Visualizing Nudivirus Assembly and Egress

ABSTRACT Enveloped viruses hijack cellular membranes in order to provide the necessary material for virion assembly. In particular, viruses that replicate and assemble inside the nucleus have developed special approaches to modify the nuclear landscape for their advantage. We used electron microscop...

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Autores principales:	Sailakshmi Velamoor, Allan Mitchell, Bruno M. Humbel, WonMo Kim, Charlotte Pushparajan, Gabriel Visnovsky, Laura N. Burga, Mihnea Bostina
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	electron microscopy enveloped viruses nudiviruses viral assembly Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/095d8e76b1d748f289f1c836a2259964
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spelling	oai:doaj.org-article:095d8e76b1d748f289f1c836a22599642021-11-15T15:56:43ZVisualizing Nudivirus Assembly and Egress10.1128/mBio.01333-202150-7511https://doaj.org/article/095d8e76b1d748f289f1c836a22599642020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01333-20https://doaj.org/toc/2150-7511ABSTRACT Enveloped viruses hijack cellular membranes in order to provide the necessary material for virion assembly. In particular, viruses that replicate and assemble inside the nucleus have developed special approaches to modify the nuclear landscape for their advantage. We used electron microscopy to investigate cellular changes occurring during nudivirus infection and we characterized a unique mechanism for assembly, packaging, and transport of new virions across the nuclear membrane and through the cytoplasm. Our three-dimensional reconstructions describe the complex remodeling of the nuclear membrane necessary to release vesicle-associated viruses into the cytoplasm. This is the first report of nuclear morphological reconfigurations that occur during nudiviral infection. IMPORTANCE The dynamics of nuclear envelope has a critical role in multiple cellular processes. However, little is known regarding the structural changes occurring inside the nucleus or at the inner and outer nuclear membranes. For viruses assembling inside the nucleus, remodeling of the intranuclear membrane plays an important role in the process of virion assembly. Here, we monitored the changes associated with viral infection in the case of nudiviruses. Our data revealed dramatic membrane remodeling inside the nuclear compartment during infection with Oryctes rhinoceros nudivirus, an important biocontrol agent against coconut rhinoceros beetle, a devastating pest for coconut and oil palm trees. Based on these findings, we propose a model for nudivirus assembly in which nuclear packaging occurs in fully enveloped virions.Sailakshmi VelamoorAllan MitchellBruno M. HumbelWonMo KimCharlotte PushparajanGabriel VisnovskyLaura N. BurgaMihnea BostinaAmerican Society for Microbiologyarticleelectron microscopyenveloped virusesnudivirusesviral assemblyMicrobiologyQR1-502ENmBio, Vol 11, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	electron microscopy enveloped viruses nudiviruses viral assembly Microbiology QR1-502
spellingShingle	electron microscopy enveloped viruses nudiviruses viral assembly Microbiology QR1-502 Sailakshmi Velamoor Allan Mitchell Bruno M. Humbel WonMo Kim Charlotte Pushparajan Gabriel Visnovsky Laura N. Burga Mihnea Bostina Visualizing Nudivirus Assembly and Egress
description	ABSTRACT Enveloped viruses hijack cellular membranes in order to provide the necessary material for virion assembly. In particular, viruses that replicate and assemble inside the nucleus have developed special approaches to modify the nuclear landscape for their advantage. We used electron microscopy to investigate cellular changes occurring during nudivirus infection and we characterized a unique mechanism for assembly, packaging, and transport of new virions across the nuclear membrane and through the cytoplasm. Our three-dimensional reconstructions describe the complex remodeling of the nuclear membrane necessary to release vesicle-associated viruses into the cytoplasm. This is the first report of nuclear morphological reconfigurations that occur during nudiviral infection. IMPORTANCE The dynamics of nuclear envelope has a critical role in multiple cellular processes. However, little is known regarding the structural changes occurring inside the nucleus or at the inner and outer nuclear membranes. For viruses assembling inside the nucleus, remodeling of the intranuclear membrane plays an important role in the process of virion assembly. Here, we monitored the changes associated with viral infection in the case of nudiviruses. Our data revealed dramatic membrane remodeling inside the nuclear compartment during infection with Oryctes rhinoceros nudivirus, an important biocontrol agent against coconut rhinoceros beetle, a devastating pest for coconut and oil palm trees. Based on these findings, we propose a model for nudivirus assembly in which nuclear packaging occurs in fully enveloped virions.
format	article
author	Sailakshmi Velamoor Allan Mitchell Bruno M. Humbel WonMo Kim Charlotte Pushparajan Gabriel Visnovsky Laura N. Burga Mihnea Bostina
author_facet	Sailakshmi Velamoor Allan Mitchell Bruno M. Humbel WonMo Kim Charlotte Pushparajan Gabriel Visnovsky Laura N. Burga Mihnea Bostina
author_sort	Sailakshmi Velamoor
title	Visualizing Nudivirus Assembly and Egress
title_short	Visualizing Nudivirus Assembly and Egress
title_full	Visualizing Nudivirus Assembly and Egress
title_fullStr	Visualizing Nudivirus Assembly and Egress
title_full_unstemmed	Visualizing Nudivirus Assembly and Egress
title_sort	visualizing nudivirus assembly and egress
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/095d8e76b1d748f289f1c836a2259964
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Visualizing Nudivirus Assembly and Egress

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