Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells.

Lipid droplets (LDs) are spherical accumulations of apolar lipids and other hydrophobic substances and are generally surrounded by a thin cortical layer of specific amphiphilic proteins (APs). These APs segregate the LDs from the mostly polar components of the cytoplasm. We have studied LDs in epith...

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Autores principales:	Hans Heid, Steffen Rickelt, Ralf Zimbelmann, Stefanie Winter, Heiderose Schumacher, Yvette Dörflinger
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0950c1e9dc7543f0b888dcce8c394e99
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spelling	oai:doaj.org-article:0950c1e9dc7543f0b888dcce8c394e992021-11-18T07:44:48ZLipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells.1932-620310.1371/journal.pone.0063061https://doaj.org/article/0950c1e9dc7543f0b888dcce8c394e992013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23704888/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Lipid droplets (LDs) are spherical accumulations of apolar lipids and other hydrophobic substances and are generally surrounded by a thin cortical layer of specific amphiphilic proteins (APs). These APs segregate the LDs from the mostly polar components of the cytoplasm. We have studied LDs in epithelium-derived cell cultures and in particular characterized proteins from the perilipin (PLIN) gene family - in mammals consisting of the proteins Perilipin, Adipophilin, TIP47, S3-12 and MLDP/OXPAT (PLIN 1-5). Using a large number of newly generated and highly specific mono- and polyclonal antibodies specific for individual APs, and using improved LD isolation methods, we have enriched and characterized APs in greater detail and purity. The majority of lipid-AP complexes could be obtained in the top layer fractions of density gradient centrifugation separations of cultured cells, but APs could also be detected in other fractions within such separations. The differently sized LD complexes were analyzed using various biochemical methods and mass spectrometry as well as immunofluorescence and electron- in particular immunoelectron-microscopy. Moreover, by immunoprecipitation, protein-protein binding assays and by immunoelectron microscopy we identified a direct linkage between LD-binding proteins and the intermediate-sized filaments (IF) cytokeratins 8 and 18 (also designated as keratins K8 and K18). Specifically, in gradient fractions of higher density supposedly containing small LDs, we received as co-precipitations cytidylyl-, palmitoyl- and cholesterol transferases and other specific enzymes involved in lipid metabolism. So far, common proteomic studies have used LDs from top layer fractions only and did not report on these transferases and other enzymes. In addition to findings of short alternating hydrophobic/hydrophilic segments within the PLIN protein family, we propose and discuss a model for the interaction of LD-coating APs with IF proteins.Hans HeidSteffen RickeltRalf ZimbelmannStefanie WinterHeiderose SchumacherYvette DörflingerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63061 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hans Heid Steffen Rickelt Ralf Zimbelmann Stefanie Winter Heiderose Schumacher Yvette Dörflinger Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells.
description	Lipid droplets (LDs) are spherical accumulations of apolar lipids and other hydrophobic substances and are generally surrounded by a thin cortical layer of specific amphiphilic proteins (APs). These APs segregate the LDs from the mostly polar components of the cytoplasm. We have studied LDs in epithelium-derived cell cultures and in particular characterized proteins from the perilipin (PLIN) gene family - in mammals consisting of the proteins Perilipin, Adipophilin, TIP47, S3-12 and MLDP/OXPAT (PLIN 1-5). Using a large number of newly generated and highly specific mono- and polyclonal antibodies specific for individual APs, and using improved LD isolation methods, we have enriched and characterized APs in greater detail and purity. The majority of lipid-AP complexes could be obtained in the top layer fractions of density gradient centrifugation separations of cultured cells, but APs could also be detected in other fractions within such separations. The differently sized LD complexes were analyzed using various biochemical methods and mass spectrometry as well as immunofluorescence and electron- in particular immunoelectron-microscopy. Moreover, by immunoprecipitation, protein-protein binding assays and by immunoelectron microscopy we identified a direct linkage between LD-binding proteins and the intermediate-sized filaments (IF) cytokeratins 8 and 18 (also designated as keratins K8 and K18). Specifically, in gradient fractions of higher density supposedly containing small LDs, we received as co-precipitations cytidylyl-, palmitoyl- and cholesterol transferases and other specific enzymes involved in lipid metabolism. So far, common proteomic studies have used LDs from top layer fractions only and did not report on these transferases and other enzymes. In addition to findings of short alternating hydrophobic/hydrophilic segments within the PLIN protein family, we propose and discuss a model for the interaction of LD-coating APs with IF proteins.
format	article
author	Hans Heid Steffen Rickelt Ralf Zimbelmann Stefanie Winter Heiderose Schumacher Yvette Dörflinger
author_facet	Hans Heid Steffen Rickelt Ralf Zimbelmann Stefanie Winter Heiderose Schumacher Yvette Dörflinger
author_sort	Hans Heid
title	Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells.
title_short	Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells.
title_full	Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells.
title_fullStr	Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells.
title_full_unstemmed	Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells.
title_sort	lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/0950c1e9dc7543f0b888dcce8c394e99
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Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells.

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