Induction of porcine host defense peptide gene expression by short-chain fatty acids and their analogs.

Dietary modulation of the synthesis of endogenous host defense peptides (HDPs) represents a novel antimicrobial approach for disease control and prevention, particularly against antibiotic-resistant infections. However, HDP regulation by dietary compounds such as butyrate is species-dependent. To ex...

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Autores principales:	Xiangfang Zeng, Lakshmi T Sunkara, Weiyu Jiang, Megan Bible, Scott Carter, Xi Ma, Shiyan Qiao, Guolong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/097667991a2c4cd0891070feb2f8247f
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spelling	oai:doaj.org-article:097667991a2c4cd0891070feb2f8247f2021-11-18T08:57:35ZInduction of porcine host defense peptide gene expression by short-chain fatty acids and their analogs.1932-620310.1371/journal.pone.0072922https://doaj.org/article/097667991a2c4cd0891070feb2f8247f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24023657/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Dietary modulation of the synthesis of endogenous host defense peptides (HDPs) represents a novel antimicrobial approach for disease control and prevention, particularly against antibiotic-resistant infections. However, HDP regulation by dietary compounds such as butyrate is species-dependent. To examine whether butyrate could induce HDP expression in pigs, we evaluated the expressions of a panel of porcine HDPs in IPEC-J2 intestinal epithelial cells, 3D4/31 macrophages, and primary monocytes in response to sodium butyrate treatment by real-time PCR. We revealed that butyrate is a potent inducer of multiple, but not all, HDP genes. Porcine β-defensin 2 (pBD2), pBD3, epididymis protein 2 splicing variant C (pEP2C), and protegrins were induced markedly in response to butyrate, whereas pBD1 expression remained largely unaltered in any cell type. Additionally, a comparison of the HDP-inducing efficacy among saturated free fatty acids of different aliphatic chain lengths revealed that fatty acids containing 3-8 carbons showed an obvious induction of HDP expression in IPEC-J2 cells, with butyrate being the most potent and long-chain fatty acids having only a marginal effect. We further investigated a panel of butyrate analogs for their efficacy in HDP induction, and found glyceryl tributyrate, benzyl butyrate, and 4-phenylbutyrate to be comparable with butyrate. Identification of butyrate and several analogs with a strong capacity to induce HDP gene expression in pigs provides attractive candidates for further evaluation of their potential as novel alternatives to antibiotics in augmenting innate immunity and disease resistance of pigs.Xiangfang ZengLakshmi T SunkaraWeiyu JiangMegan BibleScott CarterXi MaShiyan QiaoGuolong ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e72922 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiangfang Zeng Lakshmi T Sunkara Weiyu Jiang Megan Bible Scott Carter Xi Ma Shiyan Qiao Guolong Zhang Induction of porcine host defense peptide gene expression by short-chain fatty acids and their analogs.
description	Dietary modulation of the synthesis of endogenous host defense peptides (HDPs) represents a novel antimicrobial approach for disease control and prevention, particularly against antibiotic-resistant infections. However, HDP regulation by dietary compounds such as butyrate is species-dependent. To examine whether butyrate could induce HDP expression in pigs, we evaluated the expressions of a panel of porcine HDPs in IPEC-J2 intestinal epithelial cells, 3D4/31 macrophages, and primary monocytes in response to sodium butyrate treatment by real-time PCR. We revealed that butyrate is a potent inducer of multiple, but not all, HDP genes. Porcine β-defensin 2 (pBD2), pBD3, epididymis protein 2 splicing variant C (pEP2C), and protegrins were induced markedly in response to butyrate, whereas pBD1 expression remained largely unaltered in any cell type. Additionally, a comparison of the HDP-inducing efficacy among saturated free fatty acids of different aliphatic chain lengths revealed that fatty acids containing 3-8 carbons showed an obvious induction of HDP expression in IPEC-J2 cells, with butyrate being the most potent and long-chain fatty acids having only a marginal effect. We further investigated a panel of butyrate analogs for their efficacy in HDP induction, and found glyceryl tributyrate, benzyl butyrate, and 4-phenylbutyrate to be comparable with butyrate. Identification of butyrate and several analogs with a strong capacity to induce HDP gene expression in pigs provides attractive candidates for further evaluation of their potential as novel alternatives to antibiotics in augmenting innate immunity and disease resistance of pigs.
format	article
author	Xiangfang Zeng Lakshmi T Sunkara Weiyu Jiang Megan Bible Scott Carter Xi Ma Shiyan Qiao Guolong Zhang
author_facet	Xiangfang Zeng Lakshmi T Sunkara Weiyu Jiang Megan Bible Scott Carter Xi Ma Shiyan Qiao Guolong Zhang
author_sort	Xiangfang Zeng
title	Induction of porcine host defense peptide gene expression by short-chain fatty acids and their analogs.
title_short	Induction of porcine host defense peptide gene expression by short-chain fatty acids and their analogs.
title_full	Induction of porcine host defense peptide gene expression by short-chain fatty acids and their analogs.
title_fullStr	Induction of porcine host defense peptide gene expression by short-chain fatty acids and their analogs.
title_full_unstemmed	Induction of porcine host defense peptide gene expression by short-chain fatty acids and their analogs.
title_sort	induction of porcine host defense peptide gene expression by short-chain fatty acids and their analogs.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/097667991a2c4cd0891070feb2f8247f
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Induction of porcine host defense peptide gene expression by short-chain fatty acids and their analogs.

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