Integrated de novo Analysis of Transcriptional and Metabolic Variations in Salt-Treated Solenostemma argel Desert Plants

Solenostemma argel (Delile) Hayne is a desert plant that survives harsh environmental conditions with several vital medicinal properties. Salt stress is a major constraint limiting agricultural production around the globe. However, response mechanisms behind the adaptation of S. argel plants to salt...

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Autores principales:	Hasan Ahmad, Mohamed Maher, Eslam M. Abdel-Salam, Yufei Li, Chenkun Yang, Nagwa ElSafty, Mohamed Ewas, Elsayed Nishawy, Jie Luo
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	transcriptome metabolome phenylpropanoid salt stress Solenostemma argel Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/09a137d732c74bffb859a4a203a3edb7
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spelling	oai:doaj.org-article:09a137d732c74bffb859a4a203a3edb72021-11-19T05:53:34ZIntegrated de novo Analysis of Transcriptional and Metabolic Variations in Salt-Treated Solenostemma argel Desert Plants1664-462X10.3389/fpls.2021.744699https://doaj.org/article/09a137d732c74bffb859a4a203a3edb72021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.744699/fullhttps://doaj.org/toc/1664-462XSolenostemma argel (Delile) Hayne is a desert plant that survives harsh environmental conditions with several vital medicinal properties. Salt stress is a major constraint limiting agricultural production around the globe. However, response mechanisms behind the adaptation of S. argel plants to salt stress are still poorly understood. In the current study, we applied an omics approach to explore how this plant adapts to salt stress by integrating transcriptomic and metabolomic changes in the roots and leaves of S. argel plants under salt stress. De novo assembly of transcriptome produced 57,796 unigenes represented by 165,147 transcripts/isoforms. A total of 730 differentially expressed genes (DEGs) were identified in the roots (396 and 334 were up- and down-regulated, respectively). In the leaves, 927 DEGs were identified (601 and 326 were up- and down-regulated, respectively). Gene ontology and Kyoto Encyclopedia of Genes And Genomes pathway enrichment analyses revealed that several defense-related biological processes, such as response to osmotic and oxidative stress, hormonal signal transduction, mitogen-activated protein kinase signaling, and phenylpropanoid biosynthesis pathways are the potential mechanisms involved in the tolerance of S. argel plants to salt stress. Furthermore, liquid chromatography-tandem mass spectrometry was used to detect the metabolic variations of the leaves and roots of S. argel under control and salt stress. 45 and 56 critical metabolites showed changes in their levels in the stressed roots and leaves, respectively; there were 20 metabolites in common between the roots and leaves. Differentially accumulated metabolites included amino acids, polyamines, hydroxycinnamic acids, monolignols, flavonoids, and saccharides that improve antioxidant ability and osmotic adjustment of S. argel plants under salt stress. The results present insights into potential salt response mechanisms in S. argel desert plants and increase the knowledge in order to generate more tolerant crops to salt stress.Hasan AhmadHasan AhmadMohamed MaherMohamed MaherEslam M. Abdel-SalamYufei LiChenkun YangNagwa ElSaftyMohamed EwasMohamed EwasElsayed NishawyElsayed NishawyJie LuoJie LuoFrontiers Media S.A.articletranscriptomemetabolomephenylpropanoidsalt stressSolenostemma argelPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	transcriptome metabolome phenylpropanoid salt stress Solenostemma argel Plant culture SB1-1110
spellingShingle	transcriptome metabolome phenylpropanoid salt stress Solenostemma argel Plant culture SB1-1110 Hasan Ahmad Hasan Ahmad Mohamed Maher Mohamed Maher Eslam M. Abdel-Salam Yufei Li Chenkun Yang Nagwa ElSafty Mohamed Ewas Mohamed Ewas Elsayed Nishawy Elsayed Nishawy Jie Luo Jie Luo Integrated de novo Analysis of Transcriptional and Metabolic Variations in Salt-Treated Solenostemma argel Desert Plants
description	Solenostemma argel (Delile) Hayne is a desert plant that survives harsh environmental conditions with several vital medicinal properties. Salt stress is a major constraint limiting agricultural production around the globe. However, response mechanisms behind the adaptation of S. argel plants to salt stress are still poorly understood. In the current study, we applied an omics approach to explore how this plant adapts to salt stress by integrating transcriptomic and metabolomic changes in the roots and leaves of S. argel plants under salt stress. De novo assembly of transcriptome produced 57,796 unigenes represented by 165,147 transcripts/isoforms. A total of 730 differentially expressed genes (DEGs) were identified in the roots (396 and 334 were up- and down-regulated, respectively). In the leaves, 927 DEGs were identified (601 and 326 were up- and down-regulated, respectively). Gene ontology and Kyoto Encyclopedia of Genes And Genomes pathway enrichment analyses revealed that several defense-related biological processes, such as response to osmotic and oxidative stress, hormonal signal transduction, mitogen-activated protein kinase signaling, and phenylpropanoid biosynthesis pathways are the potential mechanisms involved in the tolerance of S. argel plants to salt stress. Furthermore, liquid chromatography-tandem mass spectrometry was used to detect the metabolic variations of the leaves and roots of S. argel under control and salt stress. 45 and 56 critical metabolites showed changes in their levels in the stressed roots and leaves, respectively; there were 20 metabolites in common between the roots and leaves. Differentially accumulated metabolites included amino acids, polyamines, hydroxycinnamic acids, monolignols, flavonoids, and saccharides that improve antioxidant ability and osmotic adjustment of S. argel plants under salt stress. The results present insights into potential salt response mechanisms in S. argel desert plants and increase the knowledge in order to generate more tolerant crops to salt stress.
format	article
author	Hasan Ahmad Hasan Ahmad Mohamed Maher Mohamed Maher Eslam M. Abdel-Salam Yufei Li Chenkun Yang Nagwa ElSafty Mohamed Ewas Mohamed Ewas Elsayed Nishawy Elsayed Nishawy Jie Luo Jie Luo
author_facet	Hasan Ahmad Hasan Ahmad Mohamed Maher Mohamed Maher Eslam M. Abdel-Salam Yufei Li Chenkun Yang Nagwa ElSafty Mohamed Ewas Mohamed Ewas Elsayed Nishawy Elsayed Nishawy Jie Luo Jie Luo
author_sort	Hasan Ahmad
title	Integrated de novo Analysis of Transcriptional and Metabolic Variations in Salt-Treated Solenostemma argel Desert Plants
title_short	Integrated de novo Analysis of Transcriptional and Metabolic Variations in Salt-Treated Solenostemma argel Desert Plants
title_full	Integrated de novo Analysis of Transcriptional and Metabolic Variations in Salt-Treated Solenostemma argel Desert Plants
title_fullStr	Integrated de novo Analysis of Transcriptional and Metabolic Variations in Salt-Treated Solenostemma argel Desert Plants
title_full_unstemmed	Integrated de novo Analysis of Transcriptional and Metabolic Variations in Salt-Treated Solenostemma argel Desert Plants
title_sort	integrated de novo analysis of transcriptional and metabolic variations in salt-treated solenostemma argel desert plants
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/09a137d732c74bffb859a4a203a3edb7
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Integrated de novo Analysis of Transcriptional and Metabolic Variations in Salt-Treated Solenostemma argel Desert Plants

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