Eleutherine palmifolia (L.) Merr. Extract Increases The Crypts and Caspase-3 Expression in Colitis-Associated Colon Cancer Model

It is known that Eleutherine palmifolia (L.) Merr contains flavonoid and polyphenol as bioactive compounds that have the ability as chemopreventive agents. This study aims to examine the effect of Eleutherine palmifolia (L.) Merr extract (EPE) on colon histopathology and the enhancement of caspase-3...

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Autores principales:	Roihatul Mutiah, Wahyi Yucha Firsyaradha, Riza Ambar Sari, Rahmi Annisa, Risma Aprinda Kristanti, Yen Yen Ari Indrawijaya, Tias Pramesti Griana, Anik Listiyana
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2020
Materias:	eleutherine palmifolia extract colitis-associated colon cancer colonic histopathology caspase-3 Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/0a02c9a2ffd44c8d87f31c7f8564ba7d
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spelling	oai:doaj.org-article:0a02c9a2ffd44c8d87f31c7f8564ba7d2021-11-15T06:08:47ZEleutherine palmifolia (L.) Merr. Extract Increases The Crypts and Caspase-3 Expression in Colitis-Associated Colon Cancer Model2338-94272338-948610.22146/ijp.1120https://doaj.org/article/0a02c9a2ffd44c8d87f31c7f8564ba7d2020-12-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/1120https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486It is known that Eleutherine palmifolia (L.) Merr contains flavonoid and polyphenol as bioactive compounds that have the ability as chemopreventive agents. This study aims to examine the effect of Eleutherine palmifolia (L.) Merr extract (EPE) on colon histopathology and the enhancement of caspase-3 expression in BALB/c mice of colitis-associated colon cancer (CAC) model. Thirty Balb/c female mice were used in this study and were divided into six groups. Five mice in one group were normal or negative control (given phosphate-buffered saline), and twenty-five mice were induced intraperitoneally with 10 mg/kg BW AOM (Azoxymethane), and it was followed by the administration of 5% DSS (Dextran Sodium Sulfate) every two weeks for 20 weeks. At the sixth week, one mice in each group was sacrificed for the Fecal Occult Bold Test (FOBT) and serum amyloid α (SAA) test to ensure that CAC was indeed formed. The administration of EPE with varying doses was started from the eighth week and was continued until the 21st week. The length of the colonic crypt was measured through histology appearance using Hematoxylin-eosin (HE) staining while the immunohistochemical method was used to observe apoptotic activity through the enhancement of caspase-3 expression. The results showed that the increase in EPE dosage also increased the crypt colon length compared to the positive control group. The administration of 1.00 mg/20gBW EPE significantly increased cell apoptosis which can be observed through caspase-3 expression compared to the positive control group (p<0.05). Based on these data, the extract of EPE can be developed as phytotherapy for chemopreventive.Roihatul MutiahWahyi Yucha FirsyaradhaRiza Ambar SariRahmi AnnisaRisma Aprinda KristantiYen Yen Ari IndrawijayaTias Pramesti GrianaAnik ListiyanaUniversitas Gadjah Madaarticleeleutherine palmifolia extractcolitis-associated colon cancercolonic histopathologycaspase-3Pharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 257-265 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	eleutherine palmifolia extract colitis-associated colon cancer colonic histopathology caspase-3 Pharmacy and materia medica RS1-441
spellingShingle	eleutherine palmifolia extract colitis-associated colon cancer colonic histopathology caspase-3 Pharmacy and materia medica RS1-441 Roihatul Mutiah Wahyi Yucha Firsyaradha Riza Ambar Sari Rahmi Annisa Risma Aprinda Kristanti Yen Yen Ari Indrawijaya Tias Pramesti Griana Anik Listiyana Eleutherine palmifolia (L.) Merr. Extract Increases The Crypts and Caspase-3 Expression in Colitis-Associated Colon Cancer Model
description	It is known that Eleutherine palmifolia (L.) Merr contains flavonoid and polyphenol as bioactive compounds that have the ability as chemopreventive agents. This study aims to examine the effect of Eleutherine palmifolia (L.) Merr extract (EPE) on colon histopathology and the enhancement of caspase-3 expression in BALB/c mice of colitis-associated colon cancer (CAC) model. Thirty Balb/c female mice were used in this study and were divided into six groups. Five mice in one group were normal or negative control (given phosphate-buffered saline), and twenty-five mice were induced intraperitoneally with 10 mg/kg BW AOM (Azoxymethane), and it was followed by the administration of 5% DSS (Dextran Sodium Sulfate) every two weeks for 20 weeks. At the sixth week, one mice in each group was sacrificed for the Fecal Occult Bold Test (FOBT) and serum amyloid α (SAA) test to ensure that CAC was indeed formed. The administration of EPE with varying doses was started from the eighth week and was continued until the 21st week. The length of the colonic crypt was measured through histology appearance using Hematoxylin-eosin (HE) staining while the immunohistochemical method was used to observe apoptotic activity through the enhancement of caspase-3 expression. The results showed that the increase in EPE dosage also increased the crypt colon length compared to the positive control group. The administration of 1.00 mg/20gBW EPE significantly increased cell apoptosis which can be observed through caspase-3 expression compared to the positive control group (p<0.05). Based on these data, the extract of EPE can be developed as phytotherapy for chemopreventive.
format	article
author	Roihatul Mutiah Wahyi Yucha Firsyaradha Riza Ambar Sari Rahmi Annisa Risma Aprinda Kristanti Yen Yen Ari Indrawijaya Tias Pramesti Griana Anik Listiyana
author_facet	Roihatul Mutiah Wahyi Yucha Firsyaradha Riza Ambar Sari Rahmi Annisa Risma Aprinda Kristanti Yen Yen Ari Indrawijaya Tias Pramesti Griana Anik Listiyana
author_sort	Roihatul Mutiah
title	Eleutherine palmifolia (L.) Merr. Extract Increases The Crypts and Caspase-3 Expression in Colitis-Associated Colon Cancer Model
title_short	Eleutherine palmifolia (L.) Merr. Extract Increases The Crypts and Caspase-3 Expression in Colitis-Associated Colon Cancer Model
title_full	Eleutherine palmifolia (L.) Merr. Extract Increases The Crypts and Caspase-3 Expression in Colitis-Associated Colon Cancer Model
title_fullStr	Eleutherine palmifolia (L.) Merr. Extract Increases The Crypts and Caspase-3 Expression in Colitis-Associated Colon Cancer Model
title_full_unstemmed	Eleutherine palmifolia (L.) Merr. Extract Increases The Crypts and Caspase-3 Expression in Colitis-Associated Colon Cancer Model
title_sort	eleutherine palmifolia (l.) merr. extract increases the crypts and caspase-3 expression in colitis-associated colon cancer model
publisher	Universitas Gadjah Mada
publishDate	2020
url	https://doaj.org/article/0a02c9a2ffd44c8d87f31c7f8564ba7d
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Eleutherine palmifolia (L.) Merr. Extract Increases The Crypts and Caspase-3 Expression in Colitis-Associated Colon Cancer Model

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