Effects of 45S5 bioactive glass on the remineralization of early carious lesions in deciduous teeth: an in vitro study

Abstract Background Early childhood caries has been designated as a serious public health problem. The traditional restoration method is very challenging, especially in uncooperative patients. Non-invasive therapy, like remineralization agents, which have been developed to reverse the demineralizati...

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Autores principales:	Rui Zhang, Jianyan Qi, Min Gong, Qian liu, Hongyan Zhou, Jue Wang, Yufeng Mei
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Bioactive glass Remineralization Deciduous teeth Energy dispersive X-ray spectroscopy Fourier transform infrared spectroscopy equipped with attenuated total reflectance Dentistry RK1-715
Acceso en línea:	https://doaj.org/article/097e00c0fbc34af18a90e2f705f1ba99
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spelling	oai:doaj.org-article:097e00c0fbc34af18a90e2f705f1ba992021-11-14T12:32:08ZEffects of 45S5 bioactive glass on the remineralization of early carious lesions in deciduous teeth: an in vitro study10.1186/s12903-021-01931-31472-6831https://doaj.org/article/097e00c0fbc34af18a90e2f705f1ba992021-11-01T00:00:00Zhttps://doi.org/10.1186/s12903-021-01931-3https://doaj.org/toc/1472-6831Abstract Background Early childhood caries has been designated as a serious public health problem. The traditional restoration method is very challenging, especially in uncooperative patients. Non-invasive therapy, like remineralization agents, which have been developed to reverse the demineralization progress at the early stage of caries, may be a better choice. This study aimed to evaluate the remineralization efficacy of different concentrations of 45S5 bioactive glass (BAG) on artifical carious lesions of deciduous enamel. Methods 65 caries-like enamel lesions of the deciduous teeth were assigned to 5 groups (n = 13) and transported to a 14 days pH-cycling: Group A: 2%BAG, Group B: 4%BAG, Group C: 6%BAG, Group D: 8%BAG, and Group E: deionized water (DDW, negative control). 8 sound (Group F) and 8 demineralized teeth (Group G) were prepared for contrast. The recovery power of mechanical property was evaluated by Vickers hardness test through the recovery of enamel microhardness (%REMH). Surface morphology, mass fraction of Ca and P ions, and Ca/P atomic ratio were analyzed by scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (EDX). Moreover, Fourier transform infrared spectroscopy equipped with attenuated total reflectance was used to identify the chemical structure of newly formed compounds. Results % REMH were (42.65 ± 1.35), (52.59 ± 2.96), (57.40 ± 1.72), (52.91 ± 2.55), (12.46 ± 2.81) in 2%BAG, 4%BAG, 6%BAG, 8%BAG, and DDW groups respectively. Micro-spherical particles were deposited in all BAG groups and 6% BAG showed the densest and most uniform surface. EDX analysis identified significantly higher Ca(wt%) and P(wt%) in four BAG groups than in the demineralized group (p < 0.005), while 6% BAG showed the highest mineral gain efficacy. The infrared spectrum demonstrated that newly mineralized crystals were consisted of type-B hydroxycarbonate apatite. Conclusion BAG possessed a promising remineralization effect on artificial lesions in deciduous enamel by recovering enamel surface mechanical property, morphology and chemical elements. Among them, 6% BAG performed the greatest overall efficacy. Acting as a new caries-arresting biomaterial, 45S5 BAG has the potential to facilitate the adaptation of better carious prevention strategies in children.Rui ZhangJianyan QiMin GongQian liuHongyan ZhouJue WangYufeng MeiBMCarticleBioactive glassRemineralizationDeciduous teethEnergy dispersive X-ray spectroscopyFourier transform infrared spectroscopy equipped with attenuated total reflectanceDentistryRK1-715ENBMC Oral Health, Vol 21, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bioactive glass Remineralization Deciduous teeth Energy dispersive X-ray spectroscopy Fourier transform infrared spectroscopy equipped with attenuated total reflectance Dentistry RK1-715
