Anti-Inflammatory Activity and Chemical Analysis of Different Fractions from Solidago chilensis Inflorescence

Solidago chilensis Meyen (Compositae) is a species native to South America (Brazil) popularly known as arnica. In Brazilian popular medicine, inflorescences and rhizomes of this plant have been used since the end of the 19th century to replace the exogenous and hepatotoxic Arnica montana L. in the t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Thais Morais de Brito, Fabio Coelho Amendoeira, Temistocles Barroso de Oliveira, Laís Higino Doro, Esdras Barbosa Garcia, Naína Monsores Felix da Silva, Amanda da Silva Chaves, Flavia Fontenelle Muylaert, Tatiana Almeida Pádua, Elaine Cruz Rosas, Maria das Graças M. O. Henriques, Valber da Silva Frutuoso, Simone Sacramento Valverde, Fausto Klabund Ferraris
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Cytology QH573-671
Acceso en línea:	https://doaj.org/article/09d71b86892445d7ada1f75f10be5289
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:09d71b86892445d7ada1f75f10be5289
record_format	dspace
spelling	oai:doaj.org-article:09d71b86892445d7ada1f75f10be52892021-11-08T02:35:31ZAnti-Inflammatory Activity and Chemical Analysis of Different Fractions from Solidago chilensis Inflorescence1942-099410.1155/2021/7612380https://doaj.org/article/09d71b86892445d7ada1f75f10be52892021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/7612380https://doaj.org/toc/1942-0994Solidago chilensis Meyen (Compositae) is a species native to South America (Brazil) popularly known as arnica. In Brazilian popular medicine, inflorescences and rhizomes of this plant have been used since the end of the 19th century to replace the exogenous and hepatotoxic Arnica montana L. in the treatment of edema and inflammatory pathologies. Although the anti-inflammatory activity of S. chilensis is evidenced in the literature, there is a lack of studies with enriched fractions or compounds isolated from it. The objective of the current study was to characterize phytochemically and to evaluate the pharmacological action in vivo and in vitro of the crude extract and the different fractions (hexane, dichloromethane, acetal, butanolic, and aqueous) isolated from the inflorescence of S. chilensis. The inflorescence crude extract (ScIE) and fractions were administered by intraperitoneal route to mice at different doses. In an LPS-induced pleurisy model, inhibition of leukocyte influx was observed for the ScIE and all fractions tested, as compared to controls. Dichloromethane (ScDicF), butanolic (ScButF), and aqueous (ScAquF) were selected for further analysis as they showed the best inhibitory effects in leukocyte migration and inflammatory cytokine and chemokine production: TNF-α, CXCL1/KC, CXCL2/MIP-2, and CCL11/eotaxin-1. In LPS-stimulated J774A.1 cell line, ScIE and the ScDicF exhibited an inhibitory effect on nitric oxide (NO) production and downmodulated the COX-2 expression; ScAquF failed to modulate NO production and COX-2 expression. In phytochemical analysis, HPLC-UV-DAD chromatograms of ScDicF and ScAquF showed the main peaks with UV spectrum characteristics of flavonoids; chlorogenic acid and isoquercetin were the most present phytochemicals identified in the ScAquF, and a high number of n-alkanes was found in ScHexF. Our study was the first to address biological effects and correlate them to phytochemically characterized fractions from inflorescences of S. chilensis.Thais Morais de BritoFabio Coelho AmendoeiraTemistocles Barroso de OliveiraLaís Higino DoroEsdras Barbosa GarciaNaína Monsores Felix da SilvaAmanda da Silva ChavesFlavia Fontenelle MuylaertTatiana Almeida PáduaElaine Cruz RosasMaria das Graças M. O. HenriquesValber da Silva FrutuosoSimone Sacramento ValverdeFausto Klabund FerrarisHindawi LimitedarticleCytologyQH573-671ENOxidative Medicine and Cellular Longevity, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cytology QH573-671
spellingShingle	Cytology QH573-671 Thais Morais de Brito Fabio Coelho Amendoeira Temistocles Barroso de Oliveira Laís Higino Doro Esdras Barbosa Garcia Naína Monsores Felix da Silva Amanda da Silva Chaves Flavia Fontenelle Muylaert Tatiana Almeida Pádua Elaine Cruz Rosas Maria das Graças M. O. Henriques Valber da Silva Frutuoso Simone Sacramento Valverde Fausto Klabund Ferraris Anti-Inflammatory Activity and Chemical Analysis of Different Fractions from Solidago chilensis Inflorescence
