Amperometric urea biosensors based on sulfonated graphene/polyaniline nanocomposite

Gautam Das, Hyon Hee Yoon Department of Chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi-do, South Korea Abstract: An electrochemical biosensor based on sulfonated graphene/polyaniline nanocomposite was developed for urea analysis. Oxidative polymerization of aniline in t...

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Autores principales:	Das G, Yoon HH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/09aa4999b4094a739fc4663631e4a473
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spelling	oai:doaj.org-article:09aa4999b4094a739fc4663631e4a4732021-12-02T02:29:09ZAmperometric urea biosensors based on sulfonated graphene/polyaniline nanocomposite1178-2013https://doaj.org/article/09aa4999b4094a739fc4663631e4a4732015-08-01T00:00:00Zhttp://www.dovepress.com/amperometric-urea-biosensors-based-on-sulfonated-graphenepolyaniline-n-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Gautam Das, Hyon Hee Yoon Department of Chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi-do, South Korea Abstract: An electrochemical biosensor based on sulfonated graphene/polyaniline nanocomposite was developed for urea analysis. Oxidative polymerization of aniline in the presence of sulfonated graphene oxide was carried out by electrochemical methods in an aqueous environment. The structural properties of the nanocomposite were characterized by Fourier-transform infrared, Raman spectroscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy techniques. The urease enzyme-immobilized sulfonated graphene/polyaniline nanocomposite film showed impressive performance in the electroanalytical detection of urea with a detection limit of 0.050 mM and a sensitivity of 0.85 µA·cm-2·mM-1. The biosensor achieved a broad linear range of detection (0.12–12.3 mM) with a notable response time of approximately 5 seconds. Moreover, the fabricated biosensor retained 81% of its initial activity (based on sensitivity) after 15 days of storage at 4°C. The ease of fabrication coupled with the low cost and good electrochemical performance of this system holds potential for the development of solid-state biosensors for urea detection. Keywords: electrochemical deposition, sulfonated graphene oxide, ureaseDas GYoon HHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss Special Issue on diverse applications in Nano-Theranostics, Pp 55-66 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Das G Yoon HH Amperometric urea biosensors based on sulfonated graphene/polyaniline nanocomposite
description	Gautam Das, Hyon Hee Yoon Department of Chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi-do, South Korea Abstract: An electrochemical biosensor based on sulfonated graphene/polyaniline nanocomposite was developed for urea analysis. Oxidative polymerization of aniline in the presence of sulfonated graphene oxide was carried out by electrochemical methods in an aqueous environment. The structural properties of the nanocomposite were characterized by Fourier-transform infrared, Raman spectroscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy techniques. The urease enzyme-immobilized sulfonated graphene/polyaniline nanocomposite film showed impressive performance in the electroanalytical detection of urea with a detection limit of 0.050 mM and a sensitivity of 0.85 µA·cm-2·mM-1. The biosensor achieved a broad linear range of detection (0.12–12.3 mM) with a notable response time of approximately 5 seconds. Moreover, the fabricated biosensor retained 81% of its initial activity (based on sensitivity) after 15 days of storage at 4°C. The ease of fabrication coupled with the low cost and good electrochemical performance of this system holds potential for the development of solid-state biosensors for urea detection. Keywords: electrochemical deposition, sulfonated graphene oxide, urease
format	article
author	Das G Yoon HH
author_facet	Das G Yoon HH
author_sort	Das G
title	Amperometric urea biosensors based on sulfonated graphene/polyaniline nanocomposite
title_short	Amperometric urea biosensors based on sulfonated graphene/polyaniline nanocomposite
title_full	Amperometric urea biosensors based on sulfonated graphene/polyaniline nanocomposite
title_fullStr	Amperometric urea biosensors based on sulfonated graphene/polyaniline nanocomposite
title_full_unstemmed	Amperometric urea biosensors based on sulfonated graphene/polyaniline nanocomposite
title_sort	amperometric urea biosensors based on sulfonated graphene/polyaniline nanocomposite
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/09aa4999b4094a739fc4663631e4a473
work_keys_str_mv	AT dasg amperometricureabiosensorsbasedonsulfonatedgraphenepolyanilinenanocomposite AT yoonhh amperometricureabiosensorsbasedonsulfonatedgraphenepolyanilinenanocomposite
_version_	1718402448890003456

Amperometric urea biosensors based on sulfonated graphene/polyaniline nanocomposite

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