Development and validation of a multiplex 19 X-chromosomal short tandem repeats typing system for forensic purposes

Abstract X-chromosome short tandem repeat (X-STR) markers are a powerful complementary system used for paternity and forensic casework. This study presents the development and validation of a new highly efficient multiplex-fluorescent-labeled 19 X-STR typing system, including DXS10079, DXS101, DXS10...

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Autores principales:	Juan Jia, Xu Liu, Qingwei Fan, Chen Fang, Mengchun Wang, Jiarong Zhang, Wanting Li, Linyu Shi, Xiaomeng Zhang, Chuguang Chen, Zailiang Yu, Chen Li, Keming Yun, Jiangwei Yan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0991af8a69384663819c69e5a535f2be
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spelling	oai:doaj.org-article:0991af8a69384663819c69e5a535f2be2021-12-02T15:23:06ZDevelopment and validation of a multiplex 19 X-chromosomal short tandem repeats typing system for forensic purposes10.1038/s41598-020-80414-x2045-2322https://doaj.org/article/0991af8a69384663819c69e5a535f2be2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80414-xhttps://doaj.org/toc/2045-2322Abstract X-chromosome short tandem repeat (X-STR) markers are a powerful complementary system used for paternity and forensic casework. This study presents the development and validation of a new highly efficient multiplex-fluorescent-labeled 19 X-STR typing system, including DXS10079, DXS101, DXS10135, DXS10162, DXS6795, DXS6800, DXS6803, DXS6807, DXS6809, DXS6810, DXS7133, DXS7423, DXS981, DXS9902, DXS9907, GATA165B12, GATA172D05, GATA31E08 and HPRTB along with sex-typing locus, amelogenin. The system was validated according to guidelines issued by the Scientific Working Group on DNA Analysis Methods. Allele frequency and forensic parameters were investigated from 1085 (494 males and 591 females) unrelated Beijing Han individuals, the combined power of discrimination by the 19 X-STR loci in females and males, as well as the combined mean exclusion chance in trios and duos, were 0.999999999999999995, 0.99999999995, 0.9999999995, and 0.9999996, respectively. The results demonstrate that this multiplex system is robust and reliable, and considered to be a powerful tool for forensic application.Juan JiaXu LiuQingwei FanChen FangMengchun WangJiarong ZhangWanting LiLinyu ShiXiaomeng ZhangChuguang ChenZailiang YuChen LiKeming YunJiangwei YanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juan Jia Xu Liu Qingwei Fan Chen Fang Mengchun Wang Jiarong Zhang Wanting Li Linyu Shi Xiaomeng Zhang Chuguang Chen Zailiang Yu Chen Li Keming Yun Jiangwei Yan Development and validation of a multiplex 19 X-chromosomal short tandem repeats typing system for forensic purposes
description	Abstract X-chromosome short tandem repeat (X-STR) markers are a powerful complementary system used for paternity and forensic casework. This study presents the development and validation of a new highly efficient multiplex-fluorescent-labeled 19 X-STR typing system, including DXS10079, DXS101, DXS10135, DXS10162, DXS6795, DXS6800, DXS6803, DXS6807, DXS6809, DXS6810, DXS7133, DXS7423, DXS981, DXS9902, DXS9907, GATA165B12, GATA172D05, GATA31E08 and HPRTB along with sex-typing locus, amelogenin. The system was validated according to guidelines issued by the Scientific Working Group on DNA Analysis Methods. Allele frequency and forensic parameters were investigated from 1085 (494 males and 591 females) unrelated Beijing Han individuals, the combined power of discrimination by the 19 X-STR loci in females and males, as well as the combined mean exclusion chance in trios and duos, were 0.999999999999999995, 0.99999999995, 0.9999999995, and 0.9999996, respectively. The results demonstrate that this multiplex system is robust and reliable, and considered to be a powerful tool for forensic application.
format	article
author	Juan Jia Xu Liu Qingwei Fan Chen Fang Mengchun Wang Jiarong Zhang Wanting Li Linyu Shi Xiaomeng Zhang Chuguang Chen Zailiang Yu Chen Li Keming Yun Jiangwei Yan
author_facet	Juan Jia Xu Liu Qingwei Fan Chen Fang Mengchun Wang Jiarong Zhang Wanting Li Linyu Shi Xiaomeng Zhang Chuguang Chen Zailiang Yu Chen Li Keming Yun Jiangwei Yan
author_sort	Juan Jia
title	Development and validation of a multiplex 19 X-chromosomal short tandem repeats typing system for forensic purposes
title_short	Development and validation of a multiplex 19 X-chromosomal short tandem repeats typing system for forensic purposes
title_full	Development and validation of a multiplex 19 X-chromosomal short tandem repeats typing system for forensic purposes
title_fullStr	Development and validation of a multiplex 19 X-chromosomal short tandem repeats typing system for forensic purposes
title_full_unstemmed	Development and validation of a multiplex 19 X-chromosomal short tandem repeats typing system for forensic purposes
title_sort	development and validation of a multiplex 19 x-chromosomal short tandem repeats typing system for forensic purposes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0991af8a69384663819c69e5a535f2be
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Development and validation of a multiplex 19 X-chromosomal short tandem repeats typing system for forensic purposes

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