Plasma microRNA profiles in rat models of hepatocellular injury, cholestasis, and steatosis.

MicroRNAs (miRNAs) are small RNA molecules that function to modulate the expression of target genes, playing important roles in a wide range of physiological and pathological processes. The miRNAs in body fluids have received considerable attention as potential biomarkers of various diseases. In thi...

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Autores principales:	Yu Yamaura, Miki Nakajima, Shingo Takagi, Tatsuki Fukami, Koichi Tsuneyama, Tsuyoshi Yokoi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0984a55d289d4135b90de3377be46fc1
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spelling	oai:doaj.org-article:0984a55d289d4135b90de3377be46fc12021-11-18T07:27:49ZPlasma microRNA profiles in rat models of hepatocellular injury, cholestasis, and steatosis.1932-620310.1371/journal.pone.0030250https://doaj.org/article/0984a55d289d4135b90de3377be46fc12012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22363424/?tool=EBIhttps://doaj.org/toc/1932-6203MicroRNAs (miRNAs) are small RNA molecules that function to modulate the expression of target genes, playing important roles in a wide range of physiological and pathological processes. The miRNAs in body fluids have received considerable attention as potential biomarkers of various diseases. In this study, we compared the changes of the plasma miRNA expressions by acute liver injury (hepatocellular injury or cholestasis) and chronic liver injury (steatosis, steatohepatitis and fibrosis) using rat models made by the administration of chemicals or special diets. Using miRNA array analysis, we found that the levels of a large number of miRNAs (121-317 miRNAs) were increased over 2-fold and the levels of a small number of miRNAs (6-35 miRNAs) were decreased below 0.5-fold in all models except in a model of cholestasis caused by bile duct ligation. Interestingly, the expression profiles were different between the models, and the hierarchical clustering analysis discriminated between the acute and chronic liver injuries. In addition, miRNAs whose expressions were typically changed in each type of liver injury could be specified. It is notable that, in acute liver injury models, the plasma level of miR-122, the most abundant miRNA in the liver, was more quickly and dramatically increased than the plasma aminotransferase level, reflecting the extent of hepatocellular injury. This study demonstrated that the plasma miRNA profiles could reflect the types of liver injury (e.g. acute/chronic liver injury or hepatocellular injury/cholestasis/steatosis/steatohepatitis/fibrosis) and identified the miRNAs that could be specific and sensitive biomarkers of liver injury.Yu YamauraMiki NakajimaShingo TakagiTatsuki FukamiKoichi TsuneyamaTsuyoshi YokoiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e30250 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yu Yamaura Miki Nakajima Shingo Takagi Tatsuki Fukami Koichi Tsuneyama Tsuyoshi Yokoi Plasma microRNA profiles in rat models of hepatocellular injury, cholestasis, and steatosis.
description	MicroRNAs (miRNAs) are small RNA molecules that function to modulate the expression of target genes, playing important roles in a wide range of physiological and pathological processes. The miRNAs in body fluids have received considerable attention as potential biomarkers of various diseases. In this study, we compared the changes of the plasma miRNA expressions by acute liver injury (hepatocellular injury or cholestasis) and chronic liver injury (steatosis, steatohepatitis and fibrosis) using rat models made by the administration of chemicals or special diets. Using miRNA array analysis, we found that the levels of a large number of miRNAs (121-317 miRNAs) were increased over 2-fold and the levels of a small number of miRNAs (6-35 miRNAs) were decreased below 0.5-fold in all models except in a model of cholestasis caused by bile duct ligation. Interestingly, the expression profiles were different between the models, and the hierarchical clustering analysis discriminated between the acute and chronic liver injuries. In addition, miRNAs whose expressions were typically changed in each type of liver injury could be specified. It is notable that, in acute liver injury models, the plasma level of miR-122, the most abundant miRNA in the liver, was more quickly and dramatically increased than the plasma aminotransferase level, reflecting the extent of hepatocellular injury. This study demonstrated that the plasma miRNA profiles could reflect the types of liver injury (e.g. acute/chronic liver injury or hepatocellular injury/cholestasis/steatosis/steatohepatitis/fibrosis) and identified the miRNAs that could be specific and sensitive biomarkers of liver injury.
format	article
author	Yu Yamaura Miki Nakajima Shingo Takagi Tatsuki Fukami Koichi Tsuneyama Tsuyoshi Yokoi
author_facet	Yu Yamaura Miki Nakajima Shingo Takagi Tatsuki Fukami Koichi Tsuneyama Tsuyoshi Yokoi
author_sort	Yu Yamaura
title	Plasma microRNA profiles in rat models of hepatocellular injury, cholestasis, and steatosis.
title_short	Plasma microRNA profiles in rat models of hepatocellular injury, cholestasis, and steatosis.
title_full	Plasma microRNA profiles in rat models of hepatocellular injury, cholestasis, and steatosis.
title_fullStr	Plasma microRNA profiles in rat models of hepatocellular injury, cholestasis, and steatosis.
title_full_unstemmed	Plasma microRNA profiles in rat models of hepatocellular injury, cholestasis, and steatosis.
title_sort	plasma microrna profiles in rat models of hepatocellular injury, cholestasis, and steatosis.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/0984a55d289d4135b90de3377be46fc1
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Plasma microRNA profiles in rat models of hepatocellular injury, cholestasis, and steatosis.

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