Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations

Hypertrophic cardiomyopathy (HCM) is a common inherited heart disease with a prevalence of about 0.2%. HCM is typically caused by mutations in genes encoding sarcomere or sarcomere-associated proteins. Here, we characterized induced pluripotent stem cell (iPSC) lines generated from the peripheral bl...

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Main Authors:	Shane Rui Zhao, Mengcheng Shen, Chelsea Lee, Yanjun Zha, Julio V. Guevara, Matthew T. Wheeler, Joseph C. Wu
Format:	article
Language:	EN
Published:	Elsevier 2021
Subjects:	Biology (General) QH301-705.5
Online Access:	https://doaj.org/article/0950bb3b3c6e40cfbdf78fc8d624010c
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spelling	oai:doaj.org-article:0950bb3b3c6e40cfbdf78fc8d624010c2021-11-18T04:46:23ZGeneration of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations1873-506110.1016/j.scr.2021.102597https://doaj.org/article/0950bb3b3c6e40cfbdf78fc8d624010c2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S187350612100444Xhttps://doaj.org/toc/1873-5061Hypertrophic cardiomyopathy (HCM) is a common inherited heart disease with a prevalence of about 0.2%. HCM is typically caused by mutations in genes encoding sarcomere or sarcomere-associated proteins. Here, we characterized induced pluripotent stem cell (iPSC) lines generated from the peripheral blood mononuclear cells of three HCM patients each carrying c.433C > T, c.610C > T, or c.235C > T mutation in the TNNI3 gene by non-integrated Sendai virus. All of the three lines exhibited normal morphology, expression of pluripotent markers, stable karyotype, and the potential of trilineage differentiation. The cardiomyocytes differentiated from these iPSC lines can serve as useful tools to model HCM in vitro.Shane Rui ZhaoMengcheng ShenChelsea LeeYanjun ZhaJulio V. GuevaraMatthew T. WheelerJoseph C. WuElsevierarticleBiology (General)QH301-705.5ENStem Cell Research, Vol 57, Iss , Pp 102597- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Shane Rui Zhao Mengcheng Shen Chelsea Lee Yanjun Zha Julio V. Guevara Matthew T. Wheeler Joseph C. Wu Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations
description	Hypertrophic cardiomyopathy (HCM) is a common inherited heart disease with a prevalence of about 0.2%. HCM is typically caused by mutations in genes encoding sarcomere or sarcomere-associated proteins. Here, we characterized induced pluripotent stem cell (iPSC) lines generated from the peripheral blood mononuclear cells of three HCM patients each carrying c.433C > T, c.610C > T, or c.235C > T mutation in the TNNI3 gene by non-integrated Sendai virus. All of the three lines exhibited normal morphology, expression of pluripotent markers, stable karyotype, and the potential of trilineage differentiation. The cardiomyocytes differentiated from these iPSC lines can serve as useful tools to model HCM in vitro.
format	article
author	Shane Rui Zhao Mengcheng Shen Chelsea Lee Yanjun Zha Julio V. Guevara Matthew T. Wheeler Joseph C. Wu
author_facet	Shane Rui Zhao Mengcheng Shen Chelsea Lee Yanjun Zha Julio V. Guevara Matthew T. Wheeler Joseph C. Wu
author_sort	Shane Rui Zhao
title	Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations
title_short	Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations
title_full	Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations
title_fullStr	Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations
title_full_unstemmed	Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations
title_sort	generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying tnni3 mutations
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/0950bb3b3c6e40cfbdf78fc8d624010c
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_version_	1718425018658979840

Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations

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