Ketoprofen-loaded Eudragit electrospun nanofibers for the treatment of oral mucositis

Rana Ihab Reda,1 Ming Ming Wen,2 Amal Hassan El-Kamel1 1Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, 2Department of Pharmaceutics, Faculty of Pharmacy and Drug Manufacturing, Pharos University in Alexandria, Alexandria, Egypt Purpose: The purpose of this study was to fo...

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Autores principales:	Reda RI, Wen MM, El-Kamel AH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Nanofibers electrospinning Ketoprofen Eudragit Oral mucositis. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0a3e767e869f4a1f8608e60f13013b4d
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spelling	oai:doaj.org-article:0a3e767e869f4a1f8608e60f13013b4d2021-12-02T02:21:18ZKetoprofen-loaded Eudragit electrospun nanofibers for the treatment of oral mucositis1178-2013https://doaj.org/article/0a3e767e869f4a1f8608e60f13013b4d2017-03-01T00:00:00Zhttps://www.dovepress.com/ketoprofen-loaded-eudragit-electrospun-nanofibers-for-the-treatment-of-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Rana Ihab Reda,1 Ming Ming Wen,2 Amal Hassan El-Kamel1 1Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, 2Department of Pharmaceutics, Faculty of Pharmacy and Drug Manufacturing, Pharos University in Alexandria, Alexandria, Egypt Purpose: The purpose of this study was to formulate ketoprofen (KET)-loaded Eudragit L and Eudragit S nanofibers (NFs) by the electrospinning technique for buccal administration to treat oral mucositis as a safe alternative to orally administered KET, which causes gastrointestinal tract (GIT) side effects. Materials and methods: NFs were prepared by electrospinning using Eudragit L and Eudragit S. Several variables were evaluated to optimize NF formulation, such as polymer types and concentrations, applied voltage, flow rate and drug concentrations. Differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) and analyses of drug contents, hydration capacity, surface pH, drug release and ex vivo permeation were performed to evaluate the NFs. The selected formulation (F1) was evaluated in vivo on induced oral mucositis in rabbits. Results: SEM revealed that 20% polymer formed smooth and bead-free NFs. DSC results confirmed the amorphous nature of KET in the NFs. FTIR confirmed hydrogen bond formation between the drug and polymer, which stabilized the NFs. Both formulations (F1 and F2) had an acceptable surface pH. The drug loading was >90%. The amount of KET released from NF formulations was statistically significantly higher (P≤0.001) than that released from the corresponding solvent-casted films. The complete release of KET from F1 occurred within 2 hours. Ex vivo permeation study revealed that only a small fraction of drug permeated from F1, which was a better candidate than F2 for local buccal delivery. In vivo evaluation of F1 on oral mucositis induced in rabbits demonstrated that F1 reduced the clinical severity of mucositis in rabbits under the current experimental conditions. The attenuated clinical severity was accompanied by a marked reduction in inflammatory infiltrate and re-epithelization of the epithelial layer. Conclusion: Eudragit L100 nanofibers (EL-NF) loaded with KET (F1) suppressed the inflammatory response associated with mucositis, which confirmed the efficacy of local buccal delivery of KET-loaded EL-NF in treating oral mucositis. Keywords: nanofibers, electrospinning, ketoprofen, Eudragit, oral mucositisReda RIWen MMEl-Kamel AHDove Medical PressarticleNanofiberselectrospinningKetoprofenEudragitOral mucositis.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 2335-2351 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanofibers electrospinning Ketoprofen Eudragit Oral mucositis. Medicine (General) R5-920
spellingShingle	Nanofibers electrospinning Ketoprofen Eudragit Oral mucositis. Medicine (General) R5-920 Reda RI Wen MM El-Kamel AH Ketoprofen-loaded Eudragit electrospun nanofibers for the treatment of oral mucositis
description	Rana Ihab Reda,1 Ming Ming Wen,2 Amal Hassan El-Kamel1 1Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, 2Department of Pharmaceutics, Faculty of Pharmacy and Drug Manufacturing, Pharos University in Alexandria, Alexandria, Egypt Purpose: The purpose of this study was to formulate ketoprofen (KET)-loaded Eudragit L and Eudragit S nanofibers (NFs) by the electrospinning technique for buccal administration to treat oral mucositis as a safe alternative to orally administered KET, which causes gastrointestinal tract (GIT) side effects. Materials and methods: NFs were prepared by electrospinning using Eudragit L and Eudragit S. Several variables were evaluated to optimize NF formulation, such as polymer types and concentrations, applied voltage, flow rate and drug concentrations. Differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) and analyses of drug contents, hydration capacity, surface pH, drug release and ex vivo permeation were performed to evaluate the NFs. The selected formulation (F1) was evaluated in vivo on induced oral mucositis in rabbits. Results: SEM revealed that 20% polymer formed smooth and bead-free NFs. DSC results confirmed the amorphous nature of KET in the NFs. FTIR confirmed hydrogen bond formation between the drug and polymer, which stabilized the NFs. Both formulations (F1 and F2) had an acceptable surface pH. The drug loading was >90%. The amount of KET released from NF formulations was statistically significantly higher (P≤0.001) than that released from the corresponding solvent-casted films. The complete release of KET from F1 occurred within 2 hours. Ex vivo permeation study revealed that only a small fraction of drug permeated from F1, which was a better candidate than F2 for local buccal delivery. In vivo evaluation of F1 on oral mucositis induced in rabbits demonstrated that F1 reduced the clinical severity of mucositis in rabbits under the current experimental conditions. The attenuated clinical severity was accompanied by a marked reduction in inflammatory infiltrate and re-epithelization of the epithelial layer. Conclusion: Eudragit L100 nanofibers (EL-NF) loaded with KET (F1) suppressed the inflammatory response associated with mucositis, which confirmed the efficacy of local buccal delivery of KET-loaded EL-NF in treating oral mucositis. Keywords: nanofibers, electrospinning, ketoprofen, Eudragit, oral mucositis
format	article
author	Reda RI Wen MM El-Kamel AH
author_facet	Reda RI Wen MM El-Kamel AH
author_sort	Reda RI
title	Ketoprofen-loaded Eudragit electrospun nanofibers for the treatment of oral mucositis
title_short	Ketoprofen-loaded Eudragit electrospun nanofibers for the treatment of oral mucositis
title_full	Ketoprofen-loaded Eudragit electrospun nanofibers for the treatment of oral mucositis
title_fullStr	Ketoprofen-loaded Eudragit electrospun nanofibers for the treatment of oral mucositis
title_full_unstemmed	Ketoprofen-loaded Eudragit electrospun nanofibers for the treatment of oral mucositis
title_sort	ketoprofen-loaded eudragit electrospun nanofibers for the treatment of oral mucositis
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/0a3e767e869f4a1f8608e60f13013b4d
work_keys_str_mv	AT redari ketoprofenloadedeudragitelectrospunnanofibersforthetreatmentoforalmucositis AT wenmm ketoprofenloadedeudragitelectrospunnanofibersforthetreatmentoforalmucositis AT elkamelah ketoprofenloadedeudragitelectrospunnanofibersforthetreatmentoforalmucositis
_version_	1718402511601139712

Ketoprofen-loaded Eudragit electrospun nanofibers for the treatment of oral mucositis

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