Sequencing and characterisation of rearrangements in three S. pastorianus strains reveals the presence of chimeric genes and gives evidence of breakpoint reuse.

Gross chromosomal rearrangements have the potential to be evolutionarily advantageous to an adapting organism. The generation of a hybrid species increases opportunity for recombination by bringing together two homologous genomes. We sought to define the location of genomic rearrangements in three s...

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Autores principales:	Sarah K Hewitt, Ian J Donaldson, Simon C Lovell, Daniela Delneri
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/097d6bfa6fca41b99c027ce3588d017b
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spelling	oai:doaj.org-article:097d6bfa6fca41b99c027ce3588d017b2021-11-18T08:27:32ZSequencing and characterisation of rearrangements in three S. pastorianus strains reveals the presence of chimeric genes and gives evidence of breakpoint reuse.1932-620310.1371/journal.pone.0092203https://doaj.org/article/097d6bfa6fca41b99c027ce3588d017b2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24643015/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Gross chromosomal rearrangements have the potential to be evolutionarily advantageous to an adapting organism. The generation of a hybrid species increases opportunity for recombination by bringing together two homologous genomes. We sought to define the location of genomic rearrangements in three strains of Saccharomyces pastorianus, a natural lager-brewing yeast hybrid of Saccharomyces cerevisiae and Saccharomyces eubayanus, using whole genome shotgun sequencing. Each strain of S. pastorianus has lost species-specific portions of its genome and has undergone extensive recombination, producing chimeric chromosomes. We predicted 30 breakpoints that we confirmed at the single nucleotide level by designing species-specific primers that flank each breakpoint, and then sequencing the PCR product. These rearrangements are the result of recombination between areas of homology between the two subgenomes, rather than repetitive elements such as transposons or tRNAs. Interestingly, 28/30 S. cerevisiae-S. eubayanus recombination breakpoints are located within genic regions, generating chimeric genes. Furthermore we show evidence for the reuse of two breakpoints, located in HSP82 and KEM1, in strains of proposed independent origin.Sarah K HewittIan J DonaldsonSimon C LovellDaniela DelneriPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e92203 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sarah K Hewitt Ian J Donaldson Simon C Lovell Daniela Delneri Sequencing and characterisation of rearrangements in three S. pastorianus strains reveals the presence of chimeric genes and gives evidence of breakpoint reuse.
description	Gross chromosomal rearrangements have the potential to be evolutionarily advantageous to an adapting organism. The generation of a hybrid species increases opportunity for recombination by bringing together two homologous genomes. We sought to define the location of genomic rearrangements in three strains of Saccharomyces pastorianus, a natural lager-brewing yeast hybrid of Saccharomyces cerevisiae and Saccharomyces eubayanus, using whole genome shotgun sequencing. Each strain of S. pastorianus has lost species-specific portions of its genome and has undergone extensive recombination, producing chimeric chromosomes. We predicted 30 breakpoints that we confirmed at the single nucleotide level by designing species-specific primers that flank each breakpoint, and then sequencing the PCR product. These rearrangements are the result of recombination between areas of homology between the two subgenomes, rather than repetitive elements such as transposons or tRNAs. Interestingly, 28/30 S. cerevisiae-S. eubayanus recombination breakpoints are located within genic regions, generating chimeric genes. Furthermore we show evidence for the reuse of two breakpoints, located in HSP82 and KEM1, in strains of proposed independent origin.
format	article
author	Sarah K Hewitt Ian J Donaldson Simon C Lovell Daniela Delneri
author_facet	Sarah K Hewitt Ian J Donaldson Simon C Lovell Daniela Delneri
author_sort	Sarah K Hewitt
title	Sequencing and characterisation of rearrangements in three S. pastorianus strains reveals the presence of chimeric genes and gives evidence of breakpoint reuse.
title_short	Sequencing and characterisation of rearrangements in three S. pastorianus strains reveals the presence of chimeric genes and gives evidence of breakpoint reuse.
title_full	Sequencing and characterisation of rearrangements in three S. pastorianus strains reveals the presence of chimeric genes and gives evidence of breakpoint reuse.
title_fullStr	Sequencing and characterisation of rearrangements in three S. pastorianus strains reveals the presence of chimeric genes and gives evidence of breakpoint reuse.
title_full_unstemmed	Sequencing and characterisation of rearrangements in three S. pastorianus strains reveals the presence of chimeric genes and gives evidence of breakpoint reuse.
title_sort	sequencing and characterisation of rearrangements in three s. pastorianus strains reveals the presence of chimeric genes and gives evidence of breakpoint reuse.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/097d6bfa6fca41b99c027ce3588d017b
work_keys_str_mv	AT sarahkhewitt sequencingandcharacterisationofrearrangementsinthreespastorianusstrainsrevealsthepresenceofchimericgenesandgivesevidenceofbreakpointreuse AT ianjdonaldson sequencingandcharacterisationofrearrangementsinthreespastorianusstrainsrevealsthepresenceofchimericgenesandgivesevidenceofbreakpointreuse AT simonclovell sequencingandcharacterisationofrearrangementsinthreespastorianusstrainsrevealsthepresenceofchimericgenesandgivesevidenceofbreakpointreuse AT danieladelneri sequencingandcharacterisationofrearrangementsinthreespastorianusstrainsrevealsthepresenceofchimericgenesandgivesevidenceofbreakpointreuse
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Sequencing and characterisation of rearrangements in three S. pastorianus strains reveals the presence of chimeric genes and gives evidence of breakpoint reuse.

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