A multielectrode array microchannel platform reveals both transient and slow changes in axonal conduction velocity

Abstract Due to their small dimensions, electrophysiology on thin and intricate axonal branches in support of understanding their role in normal and diseased brain function poses experimental challenges. To reduce experimental complexity, we coupled microelectrode arrays (MEAs) to bi-level microchan...

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Autores principales:	Rouhollah Habibey, Shahrzad Latifi, Hossein Mousavi, Mattia Pesce, Elmira Arab-Tehrany, Axel Blau
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/09a93051169d47b2a2852f651f09826f
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spelling	oai:doaj.org-article:09a93051169d47b2a2852f651f09826f2021-12-02T16:07:05ZA multielectrode array microchannel platform reveals both transient and slow changes in axonal conduction velocity10.1038/s41598-017-09033-32045-2322https://doaj.org/article/09a93051169d47b2a2852f651f09826f2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09033-3https://doaj.org/toc/2045-2322Abstract Due to their small dimensions, electrophysiology on thin and intricate axonal branches in support of understanding their role in normal and diseased brain function poses experimental challenges. To reduce experimental complexity, we coupled microelectrode arrays (MEAs) to bi-level microchannel devices for the long-term in vitro tracking of axonal morphology and activity with high spatiotemporal resolution. Our model allowed the long-term multisite recording from pure axonal branches in a microscopy-compatible environment. Compartmentalizing the network structure into interconnected subpopulations simplified access to the locations of interest. Electrophysiological data over 95 days in vitro (DIV) showed an age-dependent increase of axonal conduction velocity, which was positively correlated with, but independent of evolving burst activity over time. Conduction velocity remained constant at chemically increased network activity levels. In contrast, low frequency (1 Hz, 180 repetitions) electrical stimulation of axons or network subpopulations evoked amplitude-dependent direct (5–35 ms peri-stimulus) and polysynaptic (35–1,000 ms peri-stimulus) activity with temporarily (<35 ms) elevated propagation velocities along the perisomatic branches. Furthermore, effective stimulation amplitudes were found to be significantly lower (>250 mV) in microchannels when compared with those reported for unconfined cultures (>800 mV). The experimental paradigm may lead to new insights into stimulation-induced axonal plasticity.Rouhollah HabibeyShahrzad LatifiHossein MousaviMattia PesceElmira Arab-TehranyAxel BlauNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rouhollah Habibey Shahrzad Latifi Hossein Mousavi Mattia Pesce Elmira Arab-Tehrany Axel Blau A multielectrode array microchannel platform reveals both transient and slow changes in axonal conduction velocity
description	Abstract Due to their small dimensions, electrophysiology on thin and intricate axonal branches in support of understanding their role in normal and diseased brain function poses experimental challenges. To reduce experimental complexity, we coupled microelectrode arrays (MEAs) to bi-level microchannel devices for the long-term in vitro tracking of axonal morphology and activity with high spatiotemporal resolution. Our model allowed the long-term multisite recording from pure axonal branches in a microscopy-compatible environment. Compartmentalizing the network structure into interconnected subpopulations simplified access to the locations of interest. Electrophysiological data over 95 days in vitro (DIV) showed an age-dependent increase of axonal conduction velocity, which was positively correlated with, but independent of evolving burst activity over time. Conduction velocity remained constant at chemically increased network activity levels. In contrast, low frequency (1 Hz, 180 repetitions) electrical stimulation of axons or network subpopulations evoked amplitude-dependent direct (5–35 ms peri-stimulus) and polysynaptic (35–1,000 ms peri-stimulus) activity with temporarily (<35 ms) elevated propagation velocities along the perisomatic branches. Furthermore, effective stimulation amplitudes were found to be significantly lower (>250 mV) in microchannels when compared with those reported for unconfined cultures (>800 mV). The experimental paradigm may lead to new insights into stimulation-induced axonal plasticity.
format	article
author	Rouhollah Habibey Shahrzad Latifi Hossein Mousavi Mattia Pesce Elmira Arab-Tehrany Axel Blau
author_facet	Rouhollah Habibey Shahrzad Latifi Hossein Mousavi Mattia Pesce Elmira Arab-Tehrany Axel Blau
author_sort	Rouhollah Habibey
title	A multielectrode array microchannel platform reveals both transient and slow changes in axonal conduction velocity
title_short	A multielectrode array microchannel platform reveals both transient and slow changes in axonal conduction velocity
title_full	A multielectrode array microchannel platform reveals both transient and slow changes in axonal conduction velocity
title_fullStr	A multielectrode array microchannel platform reveals both transient and slow changes in axonal conduction velocity
title_full_unstemmed	A multielectrode array microchannel platform reveals both transient and slow changes in axonal conduction velocity
title_sort	multielectrode array microchannel platform reveals both transient and slow changes in axonal conduction velocity
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/09a93051169d47b2a2852f651f09826f
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A multielectrode array microchannel platform reveals both transient and slow changes in axonal conduction velocity

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