Effects of Two Polysaccharides From Traditional Chinese Medicines on Rat Immune Function

Abstract: The aim is to study the immune function effect of two polysaccharides extracted from traditional Chinese herbs on rats. Ultrasonic-assisted extraction was used to extract the polysaccharide from traditional Chinese medicines. MTT assay was used to determine the effects of two polysaccharid...

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Autores principales:	Yu Lei Wang, Xiang Hua Shu, Xue Zhang, Yong Bo Liu, Ya Jing Zhang, Tao Lv, Xin Huang, Chun Lian Song
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	angelica radix isatidis polysaccharides immune function rats Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/0928dbb656a04e7b979f21be66479025
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spelling	oai:doaj.org-article:0928dbb656a04e7b979f21be664790252021-11-12T05:25:37ZEffects of Two Polysaccharides From Traditional Chinese Medicines on Rat Immune Function2297-176910.3389/fvets.2021.703956https://doaj.org/article/0928dbb656a04e7b979f21be664790252021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.703956/fullhttps://doaj.org/toc/2297-1769Abstract: The aim is to study the immune function effect of two polysaccharides extracted from traditional Chinese herbs on rats. Ultrasonic-assisted extraction was used to extract the polysaccharide from traditional Chinese medicines. MTT assay was used to determine the effects of two polysaccharides on the conversion of pig peripheral T lymphocytes. For this, 24 Sprague–Dawley rats were selected for the clinical trial and divided into groups B (blank), CK (cyclophosphamide inhibitory control), AP (angelica polysaccharide), and RIP (radix isatidis polysaccharide). Except for group B, other groups can induce the immunodeficiency by using cyclophosphamide. Rats of the AP and RIP groups were given gavage of 1 mL of AP and RIP. The blood was sampled from the eyeball on days 0, 7, 14, 21, 28, and 35, respectively, to determine immune cells, IgG and IgM of immunoglobulin, body weight, and spleen index.Results: The average content of AP and RIP was 51.27 and 14.8%, and the extraction rate was 75.23 and 60.94%. The maximum stimulation index was 1.407 when the concentration of AP was 8,000 μg mL−1 and 1.5 when the concentration of RIP was 125 μg mL−1. Both kinds of polysaccharides can alleviate the decline of white blood cells, lymphocytes, monocytes, neutrophils, and serum IgG and IgM caused by cyclophosphamide. The two polysaccharides can regulate the rapid recovery of weight in immunosuppressed rats and increase the spleen index of immunosuppressed SD rats. The polysaccharides from the two traditional Chinese medicines can alleviate the immunosuppression caused by cyclophosphamide and promote the immune function of the body, which can be used as raw material resources of new veterinary medicine.Yu Lei WangXiang Hua ShuXue ZhangYong Bo LiuYa Jing ZhangTao LvXin HuangChun Lian SongFrontiers Media S.A.articleangelicaradix isatidispolysaccharidesimmune functionratsVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	angelica radix isatidis polysaccharides immune function rats Veterinary medicine SF600-1100
spellingShingle	angelica radix isatidis polysaccharides immune function rats Veterinary medicine SF600-1100 Yu Lei Wang Xiang Hua Shu Xue Zhang Yong Bo Liu Ya Jing Zhang Tao Lv Xin Huang Chun Lian Song Effects of Two Polysaccharides From Traditional Chinese Medicines on Rat Immune Function
description	Abstract: The aim is to study the immune function effect of two polysaccharides extracted from traditional Chinese herbs on rats. Ultrasonic-assisted extraction was used to extract the polysaccharide from traditional Chinese medicines. MTT assay was used to determine the effects of two polysaccharides on the conversion of pig peripheral T lymphocytes. For this, 24 Sprague–Dawley rats were selected for the clinical trial and divided into groups B (blank), CK (cyclophosphamide inhibitory control), AP (angelica polysaccharide), and RIP (radix isatidis polysaccharide). Except for group B, other groups can induce the immunodeficiency by using cyclophosphamide. Rats of the AP and RIP groups were given gavage of 1 mL of AP and RIP. The blood was sampled from the eyeball on days 0, 7, 14, 21, 28, and 35, respectively, to determine immune cells, IgG and IgM of immunoglobulin, body weight, and spleen index.Results: The average content of AP and RIP was 51.27 and 14.8%, and the extraction rate was 75.23 and 60.94%. The maximum stimulation index was 1.407 when the concentration of AP was 8,000 μg mL−1 and 1.5 when the concentration of RIP was 125 μg mL−1. Both kinds of polysaccharides can alleviate the decline of white blood cells, lymphocytes, monocytes, neutrophils, and serum IgG and IgM caused by cyclophosphamide. The two polysaccharides can regulate the rapid recovery of weight in immunosuppressed rats and increase the spleen index of immunosuppressed SD rats. The polysaccharides from the two traditional Chinese medicines can alleviate the immunosuppression caused by cyclophosphamide and promote the immune function of the body, which can be used as raw material resources of new veterinary medicine.
format	article
author	Yu Lei Wang Xiang Hua Shu Xue Zhang Yong Bo Liu Ya Jing Zhang Tao Lv Xin Huang Chun Lian Song
author_facet	Yu Lei Wang Xiang Hua Shu Xue Zhang Yong Bo Liu Ya Jing Zhang Tao Lv Xin Huang Chun Lian Song
author_sort	Yu Lei Wang
title	Effects of Two Polysaccharides From Traditional Chinese Medicines on Rat Immune Function
title_short	Effects of Two Polysaccharides From Traditional Chinese Medicines on Rat Immune Function
title_full	Effects of Two Polysaccharides From Traditional Chinese Medicines on Rat Immune Function
title_fullStr	Effects of Two Polysaccharides From Traditional Chinese Medicines on Rat Immune Function
title_full_unstemmed	Effects of Two Polysaccharides From Traditional Chinese Medicines on Rat Immune Function
title_sort	effects of two polysaccharides from traditional chinese medicines on rat immune function
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0928dbb656a04e7b979f21be66479025
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Effects of Two Polysaccharides From Traditional Chinese Medicines on Rat Immune Function

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