Re-inspection of small RNA sequence datasets reveals several novel human miRNA genes.

<h4>Background</h4>miRNAs are key players in gene expression regulation. To fully understand the complex nature of cellular differentiation or initiation and progression of disease, it is important to assess the expression patterns of as many miRNAs as possible. Thereby, identifying nove...

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Autores principales:	Thomas Birkballe Hansen, Jesper Bertram Bramsen, Jørgen Kjems
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0970e6c87a5e47db95db48565cc0af0a
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id	oai:doaj.org-article:0970e6c87a5e47db95db48565cc0af0a
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spelling	oai:doaj.org-article:0970e6c87a5e47db95db48565cc0af0a2021-12-02T20:21:10ZRe-inspection of small RNA sequence datasets reveals several novel human miRNA genes.1932-620310.1371/journal.pone.0010961https://doaj.org/article/0970e6c87a5e47db95db48565cc0af0a2010-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20532037/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>miRNAs are key players in gene expression regulation. To fully understand the complex nature of cellular differentiation or initiation and progression of disease, it is important to assess the expression patterns of as many miRNAs as possible. Thereby, identifying novel miRNAs is an essential prerequisite to make possible a comprehensive and coherent understanding of cellular biology.<h4>Methodology/principal findings</h4>Based on two extensive, but previously published, small RNA sequence datasets from human embryonic stem cells and human embroid bodies, respectively [1], we identified 112 novel miRNA-like structures and were able to validate miRNA processing in 12 out of 17 investigated cases. Several miRNA candidates were furthermore substantiated by including additional available small RNA datasets, thereby demonstrating the power of combining datasets to identify miRNAs that otherwise may be assigned as experimental noise.<h4>Conclusions/significance</h4>Our analysis highlights that existing datasets are not yet exhaustedly studied and continuous re-analysis of the available data is important to uncover all features of small RNA sequencing.Thomas Birkballe HansenJesper Bertram BramsenJørgen KjemsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 6, p e10961 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thomas Birkballe Hansen Jesper Bertram Bramsen Jørgen Kjems Re-inspection of small RNA sequence datasets reveals several novel human miRNA genes.
description	<h4>Background</h4>miRNAs are key players in gene expression regulation. To fully understand the complex nature of cellular differentiation or initiation and progression of disease, it is important to assess the expression patterns of as many miRNAs as possible. Thereby, identifying novel miRNAs is an essential prerequisite to make possible a comprehensive and coherent understanding of cellular biology.<h4>Methodology/principal findings</h4>Based on two extensive, but previously published, small RNA sequence datasets from human embryonic stem cells and human embroid bodies, respectively [1], we identified 112 novel miRNA-like structures and were able to validate miRNA processing in 12 out of 17 investigated cases. Several miRNA candidates were furthermore substantiated by including additional available small RNA datasets, thereby demonstrating the power of combining datasets to identify miRNAs that otherwise may be assigned as experimental noise.<h4>Conclusions/significance</h4>Our analysis highlights that existing datasets are not yet exhaustedly studied and continuous re-analysis of the available data is important to uncover all features of small RNA sequencing.
format	article
author	Thomas Birkballe Hansen Jesper Bertram Bramsen Jørgen Kjems
author_facet	Thomas Birkballe Hansen Jesper Bertram Bramsen Jørgen Kjems
author_sort	Thomas Birkballe Hansen
title	Re-inspection of small RNA sequence datasets reveals several novel human miRNA genes.
title_short	Re-inspection of small RNA sequence datasets reveals several novel human miRNA genes.
title_full	Re-inspection of small RNA sequence datasets reveals several novel human miRNA genes.
title_fullStr	Re-inspection of small RNA sequence datasets reveals several novel human miRNA genes.
title_full_unstemmed	Re-inspection of small RNA sequence datasets reveals several novel human miRNA genes.
title_sort	re-inspection of small rna sequence datasets reveals several novel human mirna genes.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/0970e6c87a5e47db95db48565cc0af0a
work_keys_str_mv	AT thomasbirkballehansen reinspectionofsmallrnasequencedatasetsrevealsseveralnovelhumanmirnagenes AT jesperbertrambramsen reinspectionofsmallrnasequencedatasetsrevealsseveralnovelhumanmirnagenes AT jørgenkjems reinspectionofsmallrnasequencedatasetsrevealsseveralnovelhumanmirnagenes
_version_	1718374148706664448

Re-inspection of small RNA sequence datasets reveals several novel human miRNA genes.

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