Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection

Anna Meresta,1 Justyna Folkert,1 Timo Gaber,2 Korneliusz Miksch,1 Frank Buttgereit,2 Jacqueline Detert,2 Nicole Pischon,3,* Katarzyna Gurzawska3,4,* 1Environmental Biotechnology Department, Faculty of Power and Environmental, Silesian University of Technology, Gliwice, Poland; 2Department of Rheuma...

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Autores principales:	Meresta A, Folkert J, Gaber T, Miksch K, Buttgereit F, Detert J, Pischon N, Gurzawska K
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	nanocoatings Rhamnogalacturonan-I Porphyromonas gingivalis osteoblasts inflammation. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0a01db450bac42e986cd53bfe42001e8
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spelling	oai:doaj.org-article:0a01db450bac42e986cd53bfe42001e82021-12-02T01:01:57ZPlant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection1178-2013https://doaj.org/article/0a01db450bac42e986cd53bfe42001e82017-01-01T00:00:00Zhttps://www.dovepress.com/plant-derived-pectin-nanocoatings-to-prevent-inflammatory-cellular-res-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Anna Meresta,1 Justyna Folkert,1 Timo Gaber,2 Korneliusz Miksch,1 Frank Buttgereit,2 Jacqueline Detert,2 Nicole Pischon,3,* Katarzyna Gurzawska3,4,* 1Environmental Biotechnology Department, Faculty of Power and Environmental, Silesian University of Technology, Gliwice, Poland; 2Department of Rheumatology and Clinical Immunology, 3Department of Periodontology, Charité University Medicine, Berlin, Germany; 4Oral Surgery Department, The School of Dentistry, University of Birmingham, Birmingham, UK *These authors contributed equally to this work Background: Bioengineered plant-derived Rhamnogalacturonan-Is (RG-Is) from pectins are potential candidates for surface nanocoating of medical devices. It has recently been reported that RG-I nanocoatings may prevent bacterial infection and improve the biocompatibility of implants. The aim of the study was to evaluate in vitro impact of bioengineered RG-I nanocoatings on osteogenic capacity and proinflammatory cytokine response of murine osteoblasts following Porphyromonas gingivalis infection.Methods: Murine MC3T3-E1 osteoblasts and isolated primary calvarial osteoblasts from C57BL/6J (B6J osteoblasts) mice were infected with P. gingivalis and incubated on tissue culture polystyrene plates with or without nanocoatings of unmodified RG-Is isolated from potato pulps (PU) or dearabinanated RG-Is (PA). To investigate a behavior of infected osteoblasts cultured on RG-Is cell morphology, proliferation, metabolic activity, mineralization and osteogenic and pro-inflammatory gene expression were examined.Results: Following P. gingivalis infection, PA, but not PU, significantly promoted MC3T3-E1 and BJ6 osteoblasts proliferation, metabolic activity, and calcium deposition. Moreover, Il-1b, Il-6, TNF-α, and Rankl gene expressions were downregulated in cells cultured on PU and to a higher extent on PA as compared to the corresponding control, whereas Runx, Alpl, Col1a1, and Bglap gene expressions were upregulated vice versa.Conclusion: Our data clearly showed that pectin RG-Is nanocoating with high content of galactan (PA) reduces the osteoblastic response to P. gingivalis infection in vitro and may, therefore, reduce a risk of inflammation especially in immunocompromised patients with rheumatoid or periodontal disorders. Keywords: nanocoatings, Rhamnogalacturonan-I, Porphyromonas gingivalis, osteoblasts, inflammationMeresta AFolkert JGaber TMiksch KButtgereit FDetert JPischon NGurzawska KDove Medical PressarticlenanocoatingsRhamnogalacturonan-IPorphyromonas gingivalisosteoblastsinflammation.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 433-445 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanocoatings Rhamnogalacturonan-I Porphyromonas gingivalis osteoblasts inflammation. Medicine (General) R5-920
spellingShingle	nanocoatings Rhamnogalacturonan-I Porphyromonas gingivalis osteoblasts inflammation. Medicine (General) R5-920 Meresta A Folkert J Gaber T Miksch K Buttgereit F Detert J Pischon N Gurzawska K Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection
description	Anna Meresta,1 Justyna Folkert,1 Timo Gaber,2 Korneliusz Miksch,1 Frank Buttgereit,2 Jacqueline Detert,2 Nicole Pischon,3,* Katarzyna Gurzawska3,4,* 1Environmental Biotechnology Department, Faculty of Power and Environmental, Silesian University of Technology, Gliwice, Poland; 2Department of Rheumatology and Clinical Immunology, 3Department of Periodontology, Charité University Medicine, Berlin, Germany; 4Oral Surgery Department, The School of Dentistry, University of Birmingham, Birmingham, UK *These authors contributed equally to this work Background: Bioengineered plant-derived Rhamnogalacturonan-Is (RG-Is) from pectins are potential candidates for surface nanocoating of medical devices. It has recently been reported that RG-I nanocoatings may prevent bacterial infection and improve the biocompatibility of implants. The aim of the study was to evaluate in vitro impact of bioengineered RG-I nanocoatings on osteogenic capacity and proinflammatory cytokine response of murine osteoblasts following Porphyromonas gingivalis infection.Methods: Murine MC3T3-E1 osteoblasts and isolated primary calvarial osteoblasts from C57BL/6J (B6J osteoblasts) mice were infected with P. gingivalis and incubated on tissue culture polystyrene plates with or without nanocoatings of unmodified RG-Is isolated from potato pulps (PU) or dearabinanated RG-Is (PA). To investigate a behavior of infected osteoblasts cultured on RG-Is cell morphology, proliferation, metabolic activity, mineralization and osteogenic and pro-inflammatory gene expression were examined.Results: Following P. gingivalis infection, PA, but not PU, significantly promoted MC3T3-E1 and BJ6 osteoblasts proliferation, metabolic activity, and calcium deposition. Moreover, Il-1b, Il-6, TNF-α, and Rankl gene expressions were downregulated in cells cultured on PU and to a higher extent on PA as compared to the corresponding control, whereas Runx, Alpl, Col1a1, and Bglap gene expressions were upregulated vice versa.Conclusion: Our data clearly showed that pectin RG-Is nanocoating with high content of galactan (PA) reduces the osteoblastic response to P. gingivalis infection in vitro and may, therefore, reduce a risk of inflammation especially in immunocompromised patients with rheumatoid or periodontal disorders. Keywords: nanocoatings, Rhamnogalacturonan-I, Porphyromonas gingivalis, osteoblasts, inflammation
format	article
author	Meresta A Folkert J Gaber T Miksch K Buttgereit F Detert J Pischon N Gurzawska K
author_facet	Meresta A Folkert J Gaber T Miksch K Buttgereit F Detert J Pischon N Gurzawska K
author_sort	Meresta A
title	Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection
title_short	Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection
title_full	Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection
title_fullStr	Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection
title_full_unstemmed	Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection
title_sort	plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following porphyromonas gingivalis infection
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/0a01db450bac42e986cd53bfe42001e8
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Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection

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