Dietary β-Carotene on Postpartum Uterine Recovery in Mice: Crosstalk Between Gut Microbiota and Inflammation

As the precursor of vitamin A, β-carotene has a positive effect on reproductive performance. Our previous study has shown that β-carotene can increase antioxidant enzyme activity potentially through regulating gut microbiota in pregnant sows. This study aimed to clarify the effect of β-carotene on r...

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Autores principales:	Xizi Yang, Ziyu He, Ruizhi Hu, Jiahao Yan, Qianjin Zhang, Baizhen Li, Xupeng Yuan, Hongfu Zhang, Jianhua He, Shusong Wu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	β-carotene postpartum uterine recovery gut microbiota inflammation reproductive performance Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/09d4b6605f7a48a397d4de832c334844
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spelling	oai:doaj.org-article:09d4b6605f7a48a397d4de832c3348442021-11-30T18:35:32ZDietary β-Carotene on Postpartum Uterine Recovery in Mice: Crosstalk Between Gut Microbiota and Inflammation1664-322410.3389/fimmu.2021.744425https://doaj.org/article/09d4b6605f7a48a397d4de832c3348442021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.744425/fullhttps://doaj.org/toc/1664-3224As the precursor of vitamin A, β-carotene has a positive effect on reproductive performance. Our previous study has shown that β-carotene can increase antioxidant enzyme activity potentially through regulating gut microbiota in pregnant sows. This study aimed to clarify the effect of β-carotene on reproductive performance and postpartum uterine recovery from the aspect of inflammation and gut microbiota by using a mouse model. Twenty-seven 6 weeks old female Kunming mice were randomly assigned into 3 groups (n=9), and fed with a diet containing 0, 30 or 90 mg/kg β-carotene, respectively. The results showed that dietary supplementation of β-carotene reduced postpartum uterine hyperemia and uterine mass index (P<0.05), improved intestinal villus height and villus height to crypt depth ratio, decreased serum TNF-α and IL-4 concentration (P<0.05), while no differences were observed in litter size and litter weight among three treatments. Characterization of gut microbiota revealed that β-carotene up-regulated the relative abundance of genera Akkermansia, Candidatus Stoquefichus and Faecalibaculum, but down-regulated the relative abundance of Alloprevotella and Helicobacter. Correlation analysis revealed that Akkermansia was negatively correlated with the IL-4 concentration, while Candidatus Stoquefichus and Faecalibaculum had a negative linear correlation with both TNF-α and IL-4 concentration. On the other hand, Alloprevotella was positively correlated with the TNF-α, and Helicobacter had a positive correlation with both TNF-α and IL-4 concentration. These data demonstrated that dietary supplementation of β-carotene contributes to postpartum uterine recovery by decreasing postpartum uterine hemorrhage and inhibiting the production of inflammatory cytokines potentially through modulating gut microbiota.Xizi YangZiyu HeRuizhi HuJiahao YanQianjin ZhangBaizhen LiXupeng YuanHongfu ZhangJianhua HeShusong WuFrontiers Media S.A.articleβ-carotenepostpartum uterine recoverygut microbiotainflammationreproductive performanceImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	β-carotene postpartum uterine recovery gut microbiota inflammation reproductive performance Immunologic diseases. Allergy RC581-607
spellingShingle	β-carotene postpartum uterine recovery gut microbiota inflammation reproductive performance Immunologic diseases. Allergy RC581-607 Xizi Yang Ziyu He Ruizhi Hu Jiahao Yan Qianjin Zhang Baizhen Li Xupeng Yuan Hongfu Zhang Jianhua He Shusong Wu Dietary β-Carotene on Postpartum Uterine Recovery in Mice: Crosstalk Between Gut Microbiota and Inflammation
description	As the precursor of vitamin A, β-carotene has a positive effect on reproductive performance. Our previous study has shown that β-carotene can increase antioxidant enzyme activity potentially through regulating gut microbiota in pregnant sows. This study aimed to clarify the effect of β-carotene on reproductive performance and postpartum uterine recovery from the aspect of inflammation and gut microbiota by using a mouse model. Twenty-seven 6 weeks old female Kunming mice were randomly assigned into 3 groups (n=9), and fed with a diet containing 0, 30 or 90 mg/kg β-carotene, respectively. The results showed that dietary supplementation of β-carotene reduced postpartum uterine hyperemia and uterine mass index (P<0.05), improved intestinal villus height and villus height to crypt depth ratio, decreased serum TNF-α and IL-4 concentration (P<0.05), while no differences were observed in litter size and litter weight among three treatments. Characterization of gut microbiota revealed that β-carotene up-regulated the relative abundance of genera Akkermansia, Candidatus Stoquefichus and Faecalibaculum, but down-regulated the relative abundance of Alloprevotella and Helicobacter. Correlation analysis revealed that Akkermansia was negatively correlated with the IL-4 concentration, while Candidatus Stoquefichus and Faecalibaculum had a negative linear correlation with both TNF-α and IL-4 concentration. On the other hand, Alloprevotella was positively correlated with the TNF-α, and Helicobacter had a positive correlation with both TNF-α and IL-4 concentration. These data demonstrated that dietary supplementation of β-carotene contributes to postpartum uterine recovery by decreasing postpartum uterine hemorrhage and inhibiting the production of inflammatory cytokines potentially through modulating gut microbiota.
format	article
author	Xizi Yang Ziyu He Ruizhi Hu Jiahao Yan Qianjin Zhang Baizhen Li Xupeng Yuan Hongfu Zhang Jianhua He Shusong Wu
author_facet	Xizi Yang Ziyu He Ruizhi Hu Jiahao Yan Qianjin Zhang Baizhen Li Xupeng Yuan Hongfu Zhang Jianhua He Shusong Wu
author_sort	Xizi Yang
title	Dietary β-Carotene on Postpartum Uterine Recovery in Mice: Crosstalk Between Gut Microbiota and Inflammation
title_short	Dietary β-Carotene on Postpartum Uterine Recovery in Mice: Crosstalk Between Gut Microbiota and Inflammation
title_full	Dietary β-Carotene on Postpartum Uterine Recovery in Mice: Crosstalk Between Gut Microbiota and Inflammation
title_fullStr	Dietary β-Carotene on Postpartum Uterine Recovery in Mice: Crosstalk Between Gut Microbiota and Inflammation
title_full_unstemmed	Dietary β-Carotene on Postpartum Uterine Recovery in Mice: Crosstalk Between Gut Microbiota and Inflammation
title_sort	dietary β-carotene on postpartum uterine recovery in mice: crosstalk between gut microbiota and inflammation
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/09d4b6605f7a48a397d4de832c334844
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Dietary β-Carotene on Postpartum Uterine Recovery in Mice: Crosstalk Between Gut Microbiota and Inflammation

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