Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge

Abstract Precise localization of nanoparticles within a cell is crucial to the understanding of cell-particle interactions and has broad applications in nanomedicine. Here, we report a proof-of-principle experiment for imaging individual functionalized nanoparticles within a mammalian cell by correl...

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Autores principales:	Marcus Gallagher-Jones, Carlos Sato Baraldi Dias, Alan Pryor, Karim Bouchmella, Lingrong Zhao, Yuan Hung Lo, Mateus Borba Cardoso, David Shapiro, Jose Rodriguez, Jianwei Miao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0a1291bc67e74932926b35d24d394540
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spelling	oai:doaj.org-article:0a1291bc67e74932926b35d24d3945402021-12-02T12:32:33ZCorrelative cellular ptychography with functionalized nanoparticles at the Fe L-edge10.1038/s41598-017-04784-52045-2322https://doaj.org/article/0a1291bc67e74932926b35d24d3945402017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04784-5https://doaj.org/toc/2045-2322Abstract Precise localization of nanoparticles within a cell is crucial to the understanding of cell-particle interactions and has broad applications in nanomedicine. Here, we report a proof-of-principle experiment for imaging individual functionalized nanoparticles within a mammalian cell by correlative microscopy. Using a chemically-fixed HeLa cell labeled with fluorescent core-shell nanoparticles as a model system, we implemented a graphene-oxide layer as a substrate to significantly reduce background scattering. We identified cellular features of interest by fluorescence microscopy, followed by scanning transmission X-ray tomography to localize the particles in 3D, and ptychographic coherent diffractive imaging of the fine features in the region at high resolution. By tuning the X-ray energy to the Fe L-edge, we demonstrated sensitive detection of nanoparticles composed of a 22 nm magnetic Fe3O4 core encased by a 25-nm-thick fluorescent silica (SiO2) shell. These fluorescent core-shell nanoparticles act as landmarks and offer clarity in a cellular context. Our correlative microscopy results confirmed a subset of particles to be fully internalized, and high-contrast ptychographic images showed two oxidation states of individual nanoparticles with a resolution of ~16.5 nm. The ability to precisely localize individual fluorescent nanoparticles within mammalian cells will expand our understanding of the structure/function relationships for functionalized nanoparticles.Marcus Gallagher-JonesCarlos Sato Baraldi DiasAlan PryorKarim BouchmellaLingrong ZhaoYuan Hung LoMateus Borba CardosoDavid ShapiroJose RodriguezJianwei MiaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marcus Gallagher-Jones Carlos Sato Baraldi Dias Alan Pryor Karim Bouchmella Lingrong Zhao Yuan Hung Lo Mateus Borba Cardoso David Shapiro Jose Rodriguez Jianwei Miao Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge
description	Abstract Precise localization of nanoparticles within a cell is crucial to the understanding of cell-particle interactions and has broad applications in nanomedicine. Here, we report a proof-of-principle experiment for imaging individual functionalized nanoparticles within a mammalian cell by correlative microscopy. Using a chemically-fixed HeLa cell labeled with fluorescent core-shell nanoparticles as a model system, we implemented a graphene-oxide layer as a substrate to significantly reduce background scattering. We identified cellular features of interest by fluorescence microscopy, followed by scanning transmission X-ray tomography to localize the particles in 3D, and ptychographic coherent diffractive imaging of the fine features in the region at high resolution. By tuning the X-ray energy to the Fe L-edge, we demonstrated sensitive detection of nanoparticles composed of a 22 nm magnetic Fe3O4 core encased by a 25-nm-thick fluorescent silica (SiO2) shell. These fluorescent core-shell nanoparticles act as landmarks and offer clarity in a cellular context. Our correlative microscopy results confirmed a subset of particles to be fully internalized, and high-contrast ptychographic images showed two oxidation states of individual nanoparticles with a resolution of ~16.5 nm. The ability to precisely localize individual fluorescent nanoparticles within mammalian cells will expand our understanding of the structure/function relationships for functionalized nanoparticles.
format	article
author	Marcus Gallagher-Jones Carlos Sato Baraldi Dias Alan Pryor Karim Bouchmella Lingrong Zhao Yuan Hung Lo Mateus Borba Cardoso David Shapiro Jose Rodriguez Jianwei Miao
author_facet	Marcus Gallagher-Jones Carlos Sato Baraldi Dias Alan Pryor Karim Bouchmella Lingrong Zhao Yuan Hung Lo Mateus Borba Cardoso David Shapiro Jose Rodriguez Jianwei Miao
author_sort	Marcus Gallagher-Jones
title	Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge
title_short	Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge
title_full	Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge
title_fullStr	Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge
title_full_unstemmed	Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge
title_sort	correlative cellular ptychography with functionalized nanoparticles at the fe l-edge
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0a1291bc67e74932926b35d24d394540
work_keys_str_mv	AT marcusgallagherjones correlativecellularptychographywithfunctionalizednanoparticlesatthefeledge AT carlossatobaraldidias correlativecellularptychographywithfunctionalizednanoparticlesatthefeledge AT alanpryor correlativecellularptychographywithfunctionalizednanoparticlesatthefeledge AT karimbouchmella correlativecellularptychographywithfunctionalizednanoparticlesatthefeledge AT lingrongzhao correlativecellularptychographywithfunctionalizednanoparticlesatthefeledge AT yuanhunglo correlativecellularptychographywithfunctionalizednanoparticlesatthefeledge AT mateusborbacardoso correlativecellularptychographywithfunctionalizednanoparticlesatthefeledge AT davidshapiro correlativecellularptychographywithfunctionalizednanoparticlesatthefeledge AT joserodriguez correlativecellularptychographywithfunctionalizednanoparticlesatthefeledge AT jianweimiao correlativecellularptychographywithfunctionalizednanoparticlesatthefeledge
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Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge

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