Alterations of thyroid microbiota across different thyroid microhabitats in patients with thyroid carcinoma

Abstract Background In recent years, the incidence rate of Thyroid carcinoma (TC) has been increasing worldwide. Thus, research on factors of TC carcinogenesis may promote TC prevention and decrease the incidence rate. There are several studies targeting the correlation between gut microbiota and th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Daofeng Dai, Yan Yang, Yong Yang, Tianfeng Dang, Jiansheng Xiao, Weibin Wang, Lisong Teng, Juan Xu, Jing Ye, Hongqun Jiang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Thyroid cancer Microbiome Lymph node metastasis Biomarker Sphingomonas Medicine R
Acceso en línea:	https://doaj.org/article/095b9e0c0aa6402ca7c1c785e1930dc3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:095b9e0c0aa6402ca7c1c785e1930dc3
record_format	dspace
spelling	oai:doaj.org-article:095b9e0c0aa6402ca7c1c785e1930dc32021-12-05T12:05:59ZAlterations of thyroid microbiota across different thyroid microhabitats in patients with thyroid carcinoma10.1186/s12967-021-03167-91479-5876https://doaj.org/article/095b9e0c0aa6402ca7c1c785e1930dc32021-11-01T00:00:00Zhttps://doi.org/10.1186/s12967-021-03167-9https://doaj.org/toc/1479-5876Abstract Background In recent years, the incidence rate of Thyroid carcinoma (TC) has been increasing worldwide. Thus, research on factors of TC carcinogenesis may promote TC prevention and decrease the incidence rate. There are several studies targeting the correlation between gut microbiota and thyroid disease. Carcinogenesis of several malignancies is influenced by microbiota. However, thyroid microbiome of TC has not been revealed. This study investigated thyroid microbiota in different TC microhabitats. Methods We performed 16s rRNA gene sequencing using tumor tissues and matched peritumor tissues from 30 patients with TC to characterize thyroid microbiota. Results The richness and diversity of thyroid microbiota were lower in TC tumor samples than in matched peritumor tissues. At the genus level, the core microbiota of thyroid included Sphingomonas, Comamonas, Acinetobacter, Pseudomonas, Microvirgula, and Soonwooa. The abundance of Sphingomonas and Aeromonas was significantly increased in tumor tissues, while the abundance of Comamonas, Acinetobacter, and Peptostreptococcus was significantly enhanced in peritumor tissues. The combination of Comamonas and Sphingomonas could discriminate tumor samples from peritumor samples with an area under the curve (AUC) of 0.981 (95% confidence interval [CI] 0.949–1.000). The abundance of Sphingomonas was significantly higher in N1 stage than in N0 stage. Sphingomonas could distinguish between N0 and N1 stage with an AUC of 0.964 (95% CI 0.907–1.000). Conclusions The microbial diversity and composition were significantly different between peritumor and tumor microhabitats from patients with TC, which may eventually affect TC carcinogenesis and progression. The combination of Comamonas and Sphingomonas could serve as a powerful biomarker for discrimination between tumor and peritumor tissues. Furthermore, the higher abundance of Sphingomonas was correlated with lymph node metastasis, indicating that the abundance of Sphingomonas may indicate a poor prognosis for TC patients, and Sphingomonas may play a role in promoting TC progression.Daofeng DaiYan YangYong YangTianfeng DangJiansheng XiaoWeibin WangLisong TengJuan XuJing YeHongqun JiangBMCarticleThyroid cancerMicrobiomeLymph node metastasisBiomarkerSphingomonasMedicineRENJournal of Translational Medicine, Vol 19, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Thyroid cancer Microbiome Lymph node metastasis Biomarker Sphingomonas Medicine R
spellingShingle	Thyroid cancer Microbiome Lymph node metastasis Biomarker Sphingomonas Medicine R Daofeng Dai Yan Yang Yong Yang Tianfeng Dang Jiansheng Xiao Weibin Wang Lisong Teng Juan Xu Jing Ye Hongqun Jiang Alterations of thyroid microbiota across different thyroid microhabitats in patients with thyroid carcinoma
