DYNAMICS OF HEAT SHOCK PROTEIN-70 SYNTHESIS IN LUNGS DEPENDS ON THE STAGE OF EXPERIMENTAL RESPIRATORY DISTRESS SYNDROME

Abstract. Acute respiratory distress syndrome (ARDS) was reproduced in a rat model, by means of intratracheal instillation of granulocyte lysates (a method protected by Russian patent). Expression of HSP-70 in lung cells was determined by immunohistochemical technique at each ARDS stage. A significa...

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Autores principales:	E. V. Prutkina, A. V. Sepp, N. N. Tsybikov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	respiratory distress syndrome experimental lung cells hsp-70 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/093cf5acfd7442fca5e10f5b9704368c
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spelling	oai:doaj.org-article:093cf5acfd7442fca5e10f5b9704368c2021-11-18T08:03:43ZDYNAMICS OF HEAT SHOCK PROTEIN-70 SYNTHESIS IN LUNGS DEPENDS ON THE STAGE OF EXPERIMENTAL RESPIRATORY DISTRESS SYNDROME1563-06252313-741X10.15789/1563-0625-2013-4-335-342https://doaj.org/article/093cf5acfd7442fca5e10f5b9704368c2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/644https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Acute respiratory distress syndrome (ARDS) was reproduced in a rat model, by means of intratracheal instillation of granulocyte lysates (a method protected by Russian patent). Expression of HSP-70 in lung cells was determined by immunohistochemical technique at each ARDS stage. A significant increase of HSP-70 expression by all cell types was revealed during exudative stage, being more intensive in alveolocytes type 1, and less expressed in endothelium. During proliferative stage of the disorder, a decreased HSP-70 expression was noted in all cell populations. At these terms, it proved to be high in neutrophils and alveveolocytes type 1, whereas lower expression was registered in endothelium. At fibrotic stage, HSP-70 synthesis remained at high levels in neutrophils, macrophages, fibroblasts and alveolocytes type 1. Endothelium and alveolocytes type 2 exhibited a recurrent increase at fibrotic stage of ARDS, however it did not reach the values typical to the initial stage of the syndrome.E. V. PrutkinaA. V. SeppN. N. TsybikovSPb RAACIarticlerespiratory distress syndromeexperimentallung cellshsp-70Immunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 15, Iss 4, Pp 335-342 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	respiratory distress syndrome experimental lung cells hsp-70 Immunologic diseases. Allergy RC581-607
spellingShingle	respiratory distress syndrome experimental lung cells hsp-70 Immunologic diseases. Allergy RC581-607 E. V. Prutkina A. V. Sepp N. N. Tsybikov DYNAMICS OF HEAT SHOCK PROTEIN-70 SYNTHESIS IN LUNGS DEPENDS ON THE STAGE OF EXPERIMENTAL RESPIRATORY DISTRESS SYNDROME
description	Abstract. Acute respiratory distress syndrome (ARDS) was reproduced in a rat model, by means of intratracheal instillation of granulocyte lysates (a method protected by Russian patent). Expression of HSP-70 in lung cells was determined by immunohistochemical technique at each ARDS stage. A significant increase of HSP-70 expression by all cell types was revealed during exudative stage, being more intensive in alveolocytes type 1, and less expressed in endothelium. During proliferative stage of the disorder, a decreased HSP-70 expression was noted in all cell populations. At these terms, it proved to be high in neutrophils and alveveolocytes type 1, whereas lower expression was registered in endothelium. At fibrotic stage, HSP-70 synthesis remained at high levels in neutrophils, macrophages, fibroblasts and alveolocytes type 1. Endothelium and alveolocytes type 2 exhibited a recurrent increase at fibrotic stage of ARDS, however it did not reach the values typical to the initial stage of the syndrome.
format	article
author	E. V. Prutkina A. V. Sepp N. N. Tsybikov
author_facet	E. V. Prutkina A. V. Sepp N. N. Tsybikov
author_sort	E. V. Prutkina
title	DYNAMICS OF HEAT SHOCK PROTEIN-70 SYNTHESIS IN LUNGS DEPENDS ON THE STAGE OF EXPERIMENTAL RESPIRATORY DISTRESS SYNDROME
title_short	DYNAMICS OF HEAT SHOCK PROTEIN-70 SYNTHESIS IN LUNGS DEPENDS ON THE STAGE OF EXPERIMENTAL RESPIRATORY DISTRESS SYNDROME
title_full	DYNAMICS OF HEAT SHOCK PROTEIN-70 SYNTHESIS IN LUNGS DEPENDS ON THE STAGE OF EXPERIMENTAL RESPIRATORY DISTRESS SYNDROME
title_fullStr	DYNAMICS OF HEAT SHOCK PROTEIN-70 SYNTHESIS IN LUNGS DEPENDS ON THE STAGE OF EXPERIMENTAL RESPIRATORY DISTRESS SYNDROME
title_full_unstemmed	DYNAMICS OF HEAT SHOCK PROTEIN-70 SYNTHESIS IN LUNGS DEPENDS ON THE STAGE OF EXPERIMENTAL RESPIRATORY DISTRESS SYNDROME
title_sort	dynamics of heat shock protein-70 synthesis in lungs depends on the stage of experimental respiratory distress syndrome
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/093cf5acfd7442fca5e10f5b9704368c
work_keys_str_mv	AT evprutkina dynamicsofheatshockprotein70synthesisinlungsdependsonthestageofexperimentalrespiratorydistresssyndrome AT avsepp dynamicsofheatshockprotein70synthesisinlungsdependsonthestageofexperimentalrespiratorydistresssyndrome AT nntsybikov dynamicsofheatshockprotein70synthesisinlungsdependsonthestageofexperimentalrespiratorydistresssyndrome
_version_	1718422461992665088

DYNAMICS OF HEAT SHOCK PROTEIN-70 SYNTHESIS IN LUNGS DEPENDS ON THE STAGE OF EXPERIMENTAL RESPIRATORY DISTRESS SYNDROME

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