A Microfluidic Aptamer-Based Sensor for Detection of Mercury(II) and Lead(II) Ions in Water

Heavy metal contaminants have serious consequences for the environment and human health. Consequently, effective methods for detecting their presence, particularly in water and food, are urgently required. Accordingly, the present study proposes a sensor capable of detecting mercury Hg(II) and lead...

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Autores principales:	Wei-Hao Huang, Van-Phung Mai, Ruo-Yin Wu, Ko-Li Yeh, Ruey-Jen Yang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	aptamer fluorescence resonance energy transfer heavy metal ions graphene oxide microfluidic device sensor Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/0982dbc5984a4785beeae3ceb38a1f07
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spelling	oai:doaj.org-article:0982dbc5984a4785beeae3ceb38a1f072021-11-25T18:22:46ZA Microfluidic Aptamer-Based Sensor for Detection of Mercury(II) and Lead(II) Ions in Water10.3390/mi121112832072-666Xhttps://doaj.org/article/0982dbc5984a4785beeae3ceb38a1f072021-10-01T00:00:00Zhttps://www.mdpi.com/2072-666X/12/11/1283https://doaj.org/toc/2072-666XHeavy metal contaminants have serious consequences for the environment and human health. Consequently, effective methods for detecting their presence, particularly in water and food, are urgently required. Accordingly, the present study proposes a sensor capable of detecting mercury Hg(II) and lead Pb(II) ions simultaneously, using graphene oxide (GO) as a quenching agent and an aptamer solution as a reagent. In the proposed device, the aptamer sequences are labeled by FAM and HEX fluorescent dyes, respectively, and are mixed well with 500 ppm GO solution before injection into one inlet of the microchannel, and the heavy metal sample solution is injected into another inlet. The presence of Hg(II) and Pb(II) ions is then detected by measuring the change in the fluorescence intensity of the GO/aptamer suspension as the aptamer molecules undergo fluorescence resonance energy transfer (FRET). The selectivity of these two ions is also shown to be clear among other mixed heavy metal ions. The experimental results show that the aptamer sensors have a linear range of 10~250 nM (i.e., 2.0~50 ppb) for Hg(II) ions and 10~100 nM (i.e., 2.1~20.7 ppb) for Pb(II) ions. Furthermore, the limit of detection is around 0.70 ppb and 0.53 ppb for Hg(II) and Pb(II), respectively, which is lower than the maximum limits of 6 ppb and 10 ppb prescribed by the World Health Organization (WHO) for Hg(II) and Pb(II) in drinking water, respectively.Wei-Hao HuangVan-Phung MaiRuo-Yin WuKo-Li YehRuey-Jen YangMDPI AGarticleaptamerfluorescence resonance energy transferheavy metal ionsgraphene oxidemicrofluidic devicesensorMechanical engineering and machineryTJ1-1570ENMicromachines, Vol 12, Iss 1283, p 1283 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aptamer fluorescence resonance energy transfer heavy metal ions graphene oxide microfluidic device sensor Mechanical engineering and machinery TJ1-1570
spellingShingle	aptamer fluorescence resonance energy transfer heavy metal ions graphene oxide microfluidic device sensor Mechanical engineering and machinery TJ1-1570 Wei-Hao Huang Van-Phung Mai Ruo-Yin Wu Ko-Li Yeh Ruey-Jen Yang A Microfluidic Aptamer-Based Sensor for Detection of Mercury(II) and Lead(II) Ions in Water
description	Heavy metal contaminants have serious consequences for the environment and human health. Consequently, effective methods for detecting their presence, particularly in water and food, are urgently required. Accordingly, the present study proposes a sensor capable of detecting mercury Hg(II) and lead Pb(II) ions simultaneously, using graphene oxide (GO) as a quenching agent and an aptamer solution as a reagent. In the proposed device, the aptamer sequences are labeled by FAM and HEX fluorescent dyes, respectively, and are mixed well with 500 ppm GO solution before injection into one inlet of the microchannel, and the heavy metal sample solution is injected into another inlet. The presence of Hg(II) and Pb(II) ions is then detected by measuring the change in the fluorescence intensity of the GO/aptamer suspension as the aptamer molecules undergo fluorescence resonance energy transfer (FRET). The selectivity of these two ions is also shown to be clear among other mixed heavy metal ions. The experimental results show that the aptamer sensors have a linear range of 10~250 nM (i.e., 2.0~50 ppb) for Hg(II) ions and 10~100 nM (i.e., 2.1~20.7 ppb) for Pb(II) ions. Furthermore, the limit of detection is around 0.70 ppb and 0.53 ppb for Hg(II) and Pb(II), respectively, which is lower than the maximum limits of 6 ppb and 10 ppb prescribed by the World Health Organization (WHO) for Hg(II) and Pb(II) in drinking water, respectively.
format	article
author	Wei-Hao Huang Van-Phung Mai Ruo-Yin Wu Ko-Li Yeh Ruey-Jen Yang
author_facet	Wei-Hao Huang Van-Phung Mai Ruo-Yin Wu Ko-Li Yeh Ruey-Jen Yang
author_sort	Wei-Hao Huang
title	A Microfluidic Aptamer-Based Sensor for Detection of Mercury(II) and Lead(II) Ions in Water
title_short	A Microfluidic Aptamer-Based Sensor for Detection of Mercury(II) and Lead(II) Ions in Water
title_full	A Microfluidic Aptamer-Based Sensor for Detection of Mercury(II) and Lead(II) Ions in Water
title_fullStr	A Microfluidic Aptamer-Based Sensor for Detection of Mercury(II) and Lead(II) Ions in Water
title_full_unstemmed	A Microfluidic Aptamer-Based Sensor for Detection of Mercury(II) and Lead(II) Ions in Water
title_sort	microfluidic aptamer-based sensor for detection of mercury(ii) and lead(ii) ions in water
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0982dbc5984a4785beeae3ceb38a1f07
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A Microfluidic Aptamer-Based Sensor for Detection of Mercury(II) and Lead(II) Ions in Water

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