title_sort	tramtrack acts during late pupal development to direct ant caste identity.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/091c4f39037a43fabe0f5ac2ad203492
work_keys_str_mv	AT karlmglastad tramtrackactsduringlatepupaldevelopmenttodirectantcasteidentity AT linyangju tramtrackactsduringlatepupaldevelopmenttodirectantcasteidentity AT shelleylberger tramtrackactsduringlatepupaldevelopmenttodirectantcasteidentity
_version_	1718375642864549888

Tramtrack acts during late pupal development to direct ant caste identity.

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