Involvement of the Heat Shock Protein HtpG of Salmonella Typhimurium in Infection and Proliferation in Hosts
Salmonella Typhimurium is a common pathogen infecting the gastrointestinal tract of humans and animals, causing host gastroenteritis and typhoid fever. Heat shock protein (HtpG) as a molecular chaperone is involved in the various cellular processes of bacteria, especially under environmental stress....
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Frontiers Media S.A.
2021
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oai:doaj.org-article:09a1025f69c14b76b39971b05975b97a2021-11-16T06:40:56ZInvolvement of the Heat Shock Protein HtpG of Salmonella Typhimurium in Infection and Proliferation in Hosts2235-298810.3389/fcimb.2021.758898https://doaj.org/article/09a1025f69c14b76b39971b05975b97a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.758898/fullhttps://doaj.org/toc/2235-2988Salmonella Typhimurium is a common pathogen infecting the gastrointestinal tract of humans and animals, causing host gastroenteritis and typhoid fever. Heat shock protein (HtpG) as a molecular chaperone is involved in the various cellular processes of bacteria, especially under environmental stress. However, the potential association of HtpG with S. Typhimurium infection remains unknown. In this study, we clarified that HtpG could also play a role as an effector in S. Typhimurium infection. RNA-seq indicated that the flagellar assembly pathway, infection pathway, and chemotaxis pathway genes of S. Typhimurium were downregulated after the mutation of HtpG, which resulted in compromises of S. Typhimurium motility, biofilm formation, adhesion, invasion, and inflammation-inducing ability. In addition, HtpG recombinant protein was capable of promoting the proliferation of S. Typhimurium in host cells and the resultant inflammation. Collectively, our results illustrated an important role of HtpG in S. Typhimurium infection.Tao DongTao DongWeiwei WangWeiwei WangMinhao XiaMinhao XiaShujie LiangShujie LiangGuangzhong HuGuangzhong HuHui YeHui YeQingyun CaoQingyun CaoZemin DongZemin DongChangming ZhangChangming ZhangDingyuan FengDingyuan FengJianjun ZuoJianjun ZuoFrontiers Media S.A.articleSalmonella TyphimuriumHtpGRNA-seqinfectionimmunityMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021) |
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Salmonella Typhimurium HtpG RNA-seq infection immunity Microbiology QR1-502 |
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Salmonella Typhimurium HtpG RNA-seq infection immunity Microbiology QR1-502 Tao Dong Tao Dong Weiwei Wang Weiwei Wang Minhao Xia Minhao Xia Shujie Liang Shujie Liang Guangzhong Hu Guangzhong Hu Hui Ye Hui Ye Qingyun Cao Qingyun Cao Zemin Dong Zemin Dong Changming Zhang Changming Zhang Dingyuan Feng Dingyuan Feng Jianjun Zuo Jianjun Zuo Involvement of the Heat Shock Protein HtpG of Salmonella Typhimurium in Infection and Proliferation in Hosts |
| description |
Salmonella Typhimurium is a common pathogen infecting the gastrointestinal tract of humans and animals, causing host gastroenteritis and typhoid fever. Heat shock protein (HtpG) as a molecular chaperone is involved in the various cellular processes of bacteria, especially under environmental stress. However, the potential association of HtpG with S. Typhimurium infection remains unknown. In this study, we clarified that HtpG could also play a role as an effector in S. Typhimurium infection. RNA-seq indicated that the flagellar assembly pathway, infection pathway, and chemotaxis pathway genes of S. Typhimurium were downregulated after the mutation of HtpG, which resulted in compromises of S. Typhimurium motility, biofilm formation, adhesion, invasion, and inflammation-inducing ability. In addition, HtpG recombinant protein was capable of promoting the proliferation of S. Typhimurium in host cells and the resultant inflammation. Collectively, our results illustrated an important role of HtpG in S. Typhimurium infection. |
| format |
article |
| author |
Tao Dong Tao Dong Weiwei Wang Weiwei Wang Minhao Xia Minhao Xia Shujie Liang Shujie Liang Guangzhong Hu Guangzhong Hu Hui Ye Hui Ye Qingyun Cao Qingyun Cao Zemin Dong Zemin Dong Changming Zhang Changming Zhang Dingyuan Feng Dingyuan Feng Jianjun Zuo Jianjun Zuo |
| author_facet |
Tao Dong Tao Dong Weiwei Wang Weiwei Wang Minhao Xia Minhao Xia Shujie Liang Shujie Liang Guangzhong Hu Guangzhong Hu Hui Ye Hui Ye Qingyun Cao Qingyun Cao Zemin Dong Zemin Dong Changming Zhang Changming Zhang Dingyuan Feng Dingyuan Feng Jianjun Zuo Jianjun Zuo |
| author_sort |
Tao Dong |
| title |
Involvement of the Heat Shock Protein HtpG of Salmonella Typhimurium in Infection and Proliferation in Hosts |
| title_short |
Involvement of the Heat Shock Protein HtpG of Salmonella Typhimurium in Infection and Proliferation in Hosts |
| title_full |
Involvement of the Heat Shock Protein HtpG of Salmonella Typhimurium in Infection and Proliferation in Hosts |
| title_fullStr |
Involvement of the Heat Shock Protein HtpG of Salmonella Typhimurium in Infection and Proliferation in Hosts |
| title_full_unstemmed |
Involvement of the Heat Shock Protein HtpG of Salmonella Typhimurium in Infection and Proliferation in Hosts |
| title_sort |
involvement of the heat shock protein htpg of salmonella typhimurium in infection and proliferation in hosts |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/09a1025f69c14b76b39971b05975b97a |
| work_keys_str_mv |
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