A brief exposure to cadmium impairs Leydig cell regeneration in the adult rat testis

Abstract Cadmium is an endocrine disruptor, impairing male reproduction. The objective of this study is to investigate whether cadmium affects rat Leydig cell regeneration and to dissect the underlying mechanism. Adult male Sprague-Dawley rats received a single intraperitoneal injection (i.p.) of 0,...

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Autores principales:	Xiaolong Wu, Xiaoling Guo, Huang Wang, Songyi Zhou, Lili Li, Xiaomin Chen, Guimin Wang, Jianpeng Liu, Hong-Shan Ge, Ren-Shan Ge
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/09c5513f30884b788630f02f28fa1b31
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spelling	oai:doaj.org-article:09c5513f30884b788630f02f28fa1b312021-12-02T15:06:27ZA brief exposure to cadmium impairs Leydig cell regeneration in the adult rat testis10.1038/s41598-017-06870-02045-2322https://doaj.org/article/09c5513f30884b788630f02f28fa1b312017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06870-0https://doaj.org/toc/2045-2322Abstract Cadmium is an endocrine disruptor, impairing male reproduction. The objective of this study is to investigate whether cadmium affects rat Leydig cell regeneration and to dissect the underlying mechanism. Adult male Sprague-Dawley rats received a single intraperitoneal injection (i.p.) of 0, 0.5 or 1.0 mg/kg of cadmium chloride, followed by ethane dimethane sulfonate (EDS) treatment to eliminate adult Leydig cells 20 days later. Compared to control (0 dose), cadmium treatment reduced serum testosterone levels by days 21, 35, and 56 after EDS treatment. Serum luteinizing hormone (LH) levels were also affected by day 56, the only time point examined. There were fewer regenerated Leydig cells in the cadmium-treated testis on days 35 and 56 after EDS treatment. Further studies demonstrated that the mRNA or protein levels of Leydig (Lhcgr, Scarb1, Star, Cyp11a1, Hsd3b1, Cyp17a1, Hsd17b3, and Hsd11b1), non-Leydig (Fshr and Dhh), and gonadotroph (Lhb) cells were also significantly lower in cadmium-treated animals. Since LH and desert hedgehog (DHH) are critical factors for Leydig cell differentiation, our result demonstrated that the lower doses of cadmium exposure, even briefly, may permanently damage Leydig cell regeneration.Xiaolong WuXiaoling GuoHuang WangSongyi ZhouLili LiXiaomin ChenGuimin WangJianpeng LiuHong-Shan GeRen-Shan GeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaolong Wu Xiaoling Guo Huang Wang Songyi Zhou Lili Li Xiaomin Chen Guimin Wang Jianpeng Liu Hong-Shan Ge Ren-Shan Ge A brief exposure to cadmium impairs Leydig cell regeneration in the adult rat testis
description	Abstract Cadmium is an endocrine disruptor, impairing male reproduction. The objective of this study is to investigate whether cadmium affects rat Leydig cell regeneration and to dissect the underlying mechanism. Adult male Sprague-Dawley rats received a single intraperitoneal injection (i.p.) of 0, 0.5 or 1.0 mg/kg of cadmium chloride, followed by ethane dimethane sulfonate (EDS) treatment to eliminate adult Leydig cells 20 days later. Compared to control (0 dose), cadmium treatment reduced serum testosterone levels by days 21, 35, and 56 after EDS treatment. Serum luteinizing hormone (LH) levels were also affected by day 56, the only time point examined. There were fewer regenerated Leydig cells in the cadmium-treated testis on days 35 and 56 after EDS treatment. Further studies demonstrated that the mRNA or protein levels of Leydig (Lhcgr, Scarb1, Star, Cyp11a1, Hsd3b1, Cyp17a1, Hsd17b3, and Hsd11b1), non-Leydig (Fshr and Dhh), and gonadotroph (Lhb) cells were also significantly lower in cadmium-treated animals. Since LH and desert hedgehog (DHH) are critical factors for Leydig cell differentiation, our result demonstrated that the lower doses of cadmium exposure, even briefly, may permanently damage Leydig cell regeneration.
format	article
author	Xiaolong Wu Xiaoling Guo Huang Wang Songyi Zhou Lili Li Xiaomin Chen Guimin Wang Jianpeng Liu Hong-Shan Ge Ren-Shan Ge
author_facet	Xiaolong Wu Xiaoling Guo Huang Wang Songyi Zhou Lili Li Xiaomin Chen Guimin Wang Jianpeng Liu Hong-Shan Ge Ren-Shan Ge
author_sort	Xiaolong Wu
title	A brief exposure to cadmium impairs Leydig cell regeneration in the adult rat testis
title_short	A brief exposure to cadmium impairs Leydig cell regeneration in the adult rat testis
title_full	A brief exposure to cadmium impairs Leydig cell regeneration in the adult rat testis
title_fullStr	A brief exposure to cadmium impairs Leydig cell regeneration in the adult rat testis
title_full_unstemmed	A brief exposure to cadmium impairs Leydig cell regeneration in the adult rat testis
title_sort	brief exposure to cadmium impairs leydig cell regeneration in the adult rat testis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/09c5513f30884b788630f02f28fa1b31
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A brief exposure to cadmium impairs Leydig cell regeneration in the adult rat testis

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