Phenotypic plasticity and a new small molecule are involved in a fungal-bacterial interaction

Abstract Nitrogen-fixing bacteria have been extensively studied in the context of interactions with their host plants; however, little is known about the phenotypic plasticity of these microorganisms in nonmutualistic interactions with other eukaryotes. A dual-species coculture model was developed b...

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Autores principales:	Andrés Andrade-Domínguez, Abigail Trejo-Hernández, Carmen Vargas-Lagunas, Sergio Encarnación-Guevara
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/09613681a6ad4f08a53555218badea54
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spelling	oai:doaj.org-article:09613681a6ad4f08a53555218badea542021-12-02T17:17:40ZPhenotypic plasticity and a new small molecule are involved in a fungal-bacterial interaction10.1038/s41598-021-98474-y2045-2322https://doaj.org/article/09613681a6ad4f08a53555218badea542021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98474-yhttps://doaj.org/toc/2045-2322Abstract Nitrogen-fixing bacteria have been extensively studied in the context of interactions with their host plants; however, little is known about the phenotypic plasticity of these microorganisms in nonmutualistic interactions with other eukaryotes. A dual-species coculture model was developed by using the plant symbiotic bacterium Rhizobium etli and the well-studied eukaryote Saccharomyces cerevisiae as a tractable system to explore the molecular mechanisms used by R. etli in nonmutual interactions. Here, we show that the fungus promotes the growth of the bacterium and that together, these organisms form a mixed biofilm whose biomass is ~ 3 times greater and is more structured than that of either single-species biofilm. We found that these biofilm traits are dependent on a symbiotic plasmid encoding elements involved in the phenotypic plasticity of the bacterium, mitochondrial function and in the production of a yeast-secreted sophoroside. Interestingly, the promoters of 3 genes that are key in plant bacteria-interaction (nifH, fixA and nodA) were induced when R. etli coexists with yeast. These results show that investigating interactions between species that do not naturally coexist is a new approach to discover gene functions and specialized metabolites in model organisms.Andrés Andrade-DomínguezAbigail Trejo-HernándezCarmen Vargas-LagunasSergio Encarnación-GuevaraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrés Andrade-Domínguez Abigail Trejo-Hernández Carmen Vargas-Lagunas Sergio Encarnación-Guevara Phenotypic plasticity and a new small molecule are involved in a fungal-bacterial interaction
description	Abstract Nitrogen-fixing bacteria have been extensively studied in the context of interactions with their host plants; however, little is known about the phenotypic plasticity of these microorganisms in nonmutualistic interactions with other eukaryotes. A dual-species coculture model was developed by using the plant symbiotic bacterium Rhizobium etli and the well-studied eukaryote Saccharomyces cerevisiae as a tractable system to explore the molecular mechanisms used by R. etli in nonmutual interactions. Here, we show that the fungus promotes the growth of the bacterium and that together, these organisms form a mixed biofilm whose biomass is ~ 3 times greater and is more structured than that of either single-species biofilm. We found that these biofilm traits are dependent on a symbiotic plasmid encoding elements involved in the phenotypic plasticity of the bacterium, mitochondrial function and in the production of a yeast-secreted sophoroside. Interestingly, the promoters of 3 genes that are key in plant bacteria-interaction (nifH, fixA and nodA) were induced when R. etli coexists with yeast. These results show that investigating interactions between species that do not naturally coexist is a new approach to discover gene functions and specialized metabolites in model organisms.
format	article
author	Andrés Andrade-Domínguez Abigail Trejo-Hernández Carmen Vargas-Lagunas Sergio Encarnación-Guevara
author_facet	Andrés Andrade-Domínguez Abigail Trejo-Hernández Carmen Vargas-Lagunas Sergio Encarnación-Guevara
author_sort	Andrés Andrade-Domínguez
title	Phenotypic plasticity and a new small molecule are involved in a fungal-bacterial interaction
title_short	Phenotypic plasticity and a new small molecule are involved in a fungal-bacterial interaction
title_full	Phenotypic plasticity and a new small molecule are involved in a fungal-bacterial interaction
title_fullStr	Phenotypic plasticity and a new small molecule are involved in a fungal-bacterial interaction
title_full_unstemmed	Phenotypic plasticity and a new small molecule are involved in a fungal-bacterial interaction
title_sort	phenotypic plasticity and a new small molecule are involved in a fungal-bacterial interaction
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/09613681a6ad4f08a53555218badea54
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Phenotypic plasticity and a new small molecule are involved in a fungal-bacterial interaction

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