The Use of Real-Time Polymerase Chain Reaction Combined with Specific-Species Primer for Analysis of Dog Meat DNA in Meatball

The presence of dog meat is a crucial issue because dog meat is non-halal meat for Muslims. The objective of this study was to design and validate species-specific primer for the identification of dog meat DNA in meatball using real-time polymerase chain reaction (real-time PCR). The specific primer...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Abdul Rohman, Wiranti Sri Rahayu, Sudjadi Sudjadi, Sudibyo Martono
Formato:	article
Lenguaje:	EN
Publicado:	Department of Chemistry, Universitas Gadjah Mada 2020
Materias:	dog meat meatball real-time polymerase chain reaction halal authentication Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/0a2481d58d424e2183e135619993209b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0a2481d58d424e2183e135619993209b
record_format	dspace
spelling	oai:doaj.org-article:0a2481d58d424e2183e135619993209b2021-12-02T14:02:46ZThe Use of Real-Time Polymerase Chain Reaction Combined with Specific-Species Primer for Analysis of Dog Meat DNA in Meatball1411-94202460-157810.22146/ijc.48930https://doaj.org/article/0a2481d58d424e2183e135619993209b2020-09-01T00:00:00Zhttps://jurnal.ugm.ac.id/ijc/article/view/48930https://doaj.org/toc/1411-9420https://doaj.org/toc/2460-1578The presence of dog meat is a crucial issue because dog meat is non-halal meat for Muslims. The objective of this study was to design and validate species-specific primer for the identification of dog meat DNA in meatball using real-time polymerase chain reaction (real-time PCR). The specific primer targeting mitochondrial cytochrome c oxidase subunit 1 (CO1) was validated. The specific primers used were designed using Integrated DNA Technologies (IDT) software and subjected to NCBI BLAST procedure. The candidate primers were tested for specificity study using several DNAs from fresh meat of pork, chicken, beef, lamb, and rat. The method was also validated by determining several parameters of linearity, sensitivity, precision, and efficiency. The results showed that primer could amplify specifically DNA target at an optimized annealing temperature of 56.6 °C. The limit of detection (LoD) obtained was 5 ng DNA, corresponding to 2.5% of dog meat in a meatball. The repeatability evaluation, expressed with relative standard deviation (RSD), and efficiency value was in the acceptable range (RSD < 25% and efficiency (90–105%). This method was successfully used for the analysis of marketed samples. Real-time PCR can be used as a standard method in halal authentication analysis through DNA analysis.Abdul RohmanWiranti Sri RahayuSudjadi SudjadiSudibyo MartonoDepartment of Chemistry, Universitas Gadjah Madaarticledog meatmeatballreal-time polymerase chain reactionhalal authenticationChemistryQD1-999ENIndonesian Journal of Chemistry, Vol 21, Iss 1, Pp 225-233 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	dog meat meatball real-time polymerase chain reaction halal authentication Chemistry QD1-999
spellingShingle	dog meat meatball real-time polymerase chain reaction halal authentication Chemistry QD1-999 Abdul Rohman Wiranti Sri Rahayu Sudjadi Sudjadi Sudibyo Martono The Use of Real-Time Polymerase Chain Reaction Combined with Specific-Species Primer for Analysis of Dog Meat DNA in Meatball
description	The presence of dog meat is a crucial issue because dog meat is non-halal meat for Muslims. The objective of this study was to design and validate species-specific primer for the identification of dog meat DNA in meatball using real-time polymerase chain reaction (real-time PCR). The specific primer targeting mitochondrial cytochrome c oxidase subunit 1 (CO1) was validated. The specific primers used were designed using Integrated DNA Technologies (IDT) software and subjected to NCBI BLAST procedure. The candidate primers were tested for specificity study using several DNAs from fresh meat of pork, chicken, beef, lamb, and rat. The method was also validated by determining several parameters of linearity, sensitivity, precision, and efficiency. The results showed that primer could amplify specifically DNA target at an optimized annealing temperature of 56.6 °C. The limit of detection (LoD) obtained was 5 ng DNA, corresponding to 2.5% of dog meat in a meatball. The repeatability evaluation, expressed with relative standard deviation (RSD), and efficiency value was in the acceptable range (RSD < 25% and efficiency (90–105%). This method was successfully used for the analysis of marketed samples. Real-time PCR can be used as a standard method in halal authentication analysis through DNA analysis.
format	article
author	Abdul Rohman Wiranti Sri Rahayu Sudjadi Sudjadi Sudibyo Martono
author_facet	Abdul Rohman Wiranti Sri Rahayu Sudjadi Sudjadi Sudibyo Martono
author_sort	Abdul Rohman
title	The Use of Real-Time Polymerase Chain Reaction Combined with Specific-Species Primer for Analysis of Dog Meat DNA in Meatball
title_short	The Use of Real-Time Polymerase Chain Reaction Combined with Specific-Species Primer for Analysis of Dog Meat DNA in Meatball
title_full	The Use of Real-Time Polymerase Chain Reaction Combined with Specific-Species Primer for Analysis of Dog Meat DNA in Meatball
title_fullStr	The Use of Real-Time Polymerase Chain Reaction Combined with Specific-Species Primer for Analysis of Dog Meat DNA in Meatball
title_full_unstemmed	The Use of Real-Time Polymerase Chain Reaction Combined with Specific-Species Primer for Analysis of Dog Meat DNA in Meatball
title_sort	use of real-time polymerase chain reaction combined with specific-species primer for analysis of dog meat dna in meatball
publisher	Department of Chemistry, Universitas Gadjah Mada
publishDate	2020
url	https://doaj.org/article/0a2481d58d424e2183e135619993209b
work_keys_str_mv	AT abdulrohman theuseofrealtimepolymerasechainreactioncombinedwithspecificspeciesprimerforanalysisofdogmeatdnainmeatball AT wirantisrirahayu theuseofrealtimepolymerasechainreactioncombinedwithspecificspeciesprimerforanalysisofdogmeatdnainmeatball AT sudjadisudjadi theuseofrealtimepolymerasechainreactioncombinedwithspecificspeciesprimerforanalysisofdogmeatdnainmeatball AT sudibyomartono theuseofrealtimepolymerasechainreactioncombinedwithspecificspeciesprimerforanalysisofdogmeatdnainmeatball AT abdulrohman useofrealtimepolymerasechainreactioncombinedwithspecificspeciesprimerforanalysisofdogmeatdnainmeatball AT wirantisrirahayu useofrealtimepolymerasechainreactioncombinedwithspecificspeciesprimerforanalysisofdogmeatdnainmeatball AT sudjadisudjadi useofrealtimepolymerasechainreactioncombinedwithspecificspeciesprimerforanalysisofdogmeatdnainmeatball AT sudibyomartono useofrealtimepolymerasechainreactioncombinedwithspecificspeciesprimerforanalysisofdogmeatdnainmeatball
_version_	1718392064120455168

The Use of Real-Time Polymerase Chain Reaction Combined with Specific-Species Primer for Analysis of Dog Meat DNA in Meatball

Ejemplares similares