Combined strategy of endothelial cells coating, Sertoli cells coculture and infusion improves vascularization and rejection protection of islet graft.

Improving islet graft revascularization and inhibiting rejection become crucial tasks for prolonging islet graft survival. Endothelial cells (ECs) are the basis of islet vascularization and Sertoli cells (SCs) have the talent to provide nutritional support and exert immunosuppressive effects. We con...

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Autores principales:	Yang Li, Wujun Xue, Hongbao Liu, Ping Fan, Xiaohong Wang, Xiaoming Ding, Xiaohui Tian, Xinshun Feng, Xiaoming Pan, Jin Zheng, Puxun Tian, Chenguang Ding, Xiaohu Fan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/09d1c88d0014468b995a9cb475abc8cb
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spelling	oai:doaj.org-article:09d1c88d0014468b995a9cb475abc8cb2021-11-18T07:56:48ZCombined strategy of endothelial cells coating, Sertoli cells coculture and infusion improves vascularization and rejection protection of islet graft.1932-620310.1371/journal.pone.0056696https://doaj.org/article/09d1c88d0014468b995a9cb475abc8cb2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23437215/?tool=EBIhttps://doaj.org/toc/1932-6203Improving islet graft revascularization and inhibiting rejection become crucial tasks for prolonging islet graft survival. Endothelial cells (ECs) are the basis of islet vascularization and Sertoli cells (SCs) have the talent to provide nutritional support and exert immunosuppressive effects. We construct a combined strategy of ECs coating in the presence of nutritious and immune factors supplied by SCs in a co-culture system to investigate the effect of vascularization and rejection inhibition for islet graft. In vivo, the combined strategy improved the survival and vascularization as well as inhibited lymphocytes and inflammatory cytokines. In vitro, we found the combinatorial strategy improved the function of islets and the effect of ECs-coating on islets. Combined strategy treated islets revealed higher levels of anti-apoptotic signal molecules (Bcl-2 and HSP-32), survival and function related molecules (PDX-1, Ki-67, ERK1/2 and Akt) and demonstrated increased vascular endothelial growth factor receptor 2 (KDR) and angiogenesis signal molecules (FAk and PLC-γ). SCs effectively inhibited the activation of lymphocyte stimulated by islets and ECs. Predominantly immunosuppressive cytokines could be detected in culture supernatants of the SCs coculture group. These results suggest that ECs-coating and Sertoli cells co-culture or infusion synergistically enhance islet survival and function after transplantation.Yang LiWujun XueHongbao LiuPing FanXiaohong WangXiaoming DingXiaohui TianXinshun FengXiaoming PanJin ZhengPuxun TianChenguang DingXiaohu FanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e56696 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yang Li Wujun Xue Hongbao Liu Ping Fan Xiaohong Wang Xiaoming Ding Xiaohui Tian Xinshun Feng Xiaoming Pan Jin Zheng Puxun Tian Chenguang Ding Xiaohu Fan Combined strategy of endothelial cells coating, Sertoli cells coculture and infusion improves vascularization and rejection protection of islet graft.
description	Improving islet graft revascularization and inhibiting rejection become crucial tasks for prolonging islet graft survival. Endothelial cells (ECs) are the basis of islet vascularization and Sertoli cells (SCs) have the talent to provide nutritional support and exert immunosuppressive effects. We construct a combined strategy of ECs coating in the presence of nutritious and immune factors supplied by SCs in a co-culture system to investigate the effect of vascularization and rejection inhibition for islet graft. In vivo, the combined strategy improved the survival and vascularization as well as inhibited lymphocytes and inflammatory cytokines. In vitro, we found the combinatorial strategy improved the function of islets and the effect of ECs-coating on islets. Combined strategy treated islets revealed higher levels of anti-apoptotic signal molecules (Bcl-2 and HSP-32), survival and function related molecules (PDX-1, Ki-67, ERK1/2 and Akt) and demonstrated increased vascular endothelial growth factor receptor 2 (KDR) and angiogenesis signal molecules (FAk and PLC-γ). SCs effectively inhibited the activation of lymphocyte stimulated by islets and ECs. Predominantly immunosuppressive cytokines could be detected in culture supernatants of the SCs coculture group. These results suggest that ECs-coating and Sertoli cells co-culture or infusion synergistically enhance islet survival and function after transplantation.
format	article
author	Yang Li Wujun Xue Hongbao Liu Ping Fan Xiaohong Wang Xiaoming Ding Xiaohui Tian Xinshun Feng Xiaoming Pan Jin Zheng Puxun Tian Chenguang Ding Xiaohu Fan
author_facet	Yang Li Wujun Xue Hongbao Liu Ping Fan Xiaohong Wang Xiaoming Ding Xiaohui Tian Xinshun Feng Xiaoming Pan Jin Zheng Puxun Tian Chenguang Ding Xiaohu Fan
author_sort	Yang Li
title	Combined strategy of endothelial cells coating, Sertoli cells coculture and infusion improves vascularization and rejection protection of islet graft.
title_short	Combined strategy of endothelial cells coating, Sertoli cells coculture and infusion improves vascularization and rejection protection of islet graft.
title_full	Combined strategy of endothelial cells coating, Sertoli cells coculture and infusion improves vascularization and rejection protection of islet graft.
title_fullStr	Combined strategy of endothelial cells coating, Sertoli cells coculture and infusion improves vascularization and rejection protection of islet graft.
title_full_unstemmed	Combined strategy of endothelial cells coating, Sertoli cells coculture and infusion improves vascularization and rejection protection of islet graft.
title_sort	combined strategy of endothelial cells coating, sertoli cells coculture and infusion improves vascularization and rejection protection of islet graft.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/09d1c88d0014468b995a9cb475abc8cb
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Combined strategy of endothelial cells coating, Sertoli cells coculture and infusion improves vascularization and rejection protection of islet graft.

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