Bacterial challenge-associated metabolic phenotypes in Hermetia illucens defining nutritional and functional benefits
Abstract Black soldier fly (BSF, Hermetia illucens) is popular for its applications in animal feed, waste management and antimicrobial peptide source. The major advantages of BSF larva include their robust immune system and high nutritional content that can be further developed into more potential a...
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2021
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oai:doaj.org-article:09b57170654648ff8010666bc57687b22021-12-05T12:14:23ZBacterial challenge-associated metabolic phenotypes in Hermetia illucens defining nutritional and functional benefits10.1038/s41598-021-02752-82045-2322https://doaj.org/article/09b57170654648ff8010666bc57687b22021-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02752-8https://doaj.org/toc/2045-2322Abstract Black soldier fly (BSF, Hermetia illucens) is popular for its applications in animal feed, waste management and antimicrobial peptide source. The major advantages of BSF larva include their robust immune system and high nutritional content that can be further developed into more potential agricultural and medical applications. Several strategies are now being developed to exploit their fullest capabilities and one of these is the immunity modulation using bacterial challenges. The mechanism underlying metabolic responses of BSF to different bacteria has, however, remained unclear. In the current study, entometabolomics was employed to investigate the metabolic phenoconversion in response to either Escherichia coli, Staphylococcus aureus, or combined challenges in BSF larva. We have, thus far, characterised 37 metabolites in BSF larva challenged with different bacteria with the major biochemical groups consisting of amino acids, organic acids, and sugars. The distinct defense mechanism-specific metabolic phenotypes were clearly observed. The combined challenge contributed to the most significant metabolic phenoconversion in BSF larva with the dominant metabolic phenotypes induced by S. aureus. Our study suggested that the accumulation of energy-related metabolites provided by amino acid catabolism is the principal metabolic pathway regulating the defense mechanism. Therefore, combined challenge is strongly recommended for raising BSF immunity as it remarkably triggered amino acid metabolisms including arginine and proline metabolism and alanine, aspartate and glutamate metabolism along with purine metabolism and pyruvate metabolism that potentially result in the production of various nutritional and functional metabolites.Phuc N. HoPoramate KlanritYupa HanboonsongUmaporn YordpratumManida SuksawatThanaporn KulthawatsiriAnyarin JirahiranpatSuthicha DeewaiPanya MackawanRasana W. SermswanNisana NamwatWatcharin LoilomeTueanjit KhampitakArporn WangwiwatsinJutarop PhetcharaburaninNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
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Medicine R Science Q Phuc N. Ho Poramate Klanrit Yupa Hanboonsong Umaporn Yordpratum Manida Suksawat Thanaporn Kulthawatsiri Anyarin Jirahiranpat Suthicha Deewai Panya Mackawan Rasana W. Sermswan Nisana Namwat Watcharin Loilome Tueanjit Khampitak Arporn Wangwiwatsin Jutarop Phetcharaburanin Bacterial challenge-associated metabolic phenotypes in Hermetia illucens defining nutritional and functional benefits |
| description |
Abstract Black soldier fly (BSF, Hermetia illucens) is popular for its applications in animal feed, waste management and antimicrobial peptide source. The major advantages of BSF larva include their robust immune system and high nutritional content that can be further developed into more potential agricultural and medical applications. Several strategies are now being developed to exploit their fullest capabilities and one of these is the immunity modulation using bacterial challenges. The mechanism underlying metabolic responses of BSF to different bacteria has, however, remained unclear. In the current study, entometabolomics was employed to investigate the metabolic phenoconversion in response to either Escherichia coli, Staphylococcus aureus, or combined challenges in BSF larva. We have, thus far, characterised 37 metabolites in BSF larva challenged with different bacteria with the major biochemical groups consisting of amino acids, organic acids, and sugars. The distinct defense mechanism-specific metabolic phenotypes were clearly observed. The combined challenge contributed to the most significant metabolic phenoconversion in BSF larva with the dominant metabolic phenotypes induced by S. aureus. Our study suggested that the accumulation of energy-related metabolites provided by amino acid catabolism is the principal metabolic pathway regulating the defense mechanism. Therefore, combined challenge is strongly recommended for raising BSF immunity as it remarkably triggered amino acid metabolisms including arginine and proline metabolism and alanine, aspartate and glutamate metabolism along with purine metabolism and pyruvate metabolism that potentially result in the production of various nutritional and functional metabolites. |
| format |
article |
| author |
Phuc N. Ho Poramate Klanrit Yupa Hanboonsong Umaporn Yordpratum Manida Suksawat Thanaporn Kulthawatsiri Anyarin Jirahiranpat Suthicha Deewai Panya Mackawan Rasana W. Sermswan Nisana Namwat Watcharin Loilome Tueanjit Khampitak Arporn Wangwiwatsin Jutarop Phetcharaburanin |
| author_facet |
Phuc N. Ho Poramate Klanrit Yupa Hanboonsong Umaporn Yordpratum Manida Suksawat Thanaporn Kulthawatsiri Anyarin Jirahiranpat Suthicha Deewai Panya Mackawan Rasana W. Sermswan Nisana Namwat Watcharin Loilome Tueanjit Khampitak Arporn Wangwiwatsin Jutarop Phetcharaburanin |
| author_sort |
Phuc N. Ho |
| title |
Bacterial challenge-associated metabolic phenotypes in Hermetia illucens defining nutritional and functional benefits |
| title_short |
Bacterial challenge-associated metabolic phenotypes in Hermetia illucens defining nutritional and functional benefits |
| title_full |
Bacterial challenge-associated metabolic phenotypes in Hermetia illucens defining nutritional and functional benefits |
| title_fullStr |
Bacterial challenge-associated metabolic phenotypes in Hermetia illucens defining nutritional and functional benefits |
| title_full_unstemmed |
Bacterial challenge-associated metabolic phenotypes in Hermetia illucens defining nutritional and functional benefits |
| title_sort |
bacterial challenge-associated metabolic phenotypes in hermetia illucens defining nutritional and functional benefits |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/09b57170654648ff8010666bc57687b2 |
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