A comprehensive approach to stool donor screening for faecal microbiota transplantation in China
Abstract Background Faecal microbiota transplantation (FMT) is an effective therapy for recurrent Clostridium difficile infections and chronic gastrointestional infections. However, the risks of FMT and the selection process of suitable donors remain insufficiently characterized. The eligibility rat...
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2021
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oai:doaj.org-article:09d4590655a9496783fde1dcf7a95c392021-11-28T12:39:07ZA comprehensive approach to stool donor screening for faecal microbiota transplantation in China10.1186/s12934-021-01705-01475-2859https://doaj.org/article/09d4590655a9496783fde1dcf7a95c392021-11-01T00:00:00Zhttps://doi.org/10.1186/s12934-021-01705-0https://doaj.org/toc/1475-2859Abstract Background Faecal microbiota transplantation (FMT) is an effective therapy for recurrent Clostridium difficile infections and chronic gastrointestional infections. However, the risks of FMT and the selection process of suitable donors remain insufficiently characterized. The eligibility rate for screening, underlying microbial basis, and core ethical issues of stool donors for FMT are yet to be elucidated in China. Results The potential stool donors were screened from December 2017 to December 2019 with the help of an online survey, clinical assessments, and stool and blood testing. Bioinformatics analyses were performed, and the composition and stability of gut microbiota in stool obtained from eligible donors were dynamically observed using metagenomics. Meanwhile, we build a donor microbial evaluation index (DoMEI) for stool donor screening. In the screening process, we also focused on ethical principles and requirements. Of the 2071 participants, 66 donors were selected via the screening process (3.19% success rate). Although there were significant differences in gut microbiota among donors, we found that the changes in the gut microbiota of the same donor were typically more stable than those between donors over time. Conclusions DoMEI provides a potential reference index for regular stool donor re-evaluation. In this retrospective study, we summarised the donor recruitment and screening procedure ensuring the safety and tolerability for FMT in China. Based on the latest advances in this field, we carried out rigorous recommendation and method which can assist stool bank and clinicians to screen eligible stool donor for FMT.Jianquan HeXingxiang HeYonghui MaLuxi YangHaiming FangShu ShangHuping XiaGuanghui LianHailing TangQizhi WangJunping WangZhihui LinJianbo WenYuedong LiuChunbao ZhaiWen WangXueliang JiangJi XuanMorong LiuShiyun LuXuejun LiHan WangCong OuyangMan CaoAiqiang LinBangzhou ZhangDepei WuYe ChenChuanxing XiaoBMCarticleFaecal microbiota transplantationMicrobiota evaluationDonor selectionMetagenomicsEthical issueMicrobiologyQR1-502ENMicrobial Cell Factories, Vol 20, Iss 1, Pp 1-14 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Faecal microbiota transplantation Microbiota evaluation Donor selection Metagenomics Ethical issue Microbiology QR1-502 |
| spellingShingle |
Faecal microbiota transplantation Microbiota evaluation Donor selection Metagenomics Ethical issue Microbiology QR1-502 Jianquan He Xingxiang He Yonghui Ma Luxi Yang Haiming Fang Shu Shang Huping Xia Guanghui Lian Hailing Tang Qizhi Wang Junping Wang Zhihui Lin Jianbo Wen Yuedong Liu Chunbao Zhai Wen Wang Xueliang Jiang Ji Xuan Morong Liu Shiyun Lu Xuejun Li Han Wang Cong Ouyang Man Cao Aiqiang Lin Bangzhou Zhang Depei Wu Ye Chen Chuanxing Xiao A comprehensive approach to stool donor screening for faecal microbiota transplantation in China |
| description |
Abstract Background Faecal microbiota transplantation (FMT) is an effective therapy for recurrent Clostridium difficile infections and chronic gastrointestional infections. However, the risks of FMT and the selection process of suitable donors remain insufficiently characterized. The eligibility rate for screening, underlying microbial basis, and core ethical issues of stool donors for FMT are yet to be elucidated in China. Results The potential stool donors were screened from December 2017 to December 2019 with the help of an online survey, clinical assessments, and stool and blood testing. Bioinformatics analyses were performed, and the composition and stability of gut microbiota in stool obtained from eligible donors were dynamically observed using metagenomics. Meanwhile, we build a donor microbial evaluation index (DoMEI) for stool donor screening. In the screening process, we also focused on ethical principles and requirements. Of the 2071 participants, 66 donors were selected via the screening process (3.19% success rate). Although there were significant differences in gut microbiota among donors, we found that the changes in the gut microbiota of the same donor were typically more stable than those between donors over time. Conclusions DoMEI provides a potential reference index for regular stool donor re-evaluation. In this retrospective study, we summarised the donor recruitment and screening procedure ensuring the safety and tolerability for FMT in China. Based on the latest advances in this field, we carried out rigorous recommendation and method which can assist stool bank and clinicians to screen eligible stool donor for FMT. |
| format |
article |
| author |
Jianquan He Xingxiang He Yonghui Ma Luxi Yang Haiming Fang Shu Shang Huping Xia Guanghui Lian Hailing Tang Qizhi Wang Junping Wang Zhihui Lin Jianbo Wen Yuedong Liu Chunbao Zhai Wen Wang Xueliang Jiang Ji Xuan Morong Liu Shiyun Lu Xuejun Li Han Wang Cong Ouyang Man Cao Aiqiang Lin Bangzhou Zhang Depei Wu Ye Chen Chuanxing Xiao |
| author_facet |
Jianquan He Xingxiang He Yonghui Ma Luxi Yang Haiming Fang Shu Shang Huping Xia Guanghui Lian Hailing Tang Qizhi Wang Junping Wang Zhihui Lin Jianbo Wen Yuedong Liu Chunbao Zhai Wen Wang Xueliang Jiang Ji Xuan Morong Liu Shiyun Lu Xuejun Li Han Wang Cong Ouyang Man Cao Aiqiang Lin Bangzhou Zhang Depei Wu Ye Chen Chuanxing Xiao |
| author_sort |
Jianquan He |
| title |
A comprehensive approach to stool donor screening for faecal microbiota transplantation in China |
| title_short |
A comprehensive approach to stool donor screening for faecal microbiota transplantation in China |
| title_full |
A comprehensive approach to stool donor screening for faecal microbiota transplantation in China |
| title_fullStr |
A comprehensive approach to stool donor screening for faecal microbiota transplantation in China |
| title_full_unstemmed |
A comprehensive approach to stool donor screening for faecal microbiota transplantation in China |
| title_sort |
comprehensive approach to stool donor screening for faecal microbiota transplantation in china |
| publisher |
BMC |
| publishDate |
2021 |
| url |
https://doaj.org/article/09d4590655a9496783fde1dcf7a95c39 |
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