Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii

Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome sequencin...

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Autores principales:	Gema Labrador-Herrera, Antonio J. Pérez-Pulido, Rocío Álvarez-Marín, Carlos S. Casimiro-Soriguer, Tania Cebrero-Cangueiro, Jorgelina Morán-Barrio, Jerónimo Pachón, Alejandro M. Viale, María Eugenia Pachón-Ibáñez
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	acinetobacter baumannii bloodstream infections carbapenem-resistance whole-genome sequencing virulence factor caro Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/0a0c8fc09bb84b9b9751a7d6c0ec34af
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spelling	oai:doaj.org-article:0a0c8fc09bb84b9b9751a7d6c0ec34af2021-11-17T14:21:59ZVirulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii2150-55942150-560810.1080/21505594.2020.1855912https://doaj.org/article/0a0c8fc09bb84b9b9751a7d6c0ec34af2020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1855912https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome sequencing-based comparisons of CRAB clinical isolates causing bloodstream infections secondary to ventilator-associated pneumonia from demographics and clinically homogeneous patients, who received optimal treatment but with different clinical outcomes. Thus, the carO gene was interrupted in CRAB isolates from surviving patients, while it was intact in isolates from non-surviving patients, and proteomic/immunoblot techniques corroborated it. When the virulence role of A. baumannii CarO was analyzed in model systems, isogenic ΔcarO mutants and a CRAB clinical isolate with truncated CarO, showed lower ability to adhere and invade A549 cells and in vivo virulence. This unnoticed virulence role for CarO postulate this A. baumannii outer membrane protein as a potential target for new therapies against CRAB infections.Gema Labrador-HerreraAntonio J. Pérez-PulidoRocío Álvarez-MarínCarlos S. Casimiro-SoriguerTania Cebrero-CangueiroJorgelina Morán-BarrioJerónimo PachónAlejandro M. VialeMaría Eugenia Pachón-IbáñezTaylor & Francis Grouparticleacinetobacter baumanniibloodstream infectionscarbapenem-resistancewhole-genome sequencingvirulence factorcaroInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1727-1737 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	acinetobacter baumannii bloodstream infections carbapenem-resistance whole-genome sequencing virulence factor caro Infectious and parasitic diseases RC109-216
spellingShingle	acinetobacter baumannii bloodstream infections carbapenem-resistance whole-genome sequencing virulence factor caro Infectious and parasitic diseases RC109-216 Gema Labrador-Herrera Antonio J. Pérez-Pulido Rocío Álvarez-Marín Carlos S. Casimiro-Soriguer Tania Cebrero-Cangueiro Jorgelina Morán-Barrio Jerónimo Pachón Alejandro M. Viale María Eugenia Pachón-Ibáñez Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
description	Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome sequencing-based comparisons of CRAB clinical isolates causing bloodstream infections secondary to ventilator-associated pneumonia from demographics and clinically homogeneous patients, who received optimal treatment but with different clinical outcomes. Thus, the carO gene was interrupted in CRAB isolates from surviving patients, while it was intact in isolates from non-surviving patients, and proteomic/immunoblot techniques corroborated it. When the virulence role of A. baumannii CarO was analyzed in model systems, isogenic ΔcarO mutants and a CRAB clinical isolate with truncated CarO, showed lower ability to adhere and invade A549 cells and in vivo virulence. This unnoticed virulence role for CarO postulate this A. baumannii outer membrane protein as a potential target for new therapies against CRAB infections.
format	article
author	Gema Labrador-Herrera Antonio J. Pérez-Pulido Rocío Álvarez-Marín Carlos S. Casimiro-Soriguer Tania Cebrero-Cangueiro Jorgelina Morán-Barrio Jerónimo Pachón Alejandro M. Viale María Eugenia Pachón-Ibáñez
author_facet	Gema Labrador-Herrera Antonio J. Pérez-Pulido Rocío Álvarez-Marín Carlos S. Casimiro-Soriguer Tania Cebrero-Cangueiro Jorgelina Morán-Barrio Jerónimo Pachón Alejandro M. Viale María Eugenia Pachón-Ibáñez
author_sort	Gema Labrador-Herrera
title	Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
title_short	Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
title_full	Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
title_fullStr	Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
title_full_unstemmed	Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
title_sort	virulence role of the outer membrane protein caro in carbapenem-resistant acinetobacter baumannii
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/0a0c8fc09bb84b9b9751a7d6c0ec34af
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Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii

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