Embryonic miRNA profiles of normal and ectopic pregnancies.

Our objective was to investigate the miRNA profile of embryonic tissues in ectopic pregnancies (EPs) and controlled abortions (voluntary termination of pregnancy; VTOP). Twenty-three patients suffering from tubal EP and twenty-nine patients with a normal ongoing pregnancy scheduled for a VTOP were r...

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Autores principales:	Francisco Dominguez, Juan Manuel Moreno-Moya, Teresa Lozoya, Ainhoa Romero, Sebastian Martínez, Mercedes Monterde, Marta Gurrea, Blanca Ferri, Maria Jose Núñez, Carlos Simón, Antonio Pellicer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/09733bdb558e46f6b227170d227d1343
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spelling	oai:doaj.org-article:09733bdb558e46f6b227170d227d13432021-11-25T06:08:46ZEmbryonic miRNA profiles of normal and ectopic pregnancies.1932-620310.1371/journal.pone.0102185https://doaj.org/article/09733bdb558e46f6b227170d227d13432014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25013942/?tool=EBIhttps://doaj.org/toc/1932-6203Our objective was to investigate the miRNA profile of embryonic tissues in ectopic pregnancies (EPs) and controlled abortions (voluntary termination of pregnancy; VTOP). Twenty-three patients suffering from tubal EP and twenty-nine patients with a normal ongoing pregnancy scheduled for a VTOP were recruited. Embryonic tissue samples were analyzed by miRNA microarray and further validated by real time PCR. Microarray studies showed that four miRNAs were differentially downregulated (hsa-mir-196b, hsa-mir-30a, hsa-mir-873, and hsa-mir-337-3p) and three upregulated (hsa-mir-1288, hsa-mir-451, and hsa-mir-223) in EP compared to control tissue samples. Hsa-miR-196, hsa-miR-223, and hsa-miR-451 were further validated by real time PCR in a wider population of EP and control samples. We also performed a computational analysis to identify the gene targets and pathways which might be modulated by these three differentially expressed miRNAs. The most significant pathways found were the mucin type O-glycan biosynthesis and the ECM-receptor-interaction pathways. We also checked that the dysregulation of these three miRNAs was able to alter the expression of the gene targets in the embryonic tissues included in these pathways such as GALNT13 and ITGA2 genes. In conclusion, analysis of miRNAs in ectopic and eutopic embryonic tissues shows different expression patterns that could modify pathways which are critical for correct implantation, providing new insights into the understanding of ectopic implantation in humans.Francisco DominguezJuan Manuel Moreno-MoyaTeresa LozoyaAinhoa RomeroSebastian MartínezMercedes MonterdeMarta GurreaBlanca FerriMaria Jose NúñezCarlos SimónAntonio PellicerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e102185 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francisco Dominguez Juan Manuel Moreno-Moya Teresa Lozoya Ainhoa Romero Sebastian Martínez Mercedes Monterde Marta Gurrea Blanca Ferri Maria Jose Núñez Carlos Simón Antonio Pellicer Embryonic miRNA profiles of normal and ectopic pregnancies.
description	Our objective was to investigate the miRNA profile of embryonic tissues in ectopic pregnancies (EPs) and controlled abortions (voluntary termination of pregnancy; VTOP). Twenty-three patients suffering from tubal EP and twenty-nine patients with a normal ongoing pregnancy scheduled for a VTOP were recruited. Embryonic tissue samples were analyzed by miRNA microarray and further validated by real time PCR. Microarray studies showed that four miRNAs were differentially downregulated (hsa-mir-196b, hsa-mir-30a, hsa-mir-873, and hsa-mir-337-3p) and three upregulated (hsa-mir-1288, hsa-mir-451, and hsa-mir-223) in EP compared to control tissue samples. Hsa-miR-196, hsa-miR-223, and hsa-miR-451 were further validated by real time PCR in a wider population of EP and control samples. We also performed a computational analysis to identify the gene targets and pathways which might be modulated by these three differentially expressed miRNAs. The most significant pathways found were the mucin type O-glycan biosynthesis and the ECM-receptor-interaction pathways. We also checked that the dysregulation of these three miRNAs was able to alter the expression of the gene targets in the embryonic tissues included in these pathways such as GALNT13 and ITGA2 genes. In conclusion, analysis of miRNAs in ectopic and eutopic embryonic tissues shows different expression patterns that could modify pathways which are critical for correct implantation, providing new insights into the understanding of ectopic implantation in humans.
format	article
author	Francisco Dominguez Juan Manuel Moreno-Moya Teresa Lozoya Ainhoa Romero Sebastian Martínez Mercedes Monterde Marta Gurrea Blanca Ferri Maria Jose Núñez Carlos Simón Antonio Pellicer
author_facet	Francisco Dominguez Juan Manuel Moreno-Moya Teresa Lozoya Ainhoa Romero Sebastian Martínez Mercedes Monterde Marta Gurrea Blanca Ferri Maria Jose Núñez Carlos Simón Antonio Pellicer
author_sort	Francisco Dominguez
title	Embryonic miRNA profiles of normal and ectopic pregnancies.
title_short	Embryonic miRNA profiles of normal and ectopic pregnancies.
title_full	Embryonic miRNA profiles of normal and ectopic pregnancies.
title_fullStr	Embryonic miRNA profiles of normal and ectopic pregnancies.
title_full_unstemmed	Embryonic miRNA profiles of normal and ectopic pregnancies.
title_sort	embryonic mirna profiles of normal and ectopic pregnancies.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/09733bdb558e46f6b227170d227d1343
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_version_	1718414084240572416

Embryonic miRNA profiles of normal and ectopic pregnancies.

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