Unexpected localization of AQP3 and AQP4 induced by migration of primary cultured IMCD cells

Abstract Aquaporin-2–4 (AQP) are expressed in the principal cells of the renal collecting duct (CD). Beside their role in water transport across membranes, several studies showed that AQPs can influence the migration of cells. It is unknown whether this also applies for renal CD cells. Another fact...

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Autores principales:	Ralph Rose, Björn Kemper, Albrecht Schwab, Eberhard Schlatter, Bayram Edemir
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0a225194b1554530bb1d639f14dd5a8e
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spelling	oai:doaj.org-article:0a225194b1554530bb1d639f14dd5a8e2021-12-02T17:34:40ZUnexpected localization of AQP3 and AQP4 induced by migration of primary cultured IMCD cells10.1038/s41598-021-91369-y2045-2322https://doaj.org/article/0a225194b1554530bb1d639f14dd5a8e2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91369-yhttps://doaj.org/toc/2045-2322Abstract Aquaporin-2–4 (AQP) are expressed in the principal cells of the renal collecting duct (CD). Beside their role in water transport across membranes, several studies showed that AQPs can influence the migration of cells. It is unknown whether this also applies for renal CD cells. Another fact is that the expression of these AQPs is highly modulated by the external osmolality. Here we analyzed the localization of AQP2–4 in primary cultured renal inner medullary CD (IMCD) cells and how osmolality influences the migration behavior of these cells. The primary IMCD cells showed a collective migration behavior and there were no differences in the migration speed between cells cultivated either at 300 or 600 mosmol/kg. Acute increase from 300 to 600 mosmol/kg led to a marked reduction and vice versa an acute decrease from 600 to 300 mosmol/kg to a marked increase in migration speed. Interestingly, none of the analyzed AQPs were localized at the leading edge. While AQP3 disappeared within the first 2–3 rows of cells, AQP4 was enriched at the rear end. Further analysis indicated that migration induced lysosomal degradation of AQP3. This could be prevented by activation of the protein kinase A, inducing localization of AQP3 and AQP2 at the leading edge and increasing the migration speed.Ralph RoseBjörn KemperAlbrecht SchwabEberhard SchlatterBayram EdemirNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ralph Rose Björn Kemper Albrecht Schwab Eberhard Schlatter Bayram Edemir Unexpected localization of AQP3 and AQP4 induced by migration of primary cultured IMCD cells
description	Abstract Aquaporin-2–4 (AQP) are expressed in the principal cells of the renal collecting duct (CD). Beside their role in water transport across membranes, several studies showed that AQPs can influence the migration of cells. It is unknown whether this also applies for renal CD cells. Another fact is that the expression of these AQPs is highly modulated by the external osmolality. Here we analyzed the localization of AQP2–4 in primary cultured renal inner medullary CD (IMCD) cells and how osmolality influences the migration behavior of these cells. The primary IMCD cells showed a collective migration behavior and there were no differences in the migration speed between cells cultivated either at 300 or 600 mosmol/kg. Acute increase from 300 to 600 mosmol/kg led to a marked reduction and vice versa an acute decrease from 600 to 300 mosmol/kg to a marked increase in migration speed. Interestingly, none of the analyzed AQPs were localized at the leading edge. While AQP3 disappeared within the first 2–3 rows of cells, AQP4 was enriched at the rear end. Further analysis indicated that migration induced lysosomal degradation of AQP3. This could be prevented by activation of the protein kinase A, inducing localization of AQP3 and AQP2 at the leading edge and increasing the migration speed.
format	article
author	Ralph Rose Björn Kemper Albrecht Schwab Eberhard Schlatter Bayram Edemir
author_facet	Ralph Rose Björn Kemper Albrecht Schwab Eberhard Schlatter Bayram Edemir
author_sort	Ralph Rose
title	Unexpected localization of AQP3 and AQP4 induced by migration of primary cultured IMCD cells
title_short	Unexpected localization of AQP3 and AQP4 induced by migration of primary cultured IMCD cells
title_full	Unexpected localization of AQP3 and AQP4 induced by migration of primary cultured IMCD cells
title_fullStr	Unexpected localization of AQP3 and AQP4 induced by migration of primary cultured IMCD cells
title_full_unstemmed	Unexpected localization of AQP3 and AQP4 induced by migration of primary cultured IMCD cells
title_sort	unexpected localization of aqp3 and aqp4 induced by migration of primary cultured imcd cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0a225194b1554530bb1d639f14dd5a8e
work_keys_str_mv	AT ralphrose unexpectedlocalizationofaqp3andaqp4inducedbymigrationofprimaryculturedimcdcells AT bjornkemper unexpectedlocalizationofaqp3andaqp4inducedbymigrationofprimaryculturedimcdcells AT albrechtschwab unexpectedlocalizationofaqp3andaqp4inducedbymigrationofprimaryculturedimcdcells AT eberhardschlatter unexpectedlocalizationofaqp3andaqp4inducedbymigrationofprimaryculturedimcdcells AT bayramedemir unexpectedlocalizationofaqp3andaqp4inducedbymigrationofprimaryculturedimcdcells
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Unexpected localization of AQP3 and AQP4 induced by migration of primary cultured IMCD cells

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