Inhibition of the differentiation of monocyte-derived dendritic cells by human gingival fibroblasts.

We investigated whether gingival fibroblasts (GFs) can modulate the differentiation and/or maturation of monocyte-derived dendritic cells (DCs) and analyzed soluble factors that may be involved in this immune modulation. Experiments were performed using human monocytes in co-culture with human GFs i...

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Autores principales:	Sylvie Séguier, Eric Tartour, Coralie Guérin, Ludovic Couty, Mathilde Lemitre, Laetitia Lallement, Marysette Folliguet, Samah El Naderi, Magali Terme, Cécile Badoual, Antoine Lafont, Bernard Coulomb
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0a38a90abeeb4fbdb66a297b497499e8
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spelling	oai:doaj.org-article:0a38a90abeeb4fbdb66a297b497499e82021-11-18T09:01:30ZInhibition of the differentiation of monocyte-derived dendritic cells by human gingival fibroblasts.1932-620310.1371/journal.pone.0070937https://doaj.org/article/0a38a90abeeb4fbdb66a297b497499e82013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23936476/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We investigated whether gingival fibroblasts (GFs) can modulate the differentiation and/or maturation of monocyte-derived dendritic cells (DCs) and analyzed soluble factors that may be involved in this immune modulation. Experiments were performed using human monocytes in co-culture with human GFs in Transwell® chambers or using monocyte cultures treated with conditioned media (CM) from GFs of four donors. The four CM and supernatants from cell culture were assayed by ELISA for cytokines involved in the differentiation of dendritic cells, such as IL-6, VEGF, TGFβ1, IL-13 and IL-10. The maturation of monocyte-derived DCs induced by LPS in presence of CM was also studied. Cell surface phenotype markers were analyzed by flow cytometry. In co-cultures, GFs inhibited the differentiation of monocyte-derived DCs and the strength of this blockade correlated with the GF/monocyte ratio. Conditioned media from GFs showed similar effects, suggesting the involvement of soluble factors produced by GFs. This inhibition was associated with a lower stimulatory activity in MLR of DCs generated with GFs or its CM. Neutralizing antibodies against IL-6 and VEGF significantly (P<0.05) inhibited the inhibitory effect of CM on the differentiation of monocytes-derived DCs and in a dose dependent manner. Our data suggest that IL-6 is the main factor responsible for the inhibition of DCs differentiation mediated by GFs but that VEGF is also involved and constitutes an additional mechanism.Sylvie SéguierEric TartourCoralie GuérinLudovic CoutyMathilde LemitreLaetitia LallementMarysette FolliguetSamah El NaderiMagali TermeCécile BadoualAntoine LafontBernard CoulombPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e70937 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sylvie Séguier Eric Tartour Coralie Guérin Ludovic Couty Mathilde Lemitre Laetitia Lallement Marysette Folliguet Samah El Naderi Magali Terme Cécile Badoual Antoine Lafont Bernard Coulomb Inhibition of the differentiation of monocyte-derived dendritic cells by human gingival fibroblasts.
description	We investigated whether gingival fibroblasts (GFs) can modulate the differentiation and/or maturation of monocyte-derived dendritic cells (DCs) and analyzed soluble factors that may be involved in this immune modulation. Experiments were performed using human monocytes in co-culture with human GFs in Transwell® chambers or using monocyte cultures treated with conditioned media (CM) from GFs of four donors. The four CM and supernatants from cell culture were assayed by ELISA for cytokines involved in the differentiation of dendritic cells, such as IL-6, VEGF, TGFβ1, IL-13 and IL-10. The maturation of monocyte-derived DCs induced by LPS in presence of CM was also studied. Cell surface phenotype markers were analyzed by flow cytometry. In co-cultures, GFs inhibited the differentiation of monocyte-derived DCs and the strength of this blockade correlated with the GF/monocyte ratio. Conditioned media from GFs showed similar effects, suggesting the involvement of soluble factors produced by GFs. This inhibition was associated with a lower stimulatory activity in MLR of DCs generated with GFs or its CM. Neutralizing antibodies against IL-6 and VEGF significantly (P<0.05) inhibited the inhibitory effect of CM on the differentiation of monocytes-derived DCs and in a dose dependent manner. Our data suggest that IL-6 is the main factor responsible for the inhibition of DCs differentiation mediated by GFs but that VEGF is also involved and constitutes an additional mechanism.
format	article
author	Sylvie Séguier Eric Tartour Coralie Guérin Ludovic Couty Mathilde Lemitre Laetitia Lallement Marysette Folliguet Samah El Naderi Magali Terme Cécile Badoual Antoine Lafont Bernard Coulomb
author_facet	Sylvie Séguier Eric Tartour Coralie Guérin Ludovic Couty Mathilde Lemitre Laetitia Lallement Marysette Folliguet Samah El Naderi Magali Terme Cécile Badoual Antoine Lafont Bernard Coulomb
author_sort	Sylvie Séguier
title	Inhibition of the differentiation of monocyte-derived dendritic cells by human gingival fibroblasts.
title_short	Inhibition of the differentiation of monocyte-derived dendritic cells by human gingival fibroblasts.
title_full	Inhibition of the differentiation of monocyte-derived dendritic cells by human gingival fibroblasts.
title_fullStr	Inhibition of the differentiation of monocyte-derived dendritic cells by human gingival fibroblasts.
title_full_unstemmed	Inhibition of the differentiation of monocyte-derived dendritic cells by human gingival fibroblasts.
title_sort	inhibition of the differentiation of monocyte-derived dendritic cells by human gingival fibroblasts.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/0a38a90abeeb4fbdb66a297b497499e8
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Inhibition of the differentiation of monocyte-derived dendritic cells by human gingival fibroblasts.

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