Outer membrane vesicles secreted by pathogenic and nonpathogenic Bacteroides fragilis represent different metabolic activities

Abstract Numerous studies are devoted to the intestinal microbiota and intercellular communication maintaining homeostasis. In this regard, vesicles secreted by bacteria represent one of the most popular topics for research. For example, the outer membrane vesicles (OMVs) of Bacteroides fragilis pla...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Natalya B. Zakharzhevskaya, Anna A. Vanyushkina, Ilya A. Altukhov, Aleksey L. Shavarda, Ivan O. Butenko, Daria V. Rakitina, Anastasia S. Nikitina, Aleksandr I. Manolov, Alina N. Egorova, Eugene E. Kulikov, Innokentii E. Vishnyakov, Gleb Y. Fisunov, Vadim M. Govorun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0a3a1a392b52497794ce3934fcb2bbae
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0a3a1a392b52497794ce3934fcb2bbae
record_format	dspace
spelling	oai:doaj.org-article:0a3a1a392b52497794ce3934fcb2bbae2021-12-02T12:30:25ZOuter membrane vesicles secreted by pathogenic and nonpathogenic Bacteroides fragilis represent different metabolic activities10.1038/s41598-017-05264-62045-2322https://doaj.org/article/0a3a1a392b52497794ce3934fcb2bbae2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05264-6https://doaj.org/toc/2045-2322Abstract Numerous studies are devoted to the intestinal microbiota and intercellular communication maintaining homeostasis. In this regard, vesicles secreted by bacteria represent one of the most popular topics for research. For example, the outer membrane vesicles (OMVs) of Bacteroides fragilis play an important nutritional role with respect to other microorganisms and promote anti-inflammatory effects on immune cells. However, toxigenic B. fragilis (ETBF) contributes to bowel disease, even causing colon cancer. If nontoxigenic B. fragilis (NTBF) vesicles exert a beneficial effect on the intestine, it is likely that ETBF vesicles can be utilized for potential pathogenic implementation. To confirm this possibility, we performed comparative proteomic HPLC-MS/MS analysis of vesicles isolated from ETBF and NTBF. Furthermore, we performed, for the first time, HPLC-MS/MS and GS-MS comparative metabolomic analysis for the vesicles isolated from both strains with subsequent reconstruction of the vesicle metabolic pathways. We utilized fluxomic experiments to validate the reconstructed biochemical reaction activities and finally observed considerable difference in the vesicle proteome and metabolome profiles. Compared with NTBF OMVs, metabolic activity of ETBF OMVs provides their similarity to micro reactors that are likely to be used for long-term persistence and implementing pathogenic potential in the host.Natalya B. ZakharzhevskayaAnna A. VanyushkinaIlya A. AltukhovAleksey L. ShavardaIvan O. ButenkoDaria V. RakitinaAnastasia S. NikitinaAleksandr I. ManolovAlina N. EgorovaEugene E. KulikovInnokentii E. VishnyakovGleb Y. FisunovVadim M. GovorunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Natalya B. Zakharzhevskaya Anna A. Vanyushkina Ilya A. Altukhov Aleksey L. Shavarda Ivan O. Butenko Daria V. Rakitina Anastasia S. Nikitina Aleksandr I. Manolov Alina N. Egorova Eugene E. Kulikov Innokentii E. Vishnyakov Gleb Y. Fisunov Vadim M. Govorun Outer membrane vesicles secreted by pathogenic and nonpathogenic Bacteroides fragilis represent different metabolic activities
description	Abstract Numerous studies are devoted to the intestinal microbiota and intercellular communication maintaining homeostasis. In this regard, vesicles secreted by bacteria represent one of the most popular topics for research. For example, the outer membrane vesicles (OMVs) of Bacteroides fragilis play an important nutritional role with respect to other microorganisms and promote anti-inflammatory effects on immune cells. However, toxigenic B. fragilis (ETBF) contributes to bowel disease, even causing colon cancer. If nontoxigenic B. fragilis (NTBF) vesicles exert a beneficial effect on the intestine, it is likely that ETBF vesicles can be utilized for potential pathogenic implementation. To confirm