spellingShingle	Bioactive glass Remineralization Deciduous teeth Energy dispersive X-ray spectroscopy Fourier transform infrared spectroscopy equipped with attenuated total reflectance Dentistry RK1-715 Rui Zhang Jianyan Qi Min Gong Qian liu Hongyan Zhou Jue Wang Yufeng Mei Effects of 45S5 bioactive glass on the remineralization of early carious lesions in deciduous teeth: an in vitro study
description	Abstract Background Early childhood caries has been designated as a serious public health problem. The traditional restoration method is very challenging, especially in uncooperative patients. Non-invasive therapy, like remineralization agents, which have been developed to reverse the demineralization progress at the early stage of caries, may be a better choice. This study aimed to evaluate the remineralization efficacy of different concentrations of 45S5 bioactive glass (BAG) on artifical carious lesions of deciduous enamel. Methods 65 caries-like enamel lesions of the deciduous teeth were assigned to 5 groups (n = 13) and transported to a 14 days pH-cycling: Group A: 2%BAG, Group B: 4%BAG, Group C: 6%BAG, Group D: 8%BAG, and Group E: deionized water (DDW, negative control). 8 sound (Group F) and 8 demineralized teeth (Group G) were prepared for contrast. The recovery power of mechanical property was evaluated by Vickers hardness test through the recovery of enamel microhardness (%REMH). Surface morphology, mass fraction of Ca and P ions, and Ca/P atomic ratio were analyzed by scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (EDX). Moreover, Fourier transform infrared spectroscopy equipped with attenuated total reflectance was used to identify the chemical structure of newly formed compounds. Results % REMH were (42.65 ± 1.35), (52.59 ± 2.96), (57.40 ± 1.72), (52.91 ± 2.55), (12.46 ± 2.81) in 2%BAG, 4%BAG, 6%BAG, 8%BAG, and DDW groups respectively. Micro-spherical particles were deposited in all BAG groups and 6% BAG showed the densest and most uniform surface. EDX analysis identified significantly higher Ca(wt%) and P(wt%) in four BAG groups than in the demineralized group (p < 0.005), while 6% BAG showed the highest mineral gain efficacy. The infrared spectrum demonstrated that newly mineralized crystals were consisted of type-B hydroxycarbonate apatite. Conclusion BAG possessed a promising remineralization effect on artificial lesions in deciduous enamel by recovering enamel surface mechanical property, morphology and chemical elements. Among them, 6% BAG performed the greatest overall efficacy. Acting as a new caries-arresting biomaterial, 45S5 BAG has the potential to facilitate the adaptation of better carious prevention strategies in children.
format	article
author	Rui Zhang Jianyan Qi Min Gong Qian liu Hongyan Zhou Jue Wang Yufeng Mei
author_facet	Rui Zhang Jianyan Qi Min Gong Qian liu Hongyan Zhou Jue Wang Yufeng Mei
author_sort	Rui Zhang
title	Effects of 45S5 bioactive glass on the remineralization of early carious lesions in deciduous teeth: an in vitro study
title_short	Effects of 45S5 bioactive glass on the remineralization of early carious lesions in deciduous teeth: an in vitro study
title_full	Effects of 45S5 bioactive glass on the remineralization of early carious lesions in deciduous teeth: an in vitro study
title_fullStr	Effects of 45S5 bioactive glass on the remineralization of early carious lesions in deciduous teeth: an in vitro study
title_full_unstemmed	Effects of 45S5 bioactive glass on the remineralization of early carious lesions in deciduous teeth: an in vitro study
title_sort	effects of 45s5 bioactive glass on the remineralization of early carious lesions in deciduous teeth: an in vitro study
publisher	BMC
publishDate	2021
url	https://doaj.org/article/097e00c0fbc34af18a90e2f705f1ba99
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Effects of 45S5 bioactive glass on the remineralization of early carious lesions in deciduous teeth: an in vitro study

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