Gene expression and morphological responses of Lolium perenne L. exposed to cadmium (Cd2+) and mercury (Hg2+)

Abstract Soil contamination with heavy metals is a major problem worldwide, due to the increasing impact mainly caused by anthropogenic activities. This research evaluated the phytoremediation capacity of, Lolium perenne for heavy metals such as cadmium (Cd2+) and mercury (Hg2+), and the effects of...

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Autores principales:	Yuby Cruz, Sharik Villar, Karen Gutiérrez, Carolina Montoya-Ruiz, Jorge L. Gallego, Maria del Pilar Delgado, Juan F. Saldarriaga
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/098859ec5c2b4778adc33acfdd592b27
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spelling	oai:doaj.org-article:098859ec5c2b4778adc33acfdd592b272021-12-02T15:00:14ZGene expression and morphological responses of Lolium perenne L. exposed to cadmium (Cd2+) and mercury (Hg2+)10.1038/s41598-021-90826-y2045-2322https://doaj.org/article/098859ec5c2b4778adc33acfdd592b272021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90826-yhttps://doaj.org/toc/2045-2322Abstract Soil contamination with heavy metals is a major problem worldwide, due to the increasing impact mainly caused by anthropogenic activities. This research evaluated the phytoremediation capacity of, Lolium perenne for heavy metals such as cadmium (Cd2+) and mercury (Hg2+), and the effects of these metals on morphology, biomass production, and the changes on gene expression. Seeds of L. perenne were exposed to six concentrations of Cd2+ and Hg2+ in the range of 0 to 25 mg L−1, and two mixtures of Cd2+–Hg2. The Non-Observed Effect Level (NOEL) was established with dose response curves and the expression of specific genes was evaluated applying a commercially available quantitative reverse transcription (RT-qPCR) assay. There was no significant effect when exposing the seeds to Hg2+, for Cd2+ the maximum concentration was established in 0.1 mg L−1, and for the two concentrations of mixtures, there was a negative effect. An increase of expression of genes that regulate antioxidant activity and stress was found when the plant was exposed to heavy metals. Given the high tolerance to metals analyzed that was reflected both, the development of the plant and in its molecular response, these results highlight that L. perenne is a plant with phytoremediator potential.Yuby CruzSharik VillarKaren GutiérrezCarolina Montoya-RuizJorge L. GallegoMaria del Pilar DelgadoJuan F. SaldarriagaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuby Cruz Sharik Villar Karen Gutiérrez Carolina Montoya-Ruiz Jorge L. Gallego Maria del Pilar Delgado Juan F. Saldarriaga Gene expression and morphological responses of Lolium perenne L. exposed to cadmium (Cd2+) and mercury (Hg2+)
description	Abstract Soil contamination with heavy metals is a major problem worldwide, due to the increasing impact mainly caused by anthropogenic activities. This research evaluated the phytoremediation capacity of, Lolium perenne for heavy metals such as cadmium (Cd2+) and mercury (Hg2+), and the effects of these metals on morphology, biomass production, and the changes on gene expression. Seeds of L. perenne were exposed to six concentrations of Cd2+ and Hg2+ in the range of 0 to 25 mg L−1, and two mixtures of Cd2+–Hg2. The Non-Observed Effect Level (NOEL) was established with dose response curves and the expression of specific genes was evaluated applying a commercially available quantitative reverse transcription (RT-qPCR) assay. There was no significant effect when exposing the seeds to Hg2+, for Cd2+ the maximum concentration was established in 0.1 mg L−1, and for the two concentrations of mixtures, there was a negative effect. An increase of expression of genes that regulate antioxidant activity and stress was found when the plant was exposed to heavy metals. Given the high tolerance to metals analyzed that was reflected both, the development of the plant and in its molecular response, these results highlight that L. perenne is a plant with phytoremediator potential.
format	article
author	Yuby Cruz Sharik Villar Karen Gutiérrez Carolina Montoya-Ruiz Jorge L. Gallego Maria del Pilar Delgado Juan F. Saldarriaga
author_facet	Yuby Cruz Sharik Villar Karen Gutiérrez Carolina Montoya-Ruiz Jorge L. Gallego Maria del Pilar Delgado Juan F. Saldarriaga
author_sort	Yuby Cruz
title	Gene expression and morphological responses of Lolium perenne L. exposed to cadmium (Cd2+) and mercury (Hg2+)
title_short	Gene expression and morphological responses of Lolium perenne L. exposed to cadmium (Cd2+) and mercury (Hg2+)
title_full	Gene expression and morphological responses of Lolium perenne L. exposed to cadmium (Cd2+) and mercury (Hg2+)
title_fullStr	Gene expression and morphological responses of Lolium perenne L. exposed to cadmium (Cd2+) and mercury (Hg2+)
title_full_unstemmed	Gene expression and morphological responses of Lolium perenne L. exposed to cadmium (Cd2+) and mercury (Hg2+)
title_sort	gene expression and morphological responses of lolium perenne l. exposed to cadmium (cd2+) and mercury (hg2+)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/098859ec5c2b4778adc33acfdd592b27
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Gene expression and morphological responses of Lolium perenne L. exposed to cadmium (Cd2+) and mercury (Hg2+)

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