Structural characterization of microcrystalline and nanocrystalline cellulose from Ananas comosus L. leaves: Cytocompatibility and molecular docking studies

The present study focused on the preparation of microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC) from pineapple (Ananas comosus L.) leaves using chemical treatments followed by acid hydrolysis. Pineapple leaves could be used in medical applications such as drug delivery carriers....

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Autores principales:	Sainorudin Muhammad Hanif, Abdullah Nur Athirah, Asmal Rani Mohd Saiful, Mohammad Masita, Mahizan Munirah, Shadan Nursyazwani, Abd Kadir Nurul Huda, Yaakob Zahira, El-Denglawey Adel, Alam Mahboob
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	cytotoxicity mcc ncc pineapple leaves molecular docking Technology T Chemical technology TP1-1185 Physical and theoretical chemistry QD450-801
Acceso en línea:	https://doaj.org/article/09e340af5b8a4af8a4cb40988df9ab81
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spelling	oai:doaj.org-article:09e340af5b8a4af8a4cb40988df9ab812021-12-05T14:10:57ZStructural characterization of microcrystalline and nanocrystalline cellulose from Ananas comosus L. leaves: Cytocompatibility and molecular docking studies2191-909710.1515/ntrev-2021-0053https://doaj.org/article/09e340af5b8a4af8a4cb40988df9ab812021-08-01T00:00:00Zhttps://doi.org/10.1515/ntrev-2021-0053https://doaj.org/toc/2191-9097The present study focused on the preparation of microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC) from pineapple (Ananas comosus L.) leaves using chemical treatments followed by acid hydrolysis. Pineapple leaves could be used in medical applications such as drug delivery carriers. Advanced spectroscopy techniques such as Fourier-transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) were used to analyze the physical, chemical, and morphological features of the isolated MCC and NCC; the results indicated the needle-shaped form of nanostructures with good purity and high crystallinity index of 75.00 and 76.38%, respectively. In addition, inhibition of the treated MRC-5 cells with all the samples revealed that the percentage of cell viability was less than 30%, which is an interesting finding given their role in the cytotoxicity effect of MCC and NCC. It appears that MCC and NCC derived from pineapple leaves have lower toxicity. As a result, the developed MCC and NCC can be used in pharmaceutical applications as a novel drug delivery system. Molecular docking was performed to understand the non-bonding interaction of cellulose with human acid-beta-glucosidase (β-Glc) (PDB: 1OGS). The docking result shows that cellulose unit docked within the active pocket of the enzyme by forming hydrogen bonds against ASN19, THR21, and VAL17 with distances of 2.18, 1.93, and 2.92 Å, respectively, with binding energy (−5.0 kcal/mol) resulting in close interaction of cellulose unit with the receptor.Sainorudin Muhammad HanifAbdullah Nur AthirahAsmal Rani Mohd SaifulMohammad MasitaMahizan MunirahShadan NursyazwaniAbd Kadir Nurul HudaYaakob ZahiraEl-Denglawey AdelAlam MahboobDe Gruyterarticlecytotoxicitymccnccpineapple leavesmolecular dockingTechnologyTChemical technologyTP1-1185Physical and theoretical chemistryQD450-801ENNanotechnology Reviews, Vol 10, Iss 1, Pp 793-806 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cytotoxicity mcc ncc pineapple leaves molecular docking Technology T Chemical technology TP1-1185 Physical and theoretical chemistry QD450-801
spellingShingle	cytotoxicity mcc ncc pineapple leaves molecular docking Technology T Chemical technology TP1-1185 Physical and theoretical chemistry QD450-801 Sainorudin Muhammad Hanif Abdullah Nur Athirah Asmal Rani Mohd Saiful Mohammad Masita Mahizan Munirah Shadan Nursyazwani Abd Kadir Nurul Huda Yaakob Zahira El-Denglawey Adel Alam Mahboob Structural characterization of microcrystalline and nanocrystalline cellulose from Ananas comosus L. leaves: Cytocompatibility and molecular docking studies
description	The present study focused on the preparation of microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC) from pineapple (Ananas comosus L.) leaves using chemical treatments followed by acid hydrolysis. Pineapple leaves could be used in medical applications such as drug delivery carriers. Advanced spectroscopy techniques such as Fourier-transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) were used to analyze the physical, chemical, and morphological features of the isolated MCC and NCC; the results indicated the needle-shaped form of nanostructures with good purity and high crystallinity index of 75.00 and 76.38%, respectively. In addition, inhibition of the treated MRC-5 cells with all the samples revealed that the percentage of cell viability was less than 30%, which is an interesting finding given their role in the cytotoxicity effect of MCC and NCC. It appears that MCC and NCC derived from pineapple leaves have lower toxicity. As a result, the developed MCC and NCC can be used in pharmaceutical applications as a novel drug delivery system. Molecular docking was performed to understand the non-bonding interaction of cellulose with human acid-beta-glucosidase (β-Glc) (PDB: 1OGS). The docking result shows that cellulose unit docked within the active pocket of the enzyme by forming hydrogen bonds against ASN19, THR21, and VAL17 with distances of 2.18, 1.93, and 2.92 Å, respectively, with binding energy (−5.0 kcal/mol) resulting in close interaction of cellulose unit with the receptor.
format	article
author	Sainorudin Muhammad Hanif Abdullah Nur Athirah Asmal Rani Mohd Saiful Mohammad Masita Mahizan Munirah Shadan Nursyazwani Abd Kadir Nurul Huda Yaakob Zahira El-Denglawey Adel Alam Mahboob
author_facet	Sainorudin Muhammad Hanif Abdullah Nur Athirah Asmal Rani Mohd Saiful Mohammad Masita Mahizan Munirah Shadan Nursyazwani Abd Kadir Nurul Huda Yaakob Zahira El-Denglawey Adel Alam Mahboob
author_sort	Sainorudin Muhammad Hanif
title	Structural characterization of microcrystalline and nanocrystalline cellulose from Ananas comosus L. leaves: Cytocompatibility and molecular docking studies
title_short	Structural characterization of microcrystalline and nanocrystalline cellulose from Ananas comosus L. leaves: Cytocompatibility and molecular docking studies
title_full	Structural characterization of microcrystalline and nanocrystalline cellulose from Ananas comosus L. leaves: Cytocompatibility and molecular docking studies
title_fullStr	Structural characterization of microcrystalline and nanocrystalline cellulose from Ananas comosus L. leaves: Cytocompatibility and molecular docking studies
title_full_unstemmed	Structural characterization of microcrystalline and nanocrystalline cellulose from Ananas comosus L. leaves: Cytocompatibility and molecular docking studies
title_sort	structural characterization of microcrystalline and nanocrystalline cellulose from ananas comosus l. leaves: cytocompatibility and molecular docking studies
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/09e340af5b8a4af8a4cb40988df9ab81
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Structural characterization of microcrystalline and nanocrystalline cellulose from Ananas comosus L. leaves: Cytocompatibility and molecular docking studies

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