Multi-omic approach provides insights into osmoregulation and osmoconformation of the crab Scylla paramamosain

Abstract Osmoregulation and osmoconformation are two mechanisms through which aquatic animals adapt to salinity fluctuations. The euryhaline crab Scylla paramamosain, being both an osmoconformer and osmoregulator, is an excellent model organism to investigate salinity adaptation mechanisms in brachy...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jiaojiao Niu, Xue Lei Hu, Jack C. H. Ip, Ka Yan Ma, Yuanyuan Tang, Yaqin Wang, Jing Qin, Jian-Wen Qiu, Ting Fung Chan, Ka Hou Chu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/09c001c20cb94c4a985bfc447a622c78
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:09c001c20cb94c4a985bfc447a622c78
record_format	dspace
spelling	oai:doaj.org-article:09c001c20cb94c4a985bfc447a622c782021-12-02T11:43:43ZMulti-omic approach provides insights into osmoregulation and osmoconformation of the crab Scylla paramamosain10.1038/s41598-020-78351-w2045-2322https://doaj.org/article/09c001c20cb94c4a985bfc447a622c782020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78351-whttps://doaj.org/toc/2045-2322Abstract Osmoregulation and osmoconformation are two mechanisms through which aquatic animals adapt to salinity fluctuations. The euryhaline crab Scylla paramamosain, being both an osmoconformer and osmoregulator, is an excellent model organism to investigate salinity adaptation mechanisms in brachyurans. In the present study, we used transcriptomic and proteomic approaches to investigate the response of S. paramamosain to salinity stress. Crabs were transferred from a salinity of 25 ppt to salinities of 5 ppt or 33 ppt for 6 h and 10 days. Data from both approaches revealed that exposure to 5 ppt resulted in upregulation of ion transport and energy metabolism associated genes. Notably, acclimation to low salinity was associated with early changes in gene expression for signal transduction and stress response. In contrast, exposure to 33 ppt resulted in upregulation of genes related to amino acid metabolism, and amino acid transport genes were upregulated only at the early stage of acclimation to this salinity. Our study reveals contrasting mechanisms underlying osmoregulation and osmoconformation within the salinity range of 5–33 ppt in the mud crab, and provides novel candidate genes for osmotic signal transduction, thereby providing insights on understanding the salinity adaptation mechanisms of brachyuran crabs.Jiaojiao NiuXue Lei HuJack C. H. IpKa Yan MaYuanyuan TangYaqin WangJing QinJian-Wen QiuTing Fung ChanKa Hou ChuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiaojiao Niu Xue Lei Hu Jack C. H. Ip Ka Yan Ma Yuanyuan Tang Yaqin Wang Jing Qin Jian-Wen Qiu Ting Fung Chan Ka Hou Chu Multi-omic approach provides insights into osmoregulation and osmoconformation of the crab Scylla paramamosain
description	Abstract Osmoregulation and osmoconformation are two mechanisms through which aquatic animals adapt to salinity fluctuations. The euryhaline crab Scylla paramamosain, being both an osmoconformer and osmoregulator, is an excellent model organism to investigate salinity adaptation mechanisms in brachyurans. In the present study, we used transcriptomic and proteomic approaches to investigate the response of S. paramamosain to salinity stress. Crabs were transferred from a salinity of 25 ppt to salinities of 5 ppt or 33 ppt for 6 h and 10 days. Data from both approaches revealed that exposure to 5 ppt resulted in upregulation of ion transport and energy metabolism associated genes. Notably, acclimation to low salinity was associated with early changes in gene expression for signal transduction and stress response. In contrast, exposure to 33 ppt resulted in upregulation of genes related to amino acid metabolism, and amino acid transport genes were upregulated only at the early stage of acclimation to this salinity. Our study reveals contrasting mechanisms underlying osmoregulation and osmoconformation within the salinity range of 5–33 ppt in the mud crab, and provides novel candidate genes for osmotic signal transduction, thereby providing insights on understanding the salinity adaptation mechanisms of brachyuran crabs.
format	article
author	Jiaojiao Niu Xue Lei Hu Jack C. H. Ip Ka Yan Ma Yuanyuan Tang Yaqin Wang Jing Qin Jian-Wen Qiu Ting Fung Chan Ka Hou Chu
author_facet	Jiaojiao Niu Xue Lei Hu Jack C. H. Ip Ka Yan Ma Yuanyuan Tang Yaqin Wang Jing Qin Jian-Wen Qiu Ting Fung Chan Ka Hou Chu
author_sort	Jiaojiao Niu
title	Multi-omic approach provides insights into osmoregulation and osmoconformation of the crab Scylla paramamosain
title_short	Multi-omic approach provides insights into osmoregulation and osmoconformation of the crab Scylla paramamosain
title_full	Multi-omic approach provides insights into osmoregulation and osmoconformation of the crab Scylla paramamosain
title_fullStr	Multi-omic approach provides insights into osmoregulation and osmoconformation of the crab Scylla paramamosain
title_full_unstemmed	Multi-omic approach provides insights into osmoregulation and osmoconformation of the crab Scylla paramamosain
title_sort	multi-omic approach provides insights into osmoregulation and osmoconformation of the crab scylla paramamosain
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/09c001c20cb94c4a985bfc447a622c78
work_keys_str_mv	AT jiaojiaoniu multiomicapproachprovidesinsightsintoosmoregulationandosmoconformationofthecrabscyllaparamamosain AT xueleihu multiomicapproachprovidesinsightsintoosmoregulationandosmoconformationofthecrabscyllaparamamosain AT jackchip multiomicapproachprovidesinsightsintoosmoregulationandosmoconformationofthecrabscyllaparamamosain AT kayanma multiomicapproachprovidesinsightsintoosmoregulationandosmoconformationofthecrabscyllaparamamosain AT yuanyuantang multiomicapproachprovidesinsightsintoosmoregulationandosmoconformationofthecrabscyllaparamamosain AT yaqinwang multiomicapproachprovidesinsightsintoosmoregulationandosmoconformationofthecrabscyllaparamamosain AT jingqin multiomicapproachprovidesinsightsintoosmoregulationandosmoconformationofthecrabscyllaparamamosain AT jianwenqiu multiomicapproachprovidesinsightsintoosmoregulationandosmoconformationofthecrabscyllaparamamosain AT tingfungchan multiomicapproachprovidesinsightsintoosmoregulationandosmoconformationofthecrabscyllaparamamosain AT kahouchu multiomicapproachprovidesinsightsintoosmoregulationandosmoconformationofthecrabscyllaparamamosain
_version_	1718395323347369984

Multi-omic approach provides insights into osmoregulation and osmoconformation of the crab Scylla paramamosain

Ejemplares similares