description	Abstract Background In recent years, the incidence rate of Thyroid carcinoma (TC) has been increasing worldwide. Thus, research on factors of TC carcinogenesis may promote TC prevention and decrease the incidence rate. There are several studies targeting the correlation between gut microbiota and thyroid disease. Carcinogenesis of several malignancies is influenced by microbiota. However, thyroid microbiome of TC has not been revealed. This study investigated thyroid microbiota in different TC microhabitats. Methods We performed 16s rRNA gene sequencing using tumor tissues and matched peritumor tissues from 30 patients with TC to characterize thyroid microbiota. Results The richness and diversity of thyroid microbiota were lower in TC tumor samples than in matched peritumor tissues. At the genus level, the core microbiota of thyroid included Sphingomonas, Comamonas, Acinetobacter, Pseudomonas, Microvirgula, and Soonwooa. The abundance of Sphingomonas and Aeromonas was significantly increased in tumor tissues, while the abundance of Comamonas, Acinetobacter, and Peptostreptococcus was significantly enhanced in peritumor tissues. The combination of Comamonas and Sphingomonas could discriminate tumor samples from peritumor samples with an area under the curve (AUC) of 0.981 (95% confidence interval [CI] 0.949–1.000). The abundance of Sphingomonas was significantly higher in N1 stage than in N0 stage. Sphingomonas could distinguish between N0 and N1 stage with an AUC of 0.964 (95% CI 0.907–1.000). Conclusions The microbial diversity and composition were significantly different between peritumor and tumor microhabitats from patients with TC, which may eventually affect TC carcinogenesis and progression. The combination of Comamonas and Sphingomonas could serve as a powerful biomarker for discrimination between tumor and peritumor tissues. Furthermore, the higher abundance of Sphingomonas was correlated with lymph node metastasis, indicating that the abundance of Sphingomonas may indicate a poor prognosis for TC patients, and Sphingomonas may play a role in promoting TC progression.
format	article
author	Daofeng Dai Yan Yang Yong Yang Tianfeng Dang Jiansheng Xiao Weibin Wang Lisong Teng Juan Xu Jing Ye Hongqun Jiang
author_facet	Daofeng Dai Yan Yang Yong Yang Tianfeng Dang Jiansheng Xiao Weibin Wang Lisong Teng Juan Xu Jing Ye Hongqun Jiang
author_sort	Daofeng Dai
title	Alterations of thyroid microbiota across different thyroid microhabitats in patients with thyroid carcinoma
title_short	Alterations of thyroid microbiota across different thyroid microhabitats in patients with thyroid carcinoma
title_full	Alterations of thyroid microbiota across different thyroid microhabitats in patients with thyroid carcinoma
title_fullStr	Alterations of thyroid microbiota across different thyroid microhabitats in patients with thyroid carcinoma
title_full_unstemmed	Alterations of thyroid microbiota across different thyroid microhabitats in patients with thyroid carcinoma
title_sort	alterations of thyroid microbiota across different thyroid microhabitats in patients with thyroid carcinoma
publisher	BMC
publishDate	2021
url	https://doaj.org/article/095b9e0c0aa6402ca7c1c785e1930dc3
work_keys_str_mv	AT daofengdai alterationsofthyroidmicrobiotaacrossdifferentthyroidmicrohabitatsinpatientswiththyroidcarcinoma AT yanyang alterationsofthyroidmicrobiotaacrossdifferentthyroidmicrohabitatsinpatientswiththyroidcarcinoma AT yongyang alterationsofthyroidmicrobiotaacrossdifferentthyroidmicrohabitatsinpatientswiththyroidcarcinoma AT tianfengdang alterationsofthyroidmicrobiotaacrossdifferentthyroidmicrohabitatsinpatientswiththyroidcarcinoma AT jianshengxiao alterationsofthyroidmicrobiotaacrossdifferentthyroidmicrohabitatsinpatientswiththyroidcarcinoma AT weibinwang alterationsofthyroidmicrobiotaacrossdifferentthyroidmicrohabitatsinpatientswiththyroidcarcinoma AT lisongteng alterationsofthyroidmicrobiotaacrossdifferentthyroidmicrohabitatsinpatientswiththyroidcarcinoma AT juanxu alterationsofthyroidmicrobiotaacrossdifferentthyroidmicrohabitatsinpatientswiththyroidcarcinoma AT jingye alterationsofthyroidmicrobiotaacrossdifferentthyroidmicrohabitatsinpatientswiththyroidcarcinoma AT hongqunjiang alterationsofthyroidmicrobiotaacrossdifferentthyroidmicrohabitatsinpatientswiththyroidcarcinoma
_version_	1718372236220432384

Alterations of thyroid microbiota across different thyroid microhabitats in patients with thyroid carcinoma

Ejemplares similares