description	Solidago chilensis Meyen (Compositae) is a species native to South America (Brazil) popularly known as arnica. In Brazilian popular medicine, inflorescences and rhizomes of this plant have been used since the end of the 19th century to replace the exogenous and hepatotoxic Arnica montana L. in the treatment of edema and inflammatory pathologies. Although the anti-inflammatory activity of S. chilensis is evidenced in the literature, there is a lack of studies with enriched fractions or compounds isolated from it. The objective of the current study was to characterize phytochemically and to evaluate the pharmacological action in vivo and in vitro of the crude extract and the different fractions (hexane, dichloromethane, acetal, butanolic, and aqueous) isolated from the inflorescence of S. chilensis. The inflorescence crude extract (ScIE) and fractions were administered by intraperitoneal route to mice at different doses. In an LPS-induced pleurisy model, inhibition of leukocyte influx was observed for the ScIE and all fractions tested, as compared to controls. Dichloromethane (ScDicF), butanolic (ScButF), and aqueous (ScAquF) were selected for further analysis as they showed the best inhibitory effects in leukocyte migration and inflammatory cytokine and chemokine production: TNF-α, CXCL1/KC, CXCL2/MIP-2, and CCL11/eotaxin-1. In LPS-stimulated J774A.1 cell line, ScIE and the ScDicF exhibited an inhibitory effect on nitric oxide (NO) production and downmodulated the COX-2 expression; ScAquF failed to modulate NO production and COX-2 expression. In phytochemical analysis, HPLC-UV-DAD chromatograms of ScDicF and ScAquF showed the main peaks with UV spectrum characteristics of flavonoids; chlorogenic acid and isoquercetin were the most present phytochemicals identified in the ScAquF, and a high number of n-alkanes was found in ScHexF. Our study was the first to address biological effects and correlate them to phytochemically characterized fractions from inflorescences of S. chilensis.
format	article
author	Thais Morais de Brito Fabio Coelho Amendoeira Temistocles Barroso de Oliveira Laís Higino Doro Esdras Barbosa Garcia Naína Monsores Felix da Silva Amanda da Silva Chaves Flavia Fontenelle Muylaert Tatiana Almeida Pádua Elaine Cruz Rosas Maria das Graças M. O. Henriques Valber da Silva Frutuoso Simone Sacramento Valverde Fausto Klabund Ferraris
author_facet	Thais Morais de Brito Fabio Coelho Amendoeira Temistocles Barroso de Oliveira Laís Higino Doro Esdras Barbosa Garcia Naína Monsores Felix da Silva Amanda da Silva Chaves Flavia Fontenelle Muylaert Tatiana Almeida Pádua Elaine Cruz Rosas Maria das Graças M. O. Henriques Valber da Silva Frutuoso Simone Sacramento Valverde Fausto Klabund Ferraris
author_sort	Thais Morais de Brito
title	Anti-Inflammatory Activity and Chemical Analysis of Different Fractions from Solidago chilensis Inflorescence
title_short	Anti-Inflammatory Activity and Chemical Analysis of Different Fractions from Solidago chilensis Inflorescence
title_full	Anti-Inflammatory Activity and Chemical Analysis of Different Fractions from Solidago chilensis Inflorescence
title_fullStr	Anti-Inflammatory Activity and Chemical Analysis of Different Fractions from Solidago chilensis Inflorescence
title_full_unstemmed	Anti-Inflammatory Activity and Chemical Analysis of Different Fractions from Solidago chilensis Inflorescence
title_sort	anti-inflammatory activity and chemical analysis of different fractions from solidago chilensis inflorescence
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/09d71b86892445d7ada1f75f10be5289
work_keys_str_mv	AT thaismoraisdebrito antiinflammatoryactivityandchemicalanalysisofdifferentfractionsfromsolidagochilensisinflorescence AT fabiocoelhoamendoeira antiinflammatoryactivityandchemicalanalysisofdifferentfractionsfromsolidagochilensisinflorescence AT temistoclesbarrosodeoliveira antiinflammatoryactivityandchemicalanalysisofdifferentfractionsfromsolidagochilensisinflorescence AT laishiginodoro antiinflammatoryactivityandchemicalanalysisofdifferentfractionsfromsolidagochilensisinflorescence AT esdrasbarbosagarcia antiinflammatoryactivityandchemicalanalysisofdifferentfractionsfromsolidagochilensisinflorescence AT nainamonsoresfelixdasilva antiinflammatoryactivityandchemicalanalysisofdifferentfractionsfromsolidagochilensisinflorescence AT amandadasilvachaves antiinflammatoryactivityandchemicalanalysisofdifferentfractionsfromsolidagochilensisinflorescence AT flaviafontenellemuylaert antiinflammatoryactivityandchemicalanalysisofdifferentfractionsfromsolidagochilensisinflorescence AT tatianaalmeidapadua antiinflammatoryactivityandchemicalanalysisofdifferentfractionsfromsolidagochilensisinflorescence AT elainecruzrosas antiinflammatoryactivityandchemicalanalysisofdifferentfractionsfromsolidagochilensisinflorescence AT mariadasgracasmohenriques antiinflammatoryactivityandchemicalanalysisofdifferentfractionsfromsolidagochilensisinflorescence AT valberdasilvafrutuoso antiinflammatoryactivityandchemicalanalysisofdifferentfractionsfromsolidagochilensisinflorescence AT simonesacramentovalverde antiinflammatoryactivityandchemicalanalysisofdifferentfractionsfromsolidagochilensisinflorescence AT faustoklabundferraris antiinflammatoryactivityandchemicalanalysisofdifferentfractionsfromsolidagochilensisinflorescence
_version_	1718443159347789824

Anti-Inflammatory Activity and Chemical Analysis of Different Fractions from Solidago chilensis Inflorescence

Ejemplares similares