Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway

Abstract Our aim was to investigate the effects of phycocyanin (PC) on bleomycin (BLM)-induced pulmonary fibrosis (PF). In this study, C57 BL/6 wild-type (WT) mice and toll-like receptor (TLR) 2 deficient mice were treated with PC for 28 days following BLM exposure. Serum and lung tissues were colle...

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Autores principales:	Chengcheng Li, Yan Yu, Wenjun Li, Bo Liu, Xudong Jiao, Xinyu Song, Changjun Lv, Song Qin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/09c77f351b4d4181b4675adfb5cd1473
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spelling	oai:doaj.org-article:09c77f351b4d4181b4675adfb5cd14732021-12-02T11:40:58ZPhycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway10.1038/s41598-017-06021-52045-2322https://doaj.org/article/09c77f351b4d4181b4675adfb5cd14732017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06021-5https://doaj.org/toc/2045-2322Abstract Our aim was to investigate the effects of phycocyanin (PC) on bleomycin (BLM)-induced pulmonary fibrosis (PF). In this study, C57 BL/6 wild-type (WT) mice and toll-like receptor (TLR) 2 deficient mice were treated with PC for 28 days following BLM exposure. Serum and lung tissues were collected on days 3, 7 and 28. Data shows PC significantly decreased the levels of hydroxyproline (HYP), vimentin, surfactant-associated protein C (SP-C), fibroblast specific protein-1 (S100A4) and α-smooth muscle actin (α-SMA) but dramatically increased E-cadherin and podoplanin (PDPN) expression on day 28. Moreover, PC greatly decreased the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and myeloperoxidase (MPO) at the earlier time. Reduced expression of key genes in the TLR2 pathway was also detected. Compared with WT mice, TLR2-deficient mice exhibited less injury, and the protective effect of PC was partly diminished in this background. These data indicate the anti-fibrotic effects of PC may be mediated by reducing W/D ratio, MPO, IL-6, TNF-α, protecting type I alveolar epithelial cells, inhibiting fibroblast proliferation, attenuating epithelial-mesenchymal transitions (EMT) and reducing oxidative stress. The TLR2-MyD88-NF-κB pathway plays an important role in PC-mediated reduction in pulmonary fibrosis.Chengcheng LiYan YuWenjun LiBo LiuXudong JiaoXinyu SongChangjun LvSong QinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chengcheng Li Yan Yu Wenjun Li Bo Liu Xudong Jiao Xinyu Song Changjun Lv Song Qin Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway
description	Abstract Our aim was to investigate the effects of phycocyanin (PC) on bleomycin (BLM)-induced pulmonary fibrosis (PF). In this study, C57 BL/6 wild-type (WT) mice and toll-like receptor (TLR) 2 deficient mice were treated with PC for 28 days following BLM exposure. Serum and lung tissues were collected on days 3, 7 and 28. Data shows PC significantly decreased the levels of hydroxyproline (HYP), vimentin, surfactant-associated protein C (SP-C), fibroblast specific protein-1 (S100A4) and α-smooth muscle actin (α-SMA) but dramatically increased E-cadherin and podoplanin (PDPN) expression on day 28. Moreover, PC greatly decreased the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and myeloperoxidase (MPO) at the earlier time. Reduced expression of key genes in the TLR2 pathway was also detected. Compared with WT mice, TLR2-deficient mice exhibited less injury, and the protective effect of PC was partly diminished in this background. These data indicate the anti-fibrotic effects of PC may be mediated by reducing W/D ratio, MPO, IL-6, TNF-α, protecting type I alveolar epithelial cells, inhibiting fibroblast proliferation, attenuating epithelial-mesenchymal transitions (EMT) and reducing oxidative stress. The TLR2-MyD88-NF-κB pathway plays an important role in PC-mediated reduction in pulmonary fibrosis.
format	article
author	Chengcheng Li Yan Yu Wenjun Li Bo Liu Xudong Jiao Xinyu Song Changjun Lv Song Qin
author_facet	Chengcheng Li Yan Yu Wenjun Li Bo Liu Xudong Jiao Xinyu Song Changjun Lv Song Qin
author_sort	Chengcheng Li
title	Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway
title_short	Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway
title_full	Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway
title_fullStr	Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway
title_full_unstemmed	Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway
title_sort	phycocyanin attenuates pulmonary fibrosis via the tlr2-myd88-nf-κb signaling pathway
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/09c77f351b4d4181b4675adfb5cd1473
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_version_	1718395502467219456

Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway

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