Draft genome sequencing of giardia intestinalis assemblage B isolate GS: is human giardiasis caused by two different species?

Giardia intestinalis is a major cause of diarrheal disease worldwide and two major Giardia genotypes, assemblages A and B, infect humans. The genome of assemblage A parasite WB was recently sequenced, and the structurally compact 11.7 Mbp genome contains simplified basic cellular machineries and met...

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Autores principales:	Oscar Franzén, Jon Jerlström-Hultqvist, Elsie Castro, Ellen Sherwood, Johan Ankarklev, David S Reiner, Daniel Palm, Jan O Andersson, Björn Andersson, Staffan G Svärd
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0a1c121847c148da90041f5828502046
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spelling	oai:doaj.org-article:0a1c121847c148da90041f58285020462021-11-25T05:47:42ZDraft genome sequencing of giardia intestinalis assemblage B isolate GS: is human giardiasis caused by two different species?1553-73661553-737410.1371/journal.ppat.1000560https://doaj.org/article/0a1c121847c148da90041f58285020462009-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19696920/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Giardia intestinalis is a major cause of diarrheal disease worldwide and two major Giardia genotypes, assemblages A and B, infect humans. The genome of assemblage A parasite WB was recently sequenced, and the structurally compact 11.7 Mbp genome contains simplified basic cellular machineries and metabolism. We here performed 454 sequencing to 16x coverage of the assemblage B isolate GS, the only Giardia isolate successfully used to experimentally infect animals and humans. The two genomes show 77% nucleotide and 78% amino-acid identity in protein coding regions. Comparative analysis identified 28 unique GS and 3 unique WB protein coding genes, and the variable surface protein (VSP) repertoires of the two isolates are completely different. The promoters of several enzymes involved in the synthesis of the cyst-wall lack binding sites for encystation-specific transcription factors in GS. Several synteny-breaks were detected and verified. The tetraploid GS genome shows higher levels of overall allelic sequence polymorphism (0.5 versus <0.01% in WB). The genomic differences between WB and GS may explain some of the observed biological and clinical differences between the two isolates, and it suggests that assemblage A and B Giardia can be two different species.Oscar FranzénJon Jerlström-HultqvistElsie CastroEllen SherwoodJohan AnkarklevDavid S ReinerDaniel PalmJan O AnderssonBjörn AnderssonStaffan G SvärdPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 5, Iss 8, p e1000560 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Oscar Franzén Jon Jerlström-Hultqvist Elsie Castro Ellen Sherwood Johan Ankarklev David S Reiner Daniel Palm Jan O Andersson Björn Andersson Staffan G Svärd Draft genome sequencing of giardia intestinalis assemblage B isolate GS: is human giardiasis caused by two different species?
description	Giardia intestinalis is a major cause of diarrheal disease worldwide and two major Giardia genotypes, assemblages A and B, infect humans. The genome of assemblage A parasite WB was recently sequenced, and the structurally compact 11.7 Mbp genome contains simplified basic cellular machineries and metabolism. We here performed 454 sequencing to 16x coverage of the assemblage B isolate GS, the only Giardia isolate successfully used to experimentally infect animals and humans. The two genomes show 77% nucleotide and 78% amino-acid identity in protein coding regions. Comparative analysis identified 28 unique GS and 3 unique WB protein coding genes, and the variable surface protein (VSP) repertoires of the two isolates are completely different. The promoters of several enzymes involved in the synthesis of the cyst-wall lack binding sites for encystation-specific transcription factors in GS. Several synteny-breaks were detected and verified. The tetraploid GS genome shows higher levels of overall allelic sequence polymorphism (0.5 versus <0.01% in WB). The genomic differences between WB and GS may explain some of the observed biological and clinical differences between the two isolates, and it suggests that assemblage A and B Giardia can be two different species.
format	article
author	Oscar Franzén Jon Jerlström-Hultqvist Elsie Castro Ellen Sherwood Johan Ankarklev David S Reiner Daniel Palm Jan O Andersson Björn Andersson Staffan G Svärd
author_facet	Oscar Franzén Jon Jerlström-Hultqvist Elsie Castro Ellen Sherwood Johan Ankarklev David S Reiner Daniel Palm Jan O Andersson Björn Andersson Staffan G Svärd
author_sort	Oscar Franzén
title	Draft genome sequencing of giardia intestinalis assemblage B isolate GS: is human giardiasis caused by two different species?
title_short	Draft genome sequencing of giardia intestinalis assemblage B isolate GS: is human giardiasis caused by two different species?
title_full	Draft genome sequencing of giardia intestinalis assemblage B isolate GS: is human giardiasis caused by two different species?
title_fullStr	Draft genome sequencing of giardia intestinalis assemblage B isolate GS: is human giardiasis caused by two different species?
title_full_unstemmed	Draft genome sequencing of giardia intestinalis assemblage B isolate GS: is human giardiasis caused by two different species?
title_sort	draft genome sequencing of giardia intestinalis assemblage b isolate gs: is human giardiasis caused by two different species?
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/0a1c121847c148da90041f5828502046
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Draft genome sequencing of giardia intestinalis assemblage B isolate GS: is human giardiasis caused by two different species?

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