Nanoemulsion as a carrier to improve the topical anti-inflammatory activity of stem bark extract of Rapanea ferruginea

Juarana Dal Mas,1 Tailyn Zermiani,1 Liliani C Thiesen,1 Joana LM Silveira,2 Kathryn ABS da Silva,1 Márcia M de Souza,1 Angela Malheiros,1 Tania MB Bresolin,1 Ruth M Lucinda-Silva1 1NIQFAR, Graduate Program in Pharmaceutical Sciences, University of Vale do Itajaí, Itaja&iac...

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Autores principales:	Dal Mas J, Zermiani T, Thiesen LC, Silveira JLM, Silva KABS, de Souza MM, Malheiros A, Bresolin TMB, Lucinda-Silva RM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Nanotechnology Nanoemulsion Rapanea ferruginea Anti-inflammatory Phytomedicine. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0a0ca18132e843ecb8bce30b0cdc312f
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spelling	oai:doaj.org-article:0a0ca18132e843ecb8bce30b0cdc312f2021-12-02T05:38:24ZNanoemulsion as a carrier to improve the topical anti-inflammatory activity of stem bark extract of Rapanea ferruginea1178-2013https://doaj.org/article/0a0ca18132e843ecb8bce30b0cdc312f2016-09-01T00:00:00Zhttps://www.dovepress.com/nanoemulsion-as-a-carrier-to-improve-the-topical-anti-inflammatory-act-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Juarana Dal Mas,1 Tailyn Zermiani,1 Liliani C Thiesen,1 Joana LM Silveira,2 Kathryn ABS da Silva,1 Márcia M de Souza,1 Angela Malheiros,1 Tania MB Bresolin,1 Ruth M Lucinda-Silva1 1NIQFAR, Graduate Program in Pharmaceutical Sciences, University of Vale do Itajaí, Itajaí, Santa Catarina, Brazil; 2Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, Paraná, Brazil Abstract: The aim of this study was to develop nanoemulsion containing soft extract of stem bark of Rapanea ferruginea to improve the topical delivery and anti-inflammatory activity. The extract of R. ferruginea stem bark was incorporated into the oily phase of the nanoemulsion by the method of phase inversion at low energy. The developed nanoemulsion had an average droplet size of 47.88±8.20 nm and a polydispersibility index of 0.228. Uniformity of size, spherical shape of droplet, and absence of clusters were confirmed by transmission electronic microscopy. The zeta potential was -34.7±1.15 mV. The nanoemulsion showed a moderate degree of skin irritation in the agarose overlay assay in vitro. The content of the extract markers, myrsinoic acids A and B, was 54.10±0.08 and 53.03 µg/g in the formulation, respectively. The formulation demonstrated pseudoplastic and thixotropic rheological behavior. In vitro release of chemical markers was controlled by diffusion mechanism. An extract-loaded nanoemulsion showed a topical anti-inflammatory activity in a croton oil-induced edema ear model, with a decrease in tumor necrosis factor release and myeloperoxidase activity. The nanoemulsion was 160% more efficient than the conventional cream containing 0.13% of the extract. The nanoemulsion showed suitable properties as a carrier for topical use of R. ferruginea extract and the approach for improving the topical anti-inflammatory activity. Keywords: nanotechnology, nanoemulsion, Rapanea ferruginea, anti-inflammatory, phytomedicineDal Mas JZermiani TThiesen LCSilveira JLMSilva KABSde Souza MMMalheiros ABresolin TMBLucinda-Silva RMDove Medical PressarticleNanotechnologyNanoemulsionRapanea ferrugineaAnti-inflammatoryPhytomedicine.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 4495-4507 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanotechnology Nanoemulsion Rapanea ferruginea Anti-inflammatory Phytomedicine. Medicine (General) R5-920
spellingShingle	Nanotechnology Nanoemulsion Rapanea ferruginea Anti-inflammatory Phytomedicine. Medicine (General) R5-920 Dal Mas J Zermiani T Thiesen LC Silveira JLM Silva KABS de Souza MM Malheiros A Bresolin TMB Lucinda-Silva RM Nanoemulsion as a carrier to improve the topical anti-inflammatory activity of stem bark extract of Rapanea ferruginea
description	Juarana Dal Mas,1 Tailyn Zermiani,1 Liliani C Thiesen,1 Joana LM Silveira,2 Kathryn ABS da Silva,1 Márcia M de Souza,1 Angela Malheiros,1 Tania MB Bresolin,1 Ruth M Lucinda-Silva1 1NIQFAR, Graduate Program in Pharmaceutical Sciences, University of Vale do Itajaí, Itajaí, Santa Catarina, Brazil; 2Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, Paraná, Brazil Abstract: The aim of this study was to develop nanoemulsion containing soft extract of stem bark of Rapanea ferruginea to improve the topical delivery and anti-inflammatory activity. The extract of R. ferruginea stem bark was incorporated into the oily phase of the nanoemulsion by the method of phase inversion at low energy. The developed nanoemulsion had an average droplet size of 47.88±8.20 nm and a polydispersibility index of 0.228. Uniformity of size, spherical shape of droplet, and absence of clusters were confirmed by transmission electronic microscopy. The zeta potential was -34.7±1.15 mV. The nanoemulsion showed a moderate degree of skin irritation in the agarose overlay assay in vitro. The content of the extract markers, myrsinoic acids A and B, was 54.10±0.08 and 53.03 µg/g in the formulation, respectively. The formulation demonstrated pseudoplastic and thixotropic rheological behavior. In vitro release of chemical markers was controlled by diffusion mechanism. An extract-loaded nanoemulsion showed a topical anti-inflammatory activity in a croton oil-induced edema ear model, with a decrease in tumor necrosis factor release and myeloperoxidase activity. The nanoemulsion was 160% more efficient than the conventional cream containing 0.13% of the extract. The nanoemulsion showed suitable properties as a carrier for topical use of R. ferruginea extract and the approach for improving the topical anti-inflammatory activity. Keywords: nanotechnology, nanoemulsion, Rapanea ferruginea, anti-inflammatory, phytomedicine
format	article
author	Dal Mas J Zermiani T Thiesen LC Silveira JLM Silva KABS de Souza MM Malheiros A Bresolin TMB Lucinda-Silva RM
author_facet	Dal Mas J Zermiani T Thiesen LC Silveira JLM Silva KABS de Souza MM Malheiros A Bresolin TMB Lucinda-Silva RM
author_sort	Dal Mas J
title	Nanoemulsion as a carrier to improve the topical anti-inflammatory activity of stem bark extract of Rapanea ferruginea
title_short	Nanoemulsion as a carrier to improve the topical anti-inflammatory activity of stem bark extract of Rapanea ferruginea
title_full	Nanoemulsion as a carrier to improve the topical anti-inflammatory activity of stem bark extract of Rapanea ferruginea
title_fullStr	Nanoemulsion as a carrier to improve the topical anti-inflammatory activity of stem bark extract of Rapanea ferruginea
title_full_unstemmed	Nanoemulsion as a carrier to improve the topical anti-inflammatory activity of stem bark extract of Rapanea ferruginea
title_sort	nanoemulsion as a carrier to improve the topical anti-inflammatory activity of stem bark extract of rapanea ferruginea
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/0a0ca18132e843ecb8bce30b0cdc312f
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Nanoemulsion as a carrier to improve the topical anti-inflammatory activity of stem bark extract of Rapanea ferruginea

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