Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato

Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato

Abstract DNA-free genome editing was used to induce mutations in one or two branching enzyme genes (Sbe) in tetraploid potato to develop starch with an increased amylose ratio and elongated amylopectin chains. By using ribonucleoprotein (RNP) transfection of potato protoplasts, a mutation frequency...

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Autores principales: Xue Zhao, Shishanthi Jayarathna, Helle Turesson, Ann-Sofie Fält, Gustav Nestor, Matías N. González, Niklas Olsson, Mirela Beganovic, Per Hofvander, Roger Andersson, Mariette Andersson
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
Medicine
R
Science
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Acceso en línea:https://doaj.org/article/0a0e1e75316b49b59c1a61b5e0cd52f4
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