Competition triggers plasmid-mediated enhancement of substrate utilisation in Pseudomonas putida.

Competition between species plays a central role in the activity and structure of communities. Stable co-existence of diverse organisms in communities is thought to be fostered by individual tradeoffs and optimization of competitive strategies along resource gradients. Outside the laboratory, microb...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hiren Joshi, Rachna Dave, Vayalam P Venugopalan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0a0e9c7c154a4e2e9fd4d0c738254a4f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0a0e9c7c154a4e2e9fd4d0c738254a4f
record_format	dspace
spelling	oai:doaj.org-article:0a0e9c7c154a4e2e9fd4d0c738254a4f2021-11-25T06:21:52ZCompetition triggers plasmid-mediated enhancement of substrate utilisation in Pseudomonas putida.1932-620310.1371/journal.pone.0006065https://doaj.org/article/0a0e9c7c154a4e2e9fd4d0c738254a4f2009-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19557171/?tool=EBIhttps://doaj.org/toc/1932-6203Competition between species plays a central role in the activity and structure of communities. Stable co-existence of diverse organisms in communities is thought to be fostered by individual tradeoffs and optimization of competitive strategies along resource gradients. Outside the laboratory, microbes exist as multispecies consortia, continuously interacting with one another and the environment. Survival and proliferation of a particular species is governed by its competitive fitness. Therefore, bacteria must be able to continuously sense their immediate environs for presence of competitors and prevailing conditions. Here we present results of our investigations on a novel competition sensing mechanism in the rhizosphere-inhabiting Pseudomonas putida KT2440, harbouring gfpmut3b-modified Kan(R) TOL plasmid. We monitored benzyl alcohol (BA) degradation rate, along with GFP expression profiling in mono species and dual species cultures. Interestingly, enhanced plasmid expression (monitored using GFP expression) and consequent BA degradation were observed in dual species consortia, irrespective of whether the competitor was a BA degrader (Pseudomonas aeruginosa) or a non-degrader (E. coli). Attempts at elucidation of the mechanistic aspects of induction indicated the role of physical interaction, but not of any diffusible compounds emanating from the competitors. This contention is supported by the observation that greater induction took place in presence of increasing number of competitors. Inert microspheres mimicking competitor cell size and concentration did not elicit any significant induction, further suggesting the role of physical cell-cell interaction. Furthermore, it was also established that cell wall compromised competitor had minimal induction capability. We conclude that P. putida harbouring pWW0 experience a competitive stress when grown as dual-species consortium, irrespective of the counterpart being BA degrader or not. The immediate effect of this stress is a marked increase in expression of TOL, leading to rapid utilization of the available carbon source and massive increase in its population density. The plausible mechanisms behind the phenomenon are hypothesised and practical implications are indicated and discussed.Hiren JoshiRachna DaveVayalam P VenugopalanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 6, p e6065 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hiren Joshi Rachna Dave Vayalam P Venugopalan Competition triggers plasmid-mediated enhancement of substrate utilisation in Pseudomonas putida.
description	Competition between species plays a central role in the activity and structure of communities. Stable co-existence of diverse organisms in communities is thought to be fostered by individual tradeoffs and optimization of competitive strategies along resource gradients. Outside the laboratory, microbes exist as multispecies consortia, continuously interacting with one another and the environment. Survival and proliferation of a particular species is governed by its competitive fitness. Therefore, bacteria must be able to continuously sense their immediate environs for presence of competitors and prevailing conditions. Here we present results of our investigations on a novel competition sensing mechanism in the rhizosphere-inhabiting Pseudomonas putida KT2440, harbouring gfpmut3b-modified Kan(R) TOL plasmid. We monitored benzyl alcohol (BA) degradation rate, along with GFP expression profiling in mono species and dual species cultures. Interestingly, enhanced plasmid expression (monitored using GFP expression) and consequent BA degradation were observed in dual species consortia, irrespective of whether the competitor was a BA degrader (Pseudomonas aeruginosa) or a non-degrader (E. coli). Attempts at elucidation of the mechanistic aspects of induction indicated the role of physical interaction, but not of any diffusible compounds emanating from the competitors. This contention is supported by the observation that greater induction took place in presence of increasing number of competitors. Inert microspheres mimicking competitor cell size and concentration did not elicit any significant induction, further suggesting the role of physical cell-cell interaction. Furthermore, it was also established that cell wall compromised competitor had minimal induction capability. We conclude that P. putida harbouring pWW0 experience a competitive stress when grown as dual-species consortium, irrespective of the counterpart being BA degrader or not. The immediate effect of this stress is a marked increase in expression of TOL, leading to rapid utilization of the available carbon source and massive increase in its population density. The plausible mechanisms behind the phenomenon are hypothesised and practical implications are indicated and discussed.
format	article
author	Hiren Joshi Rachna Dave Vayalam P Venugopalan
author_facet	Hiren Joshi Rachna Dave Vayalam P Venugopalan
author_sort	Hiren Joshi
title	Competition triggers plasmid-mediated enhancement of substrate utilisation in Pseudomonas putida.
title_short	Competition triggers plasmid-mediated enhancement of substrate utilisation in Pseudomonas putida.
title_full	Competition triggers plasmid-mediated enhancement of substrate utilisation in Pseudomonas putida.
title_fullStr	Competition triggers plasmid-mediated enhancement of substrate utilisation in Pseudomonas putida.
title_full_unstemmed	Competition triggers plasmid-mediated enhancement of substrate utilisation in Pseudomonas putida.
title_sort	competition triggers plasmid-mediated enhancement of substrate utilisation in pseudomonas putida.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/0a0e9c7c154a4e2e9fd4d0c738254a4f
work_keys_str_mv	AT hirenjoshi competitiontriggersplasmidmediatedenhancementofsubstrateutilisationinpseudomonasputida AT rachnadave competitiontriggersplasmidmediatedenhancementofsubstrateutilisationinpseudomonasputida AT vayalampvenugopalan competitiontriggersplasmidmediatedenhancementofsubstrateutilisationinpseudomonasputida
_version_	1718413784726372352

Competition triggers plasmid-mediated enhancement of substrate utilisation in Pseudomonas putida.

Ejemplares similares