Endogenous TasiRNAs mediate non-cell autonomous effects on gene regulation in Arabidopsis thaliana.
<h4>Background</h4>Different classes of small RNAs (sRNAs) refine the expression of numerous genes in higher eukaryotes by directing protein partners to complementary nucleic acids, where they mediate gene silencing. Plants encode a unique class of sRNAs, called trans-acting small interf...
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2009
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oai:doaj.org-article:097b1feded9b42619ca784b33b2828a52021-11-25T06:22:01ZEndogenous TasiRNAs mediate non-cell autonomous effects on gene regulation in Arabidopsis thaliana.1932-620310.1371/journal.pone.0005980https://doaj.org/article/097b1feded9b42619ca784b33b2828a52009-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19543387/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Different classes of small RNAs (sRNAs) refine the expression of numerous genes in higher eukaryotes by directing protein partners to complementary nucleic acids, where they mediate gene silencing. Plants encode a unique class of sRNAs, called trans-acting small interfering RNAs (tasiRNAs), which post-transcriptionally regulate protein-coding transcripts, as do microRNAs (miRNAs), and both sRNA classes control development through their targets. TasiRNA biogenesis requires multiple components of the siRNA pathway and also miRNAs. But while 21mer siRNAs originating from transgenes can mediate silencing across several cell layers, miRNA action seems spatially restricted to the producing or closely surrounding cells.<h4>Principal findings</h4>We have previously described the isolation of a genetrap reporter line for TAS3a, the major locus producing AUXIN RESPONS FACTOR (ARF)-regulating tasiRNAs in the Arabidopsis shoot. Its activity is limited to the adaxial (upper) side of leaf primordia, thus spatially isolated from ARF-activities, which are located in the abaxial (lower) side. We show here by in situ hybridization and reporter fusions that the silencing activities of ARF-regulating tasiRNAs are indeed manifested non-cell autonomously to spatially control ARF activities.<h4>Conclusions/significance</h4>Endogenous tasiRNAs are thus mediators of a mobile developmental signal and might provide effective gene silencing at a distance beyond the reach of most miRNAs.Rebecca SchwabAlexis MaizelVirginia Ruiz-FerrerDamien GarciaMartin BayerMartin CrespiOlivier VoinnetRobert A MartienssenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 6, p e5980 (2009) |
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Medicine R Science Q Rebecca Schwab Alexis Maizel Virginia Ruiz-Ferrer Damien Garcia Martin Bayer Martin Crespi Olivier Voinnet Robert A Martienssen Endogenous TasiRNAs mediate non-cell autonomous effects on gene regulation in Arabidopsis thaliana. |
| description |
<h4>Background</h4>Different classes of small RNAs (sRNAs) refine the expression of numerous genes in higher eukaryotes by directing protein partners to complementary nucleic acids, where they mediate gene silencing. Plants encode a unique class of sRNAs, called trans-acting small interfering RNAs (tasiRNAs), which post-transcriptionally regulate protein-coding transcripts, as do microRNAs (miRNAs), and both sRNA classes control development through their targets. TasiRNA biogenesis requires multiple components of the siRNA pathway and also miRNAs. But while 21mer siRNAs originating from transgenes can mediate silencing across several cell layers, miRNA action seems spatially restricted to the producing or closely surrounding cells.<h4>Principal findings</h4>We have previously described the isolation of a genetrap reporter line for TAS3a, the major locus producing AUXIN RESPONS FACTOR (ARF)-regulating tasiRNAs in the Arabidopsis shoot. Its activity is limited to the adaxial (upper) side of leaf primordia, thus spatially isolated from ARF-activities, which are located in the abaxial (lower) side. We show here by in situ hybridization and reporter fusions that the silencing activities of ARF-regulating tasiRNAs are indeed manifested non-cell autonomously to spatially control ARF activities.<h4>Conclusions/significance</h4>Endogenous tasiRNAs are thus mediators of a mobile developmental signal and might provide effective gene silencing at a distance beyond the reach of most miRNAs. |
| format |
article |
| author |
Rebecca Schwab Alexis Maizel Virginia Ruiz-Ferrer Damien Garcia Martin Bayer Martin Crespi Olivier Voinnet Robert A Martienssen |
| author_facet |
Rebecca Schwab Alexis Maizel Virginia Ruiz-Ferrer Damien Garcia Martin Bayer Martin Crespi Olivier Voinnet Robert A Martienssen |
| author_sort |
Rebecca Schwab |
| title |
Endogenous TasiRNAs mediate non-cell autonomous effects on gene regulation in Arabidopsis thaliana. |
| title_short |
Endogenous TasiRNAs mediate non-cell autonomous effects on gene regulation in Arabidopsis thaliana. |
| title_full |
Endogenous TasiRNAs mediate non-cell autonomous effects on gene regulation in Arabidopsis thaliana. |
| title_fullStr |
Endogenous TasiRNAs mediate non-cell autonomous effects on gene regulation in Arabidopsis thaliana. |
| title_full_unstemmed |
Endogenous TasiRNAs mediate non-cell autonomous effects on gene regulation in Arabidopsis thaliana. |
| title_sort |
endogenous tasirnas mediate non-cell autonomous effects on gene regulation in arabidopsis thaliana. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2009 |
| url |
https://doaj.org/article/097b1feded9b42619ca784b33b2828a5 |
| work_keys_str_mv |
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| _version_ |
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