Pilot Study of 64Cu(I) for PET Imaging of Melanoma

Abstract At present, 64Cu(II) labeled tracers including 64CuCl2 have been widely applied in the research of molecular imaging and therapy. Human copper transporter 1 (hCTR1) is the major high affinity copper influx transporter in mammalian cells, and specially responsible for the transportation of C...

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Autores principales:	Lei Jiang, Yingfeng Tu, Xiang Hu, Ande Bao, Hao Chen, Xiaowei Ma, Tim Doyle, Hongcheng Shi, Zhen Cheng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0a1e3346b30e4804ba2eb5c78777a902
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spelling	oai:doaj.org-article:0a1e3346b30e4804ba2eb5c78777a9022021-12-02T11:52:30ZPilot Study of 64Cu(I) for PET Imaging of Melanoma10.1038/s41598-017-02691-32045-2322https://doaj.org/article/0a1e3346b30e4804ba2eb5c78777a9022017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02691-3https://doaj.org/toc/2045-2322Abstract At present, 64Cu(II) labeled tracers including 64CuCl2 have been widely applied in the research of molecular imaging and therapy. Human copper transporter 1 (hCTR1) is the major high affinity copper influx transporter in mammalian cells, and specially responsible for the transportation of Cu(I) not Cu(II). Thus, we investigated the feasible application of 64Cu(I) for PET imaging. 64Cu(II) was reduced to 64Cu(I) with the existence of sodium L-ascorbate, DL-Dithiothreitol or cysteine. Cell uptake and efflux assay was investigated using B16F10 and A375 cell lines, respectively. Small animal PET and biodistribution studies were performed in both B16F10 and A375 tumor-bearing mice. Compared with 64Cu(II), 64Cu(I) exhibited higher cellular uptake by melanoma, which testified CTR1 specially influx of Cu(I). However, due to oxidation reaction in vivo, no significant difference between 64Cu(I) and 64Cu(II) was observed through PET images and biodistribution. Additionally, radiation absorbed doses for major tissues of human were calculated based on the mouse biodistribution. Radiodosimetry calculations for 64/67Cu(I) and 64/67Cu(II) were similar, which suggested that although melanoma were with high radiation absorbed doses, high radioactivity accumulation by liver and kidney should be noticed for the further application. Thus, 64Cu(I) should be further studied to evaluate it as a PET imaging radiotracer.Lei JiangYingfeng TuXiang HuAnde BaoHao ChenXiaowei MaTim DoyleHongcheng ShiZhen ChengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lei Jiang Yingfeng Tu Xiang Hu Ande Bao Hao Chen Xiaowei Ma Tim Doyle Hongcheng Shi Zhen Cheng Pilot Study of 64Cu(I) for PET Imaging of Melanoma
description	Abstract At present, 64Cu(II) labeled tracers including 64CuCl2 have been widely applied in the research of molecular imaging and therapy. Human copper transporter 1 (hCTR1) is the major high affinity copper influx transporter in mammalian cells, and specially responsible for the transportation of Cu(I) not Cu(II). Thus, we investigated the feasible application of 64Cu(I) for PET imaging. 64Cu(II) was reduced to 64Cu(I) with the existence of sodium L-ascorbate, DL-Dithiothreitol or cysteine. Cell uptake and efflux assay was investigated using B16F10 and A375 cell lines, respectively. Small animal PET and biodistribution studies were performed in both B16F10 and A375 tumor-bearing mice. Compared with 64Cu(II), 64Cu(I) exhibited higher cellular uptake by melanoma, which testified CTR1 specially influx of Cu(I). However, due to oxidation reaction in vivo, no significant difference between 64Cu(I) and 64Cu(II) was observed through PET images and biodistribution. Additionally, radiation absorbed doses for major tissues of human were calculated based on the mouse biodistribution. Radiodosimetry calculations for 64/67Cu(I) and 64/67Cu(II) were similar, which suggested that although melanoma were with high radiation absorbed doses, high radioactivity accumulation by liver and kidney should be noticed for the further application. Thus, 64Cu(I) should be further studied to evaluate it as a PET imaging radiotracer.
format	article
author	Lei Jiang Yingfeng Tu Xiang Hu Ande Bao Hao Chen Xiaowei Ma Tim Doyle Hongcheng Shi Zhen Cheng
author_facet	Lei Jiang Yingfeng Tu Xiang Hu Ande Bao Hao Chen Xiaowei Ma Tim Doyle Hongcheng Shi Zhen Cheng
author_sort	Lei Jiang
title	Pilot Study of 64Cu(I) for PET Imaging of Melanoma
title_short	Pilot Study of 64Cu(I) for PET Imaging of Melanoma
title_full	Pilot Study of 64Cu(I) for PET Imaging of Melanoma
title_fullStr	Pilot Study of 64Cu(I) for PET Imaging of Melanoma
title_full_unstemmed	Pilot Study of 64Cu(I) for PET Imaging of Melanoma
title_sort	pilot study of 64cu(i) for pet imaging of melanoma
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0a1e3346b30e4804ba2eb5c78777a902
work_keys_str_mv	AT leijiang pilotstudyof64cuiforpetimagingofmelanoma AT yingfengtu pilotstudyof64cuiforpetimagingofmelanoma AT xianghu pilotstudyof64cuiforpetimagingofmelanoma AT andebao pilotstudyof64cuiforpetimagingofmelanoma AT haochen pilotstudyof64cuiforpetimagingofmelanoma AT xiaoweima pilotstudyof64cuiforpetimagingofmelanoma AT timdoyle pilotstudyof64cuiforpetimagingofmelanoma AT hongchengshi pilotstudyof64cuiforpetimagingofmelanoma AT zhencheng pilotstudyof64cuiforpetimagingofmelanoma
_version_	1718395028525547520

Pilot Study of 64Cu(I) for PET Imaging of Melanoma

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