Quorum sensing systems and related virulence factors in Pseudomonas aeruginosa isolated from chicken meat and ground beef

Abstract The objective of this study was to evaluate 50 [chicken meat (n = 45) and ground beef (n = 5)] Pseudomonas aeruginosa isolates to determine the expression of the lasI and rhl QS systems, related virulence factors, and the presence of N-3-oxo-dodecanoyl homoserine lactone (AHL: 3-O-C12-HSL)....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gökhan İnat, Belgin Sırıken, Ceren Başkan, İrfan Erol, Tuba Yıldırım, Alper Çiftci
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/09a9df3048784feb830e7ebd8bb37c65
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:09a9df3048784feb830e7ebd8bb37c65
record_format	dspace
spelling	oai:doaj.org-article:09a9df3048784feb830e7ebd8bb37c652021-12-02T17:06:32ZQuorum sensing systems and related virulence factors in Pseudomonas aeruginosa isolated from chicken meat and ground beef10.1038/s41598-021-94906-x2045-2322https://doaj.org/article/09a9df3048784feb830e7ebd8bb37c652021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94906-xhttps://doaj.org/toc/2045-2322Abstract The objective of this study was to evaluate 50 [chicken meat (n = 45) and ground beef (n = 5)] Pseudomonas aeruginosa isolates to determine the expression of the lasI and rhl QS systems, related virulence factors, and the presence of N-3-oxo-dodecanoyl homoserine lactone (AHL: 3-O-C12-HSL). For the isolation and identification of P. aeruginosa, conventional culture and oprL gene-based molecular techniques were used. In relation to QS systems, eight genes consisting of four intact and four internal (lasI/R, rhlI/R) genes were analyzed with PCR assay. The two QS systems genes in P. aeruginosa isolates from ground beef (80.00%) and chicken meat (76.00%) were present at quite high levels. The 3-O-C12-HSL was detected in 14.00% of the isolates. Both biofilm formation and motility were detected in 98.00% of the isolates. Protease activity was determined in 54.00% of the isolates. Pyocyanin production was detected in 48.00% of the isolates. The las system scores strongly and positively correlated with the rhl system (p ˂ .01). PYA moderately and positively correlated with protease (p ˂ .05). In addition, there was statistically significance between lasI and protease activity (p < .10), and rhlI and twitching motility (p < .10). In conclusion, the high number of isolates having QS systems and related virulence factors are critical for public health. Pyocyanin, protease, and biofilm formation can cause spoilage and play essential role in food spoilage and food safety.Gökhan İnatBelgin SırıkenCeren Başkanİrfan ErolTuba YıldırımAlper ÇiftciNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gökhan İnat Belgin Sırıken Ceren Başkan İrfan Erol Tuba Yıldırım Alper Çiftci Quorum sensing systems and related virulence factors in Pseudomonas aeruginosa isolated from chicken meat and ground beef
description	Abstract The objective of this study was to evaluate 50 [chicken meat (n = 45) and ground beef (n = 5)] Pseudomonas aeruginosa isolates to determine the expression of the lasI and rhl QS systems, related virulence factors, and the presence of N-3-oxo-dodecanoyl homoserine lactone (AHL: 3-O-C12-HSL). For the isolation and identification of P. aeruginosa, conventional culture and oprL gene-based molecular techniques were used. In relation to QS systems, eight genes consisting of four intact and four internal (lasI/R, rhlI/R) genes were analyzed with PCR assay. The two QS systems genes in P. aeruginosa isolates from ground beef (80.00%) and chicken meat (76.00%) were present at quite high levels. The 3-O-C12-HSL was detected in 14.00% of the isolates. Both biofilm formation and motility were detected in 98.00% of the isolates. Protease activity was determined in 54.00% of the isolates. Pyocyanin production was detected in 48.00% of the isolates. The las system scores strongly and positively correlated with the rhl system (p ˂ .01). PYA moderately and positively correlated with protease (p ˂ .05). In addition, there was statistically significance between lasI and protease activity (p < .10), and rhlI and twitching motility (p < .10). In conclusion, the high number of isolates having QS systems and related virulence factors are critical for public health. Pyocyanin, protease, and biofilm formation can cause spoilage and play essential role in food spoilage and food safety.
format	article
author	Gökhan İnat Belgin Sırıken Ceren Başkan İrfan Erol Tuba Yıldırım Alper Çiftci
author_facet	Gökhan İnat Belgin Sırıken Ceren Başkan İrfan Erol Tuba Yıldırım Alper Çiftci
author_sort	Gökhan İnat
title	Quorum sensing systems and related virulence factors in Pseudomonas aeruginosa isolated from chicken meat and ground beef
title_short	Quorum sensing systems and related virulence factors in Pseudomonas aeruginosa isolated from chicken meat and ground beef
title_full	Quorum sensing systems and related virulence factors in Pseudomonas aeruginosa isolated from chicken meat and ground beef
title_fullStr	Quorum sensing systems and related virulence factors in Pseudomonas aeruginosa isolated from chicken meat and ground beef
title_full_unstemmed	Quorum sensing systems and related virulence factors in Pseudomonas aeruginosa isolated from chicken meat and ground beef
title_sort	quorum sensing systems and related virulence factors in pseudomonas aeruginosa isolated from chicken meat and ground beef
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/09a9df3048784feb830e7ebd8bb37c65
work_keys_str_mv	AT gokhaninat quorumsensingsystemsandrelatedvirulencefactorsinpseudomonasaeruginosaisolatedfromchickenmeatandgroundbeef AT belginsırıken quorumsensingsystemsandrelatedvirulencefactorsinpseudomonasaeruginosaisolatedfromchickenmeatandgroundbeef AT cerenbaskan quorumsensingsystemsandrelatedvirulencefactorsinpseudomonasaeruginosaisolatedfromchickenmeatandgroundbeef AT irfanerol quorumsensingsystemsandrelatedvirulencefactorsinpseudomonasaeruginosaisolatedfromchickenmeatandgroundbeef AT tubayıldırım quorumsensingsystemsandrelatedvirulencefactorsinpseudomonasaeruginosaisolatedfromchickenmeatandgroundbeef AT alperciftci quorumsensingsystemsandrelatedvirulencefactorsinpseudomonasaeruginosaisolatedfromchickenmeatandgroundbeef
_version_	1718381537675706368

Quorum sensing systems and related virulence factors in Pseudomonas aeruginosa isolated from chicken meat and ground beef

Ejemplares similares