The Complete Genome Sequence of <named-content content-type="genus-species">Clostridium aceticum</named-content>: a Missing Link between Rnf- and Cytochrome-Containing Autotrophic Acetogens

ABSTRACT Clostridium aceticum was the first isolated autotrophic acetogen, converting CO2 plus H2 or syngas to acetate. Its genome has now been completely sequenced and consists of a 4.2-Mbp chromosome and a small circular plasmid of 5.7 kbp. Sequence analysis revealed major differences from other a...

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Autores principales:	Anja Poehlein, Martin Cebulla, Marcus M. Ilg, Frank R. Bengelsdorf, Bettina Schiel-Bengelsdorf, Gregg Whited, Jan R. Andreesen, Gerhard Gottschalk, Rolf Daniel, Peter Dürre
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2015
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/094d27ca73ff4eb9bdc6fee72b345a2f
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spelling	oai:doaj.org-article:094d27ca73ff4eb9bdc6fee72b345a2f2021-11-15T15:41:30ZThe Complete Genome Sequence of <named-content content-type="genus-species">Clostridium aceticum</named-content>: a Missing Link between Rnf- and Cytochrome-Containing Autotrophic Acetogens10.1128/mBio.01168-152150-7511https://doaj.org/article/094d27ca73ff4eb9bdc6fee72b345a2f2015-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01168-15https://doaj.org/toc/2150-7511ABSTRACT Clostridium aceticum was the first isolated autotrophic acetogen, converting CO2 plus H2 or syngas to acetate. Its genome has now been completely sequenced and consists of a 4.2-Mbp chromosome and a small circular plasmid of 5.7 kbp. Sequence analysis revealed major differences from other autotrophic acetogens. C. aceticum contains an Rnf complex for energy conservation (via pumping protons or sodium ions). Such systems have also been found in C. ljungdahlii and Acetobacterium woodii. However, C. aceticum also contains a cytochrome, as does Moorella thermoacetica, which has been proposed to be involved in the generation of a proton gradient. Thus, C. aceticum seems to represent a link between Rnf- and cytochrome-containing autotrophic acetogens. In C. aceticum, however, the cytochrome is probably not involved in an electron transport chain that leads to proton translocation, as no genes for quinone biosynthesis are present in the genome. IMPORTANCE Autotrophic acetogenic bacteria are receiving more and more industrial focus, as CO2 plus H2 as well as syngas are interesting new substrates for biotechnological processes. They are both cheap and abundant, and their use, if it results in sustainable products, also leads to reduction of greenhouse gases. Clostridium aceticum can use both gas mixtures, is phylogenetically not closely related to the commonly used species, and may thus become an even more attractive workhorse. In addition, its energy metabolism, which is characterized here, and the ability to synthesize cytochromes might offer new targets for improving the ATP yield by metabolic engineering and thus allow use of C. aceticum for production of compounds by pathways that currently present challenges for energy-limited acetogens.Anja PoehleinMartin CebullaMarcus M. IlgFrank R. BengelsdorfBettina Schiel-BengelsdorfGregg WhitedJan R. AndreesenGerhard GottschalkRolf DanielPeter DürreAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 6, Iss 5 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Anja Poehlein Martin Cebulla Marcus M. Ilg Frank R. Bengelsdorf Bettina Schiel-Bengelsdorf Gregg Whited Jan R. Andreesen Gerhard Gottschalk Rolf Daniel Peter Dürre The Complete Genome Sequence of <named-content content-type="genus-species">Clostridium aceticum</named-content>: a Missing Link between Rnf- and Cytochrome-Containing Autotrophic Acetogens
description	ABSTRACT Clostridium aceticum was the first isolated autotrophic acetogen, converting CO2 plus H2 or syngas to acetate. Its genome has now been completely sequenced and consists of a 4.2-Mbp chromosome and a small circular plasmid of 5.7 kbp. Sequence analysis revealed major differences from other autotrophic acetogens. C. aceticum contains an Rnf complex for energy conservation (via pumping protons or sodium ions). Such systems have also been found in C. ljungdahlii and Acetobacterium woodii. However, C. aceticum also contains a cytochrome, as does Moorella thermoacetica, which has been proposed to be involved in the generation of a proton gradient. Thus, C. aceticum seems to represent a link between Rnf- and cytochrome-containing autotrophic acetogens. In C. aceticum, however, the cytochrome is probably not involved in an electron transport chain that leads to proton translocation, as no genes for quinone biosynthesis are present in the genome. IMPORTANCE Autotrophic acetogenic bacteria are receiving more and more industrial focus, as CO2 plus H2 as well as syngas are interesting new substrates for biotechnological processes. They are both cheap and abundant, and their use, if it results in sustainable products, also leads to reduction of greenhouse gases. Clostridium aceticum can use both gas mixtures, is phylogenetically not closely related to the commonly used species, and may thus become an even more attractive workhorse. In addition, its energy metabolism, which is characterized here, and the ability to synthesize cytochromes might offer new targets for improving the ATP yield by metabolic engineering and thus allow use of C. aceticum for production of compounds by pathways that currently present challenges for energy-limited acetogens.
format	article
author	Anja Poehlein Martin Cebulla Marcus M. Ilg Frank R. Bengelsdorf Bettina Schiel-Bengelsdorf Gregg Whited Jan R. Andreesen Gerhard Gottschalk Rolf Daniel Peter Dürre
author_facet	Anja Poehlein Martin Cebulla Marcus M. Ilg Frank R. Bengelsdorf Bettina Schiel-Bengelsdorf Gregg Whited Jan R. Andreesen Gerhard Gottschalk Rolf Daniel Peter Dürre
author_sort	Anja Poehlein
title	The Complete Genome Sequence of <named-content content-type="genus-species">Clostridium aceticum</named-content>: a Missing Link between Rnf- and Cytochrome-Containing Autotrophic Acetogens
title_short	The Complete Genome Sequence of <named-content content-type="genus-species">Clostridium aceticum</named-content>: a Missing Link between Rnf- and Cytochrome-Containing Autotrophic Acetogens
title_full	The Complete Genome Sequence of <named-content content-type="genus-species">Clostridium aceticum</named-content>: a Missing Link between Rnf- and Cytochrome-Containing Autotrophic Acetogens
title_fullStr	The Complete Genome Sequence of <named-content content-type="genus-species">Clostridium aceticum</named-content>: a Missing Link between Rnf- and Cytochrome-Containing Autotrophic Acetogens
title_full_unstemmed	The Complete Genome Sequence of <named-content content-type="genus-species">Clostridium aceticum</named-content>: a Missing Link between Rnf- and Cytochrome-Containing Autotrophic Acetogens
title_sort	complete genome sequence of <named-content content-type="genus-species">clostridium aceticum</named-content>: a missing link between rnf- and cytochrome-containing autotrophic acetogens
publisher	American Society for Microbiology
publishDate	2015
url	https://doaj.org/article/094d27ca73ff4eb9bdc6fee72b345a2f
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The Complete Genome Sequence of <named-content content-type="genus-species">Clostridium aceticum</named-content>: a Missing Link between Rnf- and Cytochrome-Containing Autotrophic Acetogens

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