Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex

The wide diversity of cortical inhibitory neuron types populating the cortex allows the assembly of diverse microcircuits and endows these circuits with different computational properties. Thus, characterizing neuronal diversity is fundamental to describe the building blocks of cortical microcircuit...

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Autores principales:	Maximiliano José Nigro, Hinako Kirikae, Kasper Kjelsberg, Rajeevkumar Raveendran Nair, Menno P. Witter
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	parvalbumin interneurons PV-IRES-Cre interneuron diversity perirhinal cortex mouse line Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/0a43790aedc94f309496a7d6e920de30
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spelling	oai:doaj.org-article:0a43790aedc94f309496a7d6e920de302021-11-08T06:47:31ZNot All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex1662-511010.3389/fncir.2021.781928https://doaj.org/article/0a43790aedc94f309496a7d6e920de302021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fncir.2021.781928/fullhttps://doaj.org/toc/1662-5110The wide diversity of cortical inhibitory neuron types populating the cortex allows the assembly of diverse microcircuits and endows these circuits with different computational properties. Thus, characterizing neuronal diversity is fundamental to describe the building blocks of cortical microcircuits and probe their function. To this purpose, the mouse has emerged as a powerful tool to genetically label and manipulate specific inhibitory cell-types in the mammalian brain. Among these cell-types, the parvalbumin-expressing interneuron type (PV-INs) is perhaps the most characterized. Several mouse lines have been generated to target PV-INs. Among these mouse lines, the PV-IRES-Cre lines is the most widely used and demonstrated a high specificity and efficiency in targeting PV-INs in different cortical areas. However, a characterization of the performance across cortical regions is still missing. Here we show that the PV-IRES-Cre mouse line labels only a fraction of PV immunoreactive neurons in perirhinal cortex and other association areas. Our results point to a yet uncharacterized diversity within the PV-INs and emphasize the need to characterize these tools in specific cortical areas.Maximiliano José NigroHinako KirikaeHinako KirikaeKasper KjelsbergRajeevkumar Raveendran NairMenno P. WitterFrontiers Media S.A.articleparvalbumin interneuronsPV-IRES-Creinterneuron diversityperirhinal cortexmouse lineNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Neural Circuits, Vol 15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	parvalbumin interneurons PV-IRES-Cre interneuron diversity perirhinal cortex mouse line Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	parvalbumin interneurons PV-IRES-Cre interneuron diversity perirhinal cortex mouse line Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Maximiliano José Nigro Hinako Kirikae Hinako Kirikae Kasper Kjelsberg Rajeevkumar Raveendran Nair Menno P. Witter Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex
description	The wide diversity of cortical inhibitory neuron types populating the cortex allows the assembly of diverse microcircuits and endows these circuits with different computational properties. Thus, characterizing neuronal diversity is fundamental to describe the building blocks of cortical microcircuits and probe their function. To this purpose, the mouse has emerged as a powerful tool to genetically label and manipulate specific inhibitory cell-types in the mammalian brain. Among these cell-types, the parvalbumin-expressing interneuron type (PV-INs) is perhaps the most characterized. Several mouse lines have been generated to target PV-INs. Among these mouse lines, the PV-IRES-Cre lines is the most widely used and demonstrated a high specificity and efficiency in targeting PV-INs in different cortical areas. However, a characterization of the performance across cortical regions is still missing. Here we show that the PV-IRES-Cre mouse line labels only a fraction of PV immunoreactive neurons in perirhinal cortex and other association areas. Our results point to a yet uncharacterized diversity within the PV-INs and emphasize the need to characterize these tools in specific cortical areas.
format	article
author	Maximiliano José Nigro Hinako Kirikae Hinako Kirikae Kasper Kjelsberg Rajeevkumar Raveendran Nair Menno P. Witter
author_facet	Maximiliano José Nigro Hinako Kirikae Hinako Kirikae Kasper Kjelsberg Rajeevkumar Raveendran Nair Menno P. Witter
author_sort	Maximiliano José Nigro
title	Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex
title_short	Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex
title_full	Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex
title_fullStr	Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex
title_full_unstemmed	Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex
title_sort	not all that is gold glitters: pv-ires-cre mouse line shows low efficiency of labeling of parvalbumin interneurons in the perirhinal cortex
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0a43790aedc94f309496a7d6e920de30
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Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex

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