this possibility, we performed comparative proteomic HPLC-MS/MS analysis of vesicles isolated from ETBF and NTBF. Furthermore, we performed, for the first time, HPLC-MS/MS and GS-MS comparative metabolomic analysis for the vesicles isolated from both strains with subsequent reconstruction of the vesicle metabolic pathways. We utilized fluxomic experiments to validate the reconstructed biochemical reaction activities and finally observed considerable difference in the vesicle proteome and metabolome profiles. Compared with NTBF OMVs, metabolic activity of ETBF OMVs provides their similarity to micro reactors that are likely to be used for long-term persistence and implementing pathogenic potential in the host.
format	article
author	Natalya B. Zakharzhevskaya Anna A. Vanyushkina Ilya A. Altukhov Aleksey L. Shavarda Ivan O. Butenko Daria V. Rakitina Anastasia S. Nikitina Aleksandr I. Manolov Alina N. Egorova Eugene E. Kulikov Innokentii E. Vishnyakov Gleb Y. Fisunov Vadim M. Govorun
author_facet	Natalya B. Zakharzhevskaya Anna A. Vanyushkina Ilya A. Altukhov Aleksey L. Shavarda Ivan O. Butenko Daria V. Rakitina Anastasia S. Nikitina Aleksandr I. Manolov Alina N. Egorova Eugene E. Kulikov Innokentii E. Vishnyakov Gleb Y. Fisunov Vadim M. Govorun
author_sort	Natalya B. Zakharzhevskaya
title	Outer membrane vesicles secreted by pathogenic and nonpathogenic Bacteroides fragilis represent different metabolic activities
title_short	Outer membrane vesicles secreted by pathogenic and nonpathogenic Bacteroides fragilis represent different metabolic activities
title_full	Outer membrane vesicles secreted by pathogenic and nonpathogenic Bacteroides fragilis represent different metabolic activities
title_fullStr	Outer membrane vesicles secreted by pathogenic and nonpathogenic Bacteroides fragilis represent different metabolic activities
title_full_unstemmed	Outer membrane vesicles secreted by pathogenic and nonpathogenic Bacteroides fragilis represent different metabolic activities
title_sort	outer membrane vesicles secreted by pathogenic and nonpathogenic bacteroides fragilis represent different metabolic activities
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0a3a1a392b52497794ce3934fcb2bbae
work_keys_str_mv	AT natalyabzakharzhevskaya outermembranevesiclessecretedbypathogenicandnonpathogenicbacteroidesfragilisrepresentdifferentmetabolicactivities AT annaavanyushkina outermembranevesiclessecretedbypathogenicandnonpathogenicbacteroidesfragilisrepresentdifferentmetabolicactivities AT ilyaaaltukhov outermembranevesiclessecretedbypathogenicandnonpathogenicbacteroidesfragilisrepresentdifferentmetabolicactivities AT alekseylshavarda outermembranevesiclessecretedbypathogenicandnonpathogenicbacteroidesfragilisrepresentdifferentmetabolicactivities AT ivanobutenko outermembranevesiclessecretedbypathogenicandnonpathogenicbacteroidesfragilisrepresentdifferentmetabolicactivities AT dariavrakitina outermembranevesiclessecretedbypathogenicandnonpathogenicbacteroidesfragilisrepresentdifferentmetabolicactivities AT anastasiasnikitina outermembranevesiclessecretedbypathogenicandnonpathogenicbacteroidesfragilisrepresentdifferentmetabolicactivities AT aleksandrimanolov outermembranevesiclessecretedbypathogenicandnonpathogenicbacteroidesfragilisrepresentdifferentmetabolicactivities AT alinanegorova outermembranevesiclessecretedbypathogenicandnonpathogenicbacteroidesfragilisrepresentdifferentmetabolicactivities AT eugeneekulikov outermembranevesiclessecretedbypathogenicandnonpathogenicbacteroidesfragilisrepresentdifferentmetabolicactivities AT innokentiievishnyakov outermembranevesiclessecretedbypathogenicandnonpathogenicbacteroidesfragilisrepresentdifferentmetabolicactivities AT glebyfisunov outermembranevesiclessecretedbypathogenicandnonpathogenicbacteroidesfragilisrepresentdifferentmetabolicactivities AT vadimmgovorun outermembranevesiclessecretedbypathogenicandnonpathogenicbacteroidesfragilisrepresentdifferentmetabolicactivities
_version_	1718394378230169600

Outer membrane vesicles secreted by pathogenic and nonpathogenic Bacteroides fragilis represent different metabolic activities

Ejemplares similares