Intercellular bridges in vertebrate gastrulation.

The developing zebrafish embryo has been the subject of many studies of regional patterning, stereotypical cell movements and changes in cell shape. To better study the morphological features of cells during gastrulation, we generated mosaic embryos expressing membrane attached Dendra2 to highlight...

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Autores principales:	Luca Caneparo, Periklis Pantazis, William Dempsey, Scott E Fraser
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/09ca4cf5671943e8b91bb818570a0a87
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spelling	oai:doaj.org-article:09ca4cf5671943e8b91bb818570a0a872021-11-18T06:53:14ZIntercellular bridges in vertebrate gastrulation.1932-620310.1371/journal.pone.0020230https://doaj.org/article/09ca4cf5671943e8b91bb818570a0a872011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21647454/?tool=EBIhttps://doaj.org/toc/1932-6203The developing zebrafish embryo has been the subject of many studies of regional patterning, stereotypical cell movements and changes in cell shape. To better study the morphological features of cells during gastrulation, we generated mosaic embryos expressing membrane attached Dendra2 to highlight cellular boundaries. We find that intercellular bridges join a significant fraction of epiblast cells in the zebrafish embryo, reaching several cell diameters in length and spanning across different regions of the developing embryos. These intercellular bridges are distinct from the cellular protrusions previously reported as extending from hypoblast cells (1-2 cellular diameters in length) or epiblast cells (which were shorter). Most of the intercellular bridges were formed at pre-gastrula stages by the daughters of a dividing cell maintaining a membrane tether as they move apart after mitosis. These intercellular bridges persist during gastrulation and can mediate the transfer of proteins between distant cells. These findings reveal a surprising feature of the cellular landscape in zebrafish embryos and open new possibilities for cell-cell communication during gastrulation, with implications for modeling, cellular mechanics, and morphogenetic signaling.Luca CaneparoPeriklis PantazisWilliam DempseyScott E FraserPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e20230 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luca Caneparo Periklis Pantazis William Dempsey Scott E Fraser Intercellular bridges in vertebrate gastrulation.
description	The developing zebrafish embryo has been the subject of many studies of regional patterning, stereotypical cell movements and changes in cell shape. To better study the morphological features of cells during gastrulation, we generated mosaic embryos expressing membrane attached Dendra2 to highlight cellular boundaries. We find that intercellular bridges join a significant fraction of epiblast cells in the zebrafish embryo, reaching several cell diameters in length and spanning across different regions of the developing embryos. These intercellular bridges are distinct from the cellular protrusions previously reported as extending from hypoblast cells (1-2 cellular diameters in length) or epiblast cells (which were shorter). Most of the intercellular bridges were formed at pre-gastrula stages by the daughters of a dividing cell maintaining a membrane tether as they move apart after mitosis. These intercellular bridges persist during gastrulation and can mediate the transfer of proteins between distant cells. These findings reveal a surprising feature of the cellular landscape in zebrafish embryos and open new possibilities for cell-cell communication during gastrulation, with implications for modeling, cellular mechanics, and morphogenetic signaling.
format	article
author	Luca Caneparo Periklis Pantazis William Dempsey Scott E Fraser
author_facet	Luca Caneparo Periklis Pantazis William Dempsey Scott E Fraser
author_sort	Luca Caneparo
title	Intercellular bridges in vertebrate gastrulation.
title_short	Intercellular bridges in vertebrate gastrulation.
title_full	Intercellular bridges in vertebrate gastrulation.
title_fullStr	Intercellular bridges in vertebrate gastrulation.
title_full_unstemmed	Intercellular bridges in vertebrate gastrulation.
title_sort	intercellular bridges in vertebrate gastrulation.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/09ca4cf5671943e8b91bb818570a0a87
work_keys_str_mv	AT lucacaneparo intercellularbridgesinvertebrategastrulation AT periklispantazis intercellularbridgesinvertebrategastrulation AT williamdempsey intercellularbridgesinvertebrategastrulation AT scottefraser intercellularbridgesinvertebrategastrulation
_version_	1718424210007654400

Intercellular bridges in vertebrate gastrulation.

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