Combination of HLA-DR on Mycobacterium tuberculosis-Specific Cells and Tuberculosis Antigen/Phytohemagglutinin Ratio for Discriminating Active Tuberculosis From Latent Tuberculosis Infection

BackgroundNovel approaches for tuberculosis (TB) diagnosis, especially for distinguishing active TB (ATB) from latent TB infection (LTBI), are urgently warranted. The present study aims to determine whether the combination of HLA-DR on Mycobacterium tuberculosis (MTB)-specific cells and TB antigen/p...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ying Luo, Ying Xue, Guoxing Tang, Qun Lin, Huijuan Song, Wei Liu, Botao Yin, Jin Huang, Wei Wei, Liyan Mao, Feng Wang, Ziyong Sun
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Mycobacterium tuberculosis-specific cells HLA-DR TBAg/PHA ratio discrimination active tuberculosis latent tuberculosis infection Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/0a4484b8df1a4f30b2d0abffb5421ef1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0a4484b8df1a4f30b2d0abffb5421ef1
record_format	dspace
spelling	oai:doaj.org-article:0a4484b8df1a4f30b2d0abffb5421ef12021-11-11T08:40:50ZCombination of HLA-DR on Mycobacterium tuberculosis-Specific Cells and Tuberculosis Antigen/Phytohemagglutinin Ratio for Discriminating Active Tuberculosis From Latent Tuberculosis Infection1664-322410.3389/fimmu.2021.761209https://doaj.org/article/0a4484b8df1a4f30b2d0abffb5421ef12021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.761209/fullhttps://doaj.org/toc/1664-3224BackgroundNovel approaches for tuberculosis (TB) diagnosis, especially for distinguishing active TB (ATB) from latent TB infection (LTBI), are urgently warranted. The present study aims to determine whether the combination of HLA-DR on Mycobacterium tuberculosis (MTB)-specific cells and TB antigen/phytohemagglutinin (TBAg/PHA) ratio could facilitate MTB infection status discrimination.MethodsBetween June 2020 and June 2021, participants with ATB and LTBI were recruited from Tongji Hospital (Qiaokou cohort) and Sino-French New City Hospital (Caidian cohort), respectively. The detection of HLA-DR on MTB-specific cells upon TB antigen stimulation and T-SPOT assay were simultaneously performed on all subjects.ResultsA total of 116 (54 ATB and 62 LTBI) and another 84 (43 ATB and 41 LTBI) cases were respectively enrolled from Qiaokou cohort and Caidian cohort. Both HLA-DR on IFN-γ+TNF-α+ cells and TBAg/PHA ratio showed discriminatory value in distinguishing between ATB and LTBI. Receiver operator characteristic (ROC) curve analysis showed that HLA-DR on IFN-γ+TNF-α+ cells produced an area under the ROC curve (AUC) of 0.886. Besides, TBAg/PHA ratio yield an AUC of 0.736. Furthermore, the combination of these two indicators resulted in the accurate discrimination with an AUC of 0.937. When the threshold was set as 0.36, the diagnostic model could differentiate ATB from LTBI with a sensitivity of 92.00% and a specificity of 81.82%. The performance obtained in Qiaokou cohort was further validated in Caidian cohort.ConclusionsThe combination of HLA-DR on MTB-specific cells and TBAg/PHA ratio could serve as a robust tool to determine TB disease states.Ying LuoYing XueGuoxing TangQun LinHuijuan SongWei LiuBotao YinJin HuangWei WeiLiyan MaoFeng WangZiyong SunFrontiers Media S.A.articleMycobacterium tuberculosis-specific cellsHLA-DRTBAg/PHA ratiodiscriminationactive tuberculosislatent tuberculosis infectionImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mycobacterium tuberculosis-specific cells HLA-DR TBAg/PHA ratio discrimination active tuberculosis latent tuberculosis infection Immunologic diseases. Allergy RC581-607
spellingShingle	Mycobacterium tuberculosis-specific cells HLA-DR TBAg/PHA ratio discrimination active tuberculosis latent tuberculosis infection Immunologic diseases. Allergy RC581-607 Ying Luo Ying Xue Guoxing Tang Qun Lin Huijuan Song Wei Liu Botao Yin Jin Huang Wei Wei Liyan Mao Feng Wang Ziyong Sun Combination of HLA-DR on Mycobacterium tuberculosis-Specific Cells and Tuberculosis Antigen/Phytohemagglutinin Ratio for Discriminating Active Tuberculosis From Latent Tuberculosis Infection
description	BackgroundNovel approaches for tuberculosis (TB) diagnosis, especially for distinguishing active TB (ATB) from latent TB infection (LTBI), are urgently warranted. The present study aims to determine whether the combination of HLA-DR on Mycobacterium tuberculosis (MTB)-specific cells and TB antigen/phytohemagglutinin (TBAg/PHA) ratio could facilitate MTB infection status discrimination.MethodsBetween June 2020 and June 2021, participants with ATB and LTBI were recruited from Tongji Hospital (Qiaokou cohort) and Sino-French New City Hospital (Caidian cohort), respectively. The detection of HLA-DR on MTB-specific cells upon TB antigen stimulation and T-SPOT assay were simultaneously performed on all subjects.ResultsA total of 116 (54 ATB and 62 LTBI) and another 84 (43 ATB and 41 LTBI) cases were respectively enrolled from Qiaokou cohort and Caidian cohort. Both HLA-DR on IFN-γ+TNF-α+ cells and TBAg/PHA ratio showed discriminatory value in distinguishing between ATB and LTBI. Receiver operator characteristic (ROC) curve analysis showed that HLA-DR on IFN-γ+TNF-α+ cells produced an area under the ROC curve (AUC) of 0.886. Besides, TBAg/PHA ratio yield an AUC of 0.736. Furthermore, the combination of these two indicators resulted in the accurate discrimination with an AUC of 0.937. When the threshold was set as 0.36, the diagnostic model could differentiate ATB from LTBI with a sensitivity of 92.00% and a specificity of 81.82%. The performance obtained in Qiaokou cohort was further validated in Caidian cohort.ConclusionsThe combination of HLA-DR on MTB-specific cells and TBAg/PHA ratio could serve as a robust tool to determine TB disease states.
format	article
author	Ying Luo Ying Xue Guoxing Tang Qun Lin Huijuan Song Wei Liu Botao Yin Jin Huang Wei Wei Liyan Mao Feng Wang Ziyong Sun
author_facet	Ying Luo Ying Xue Guoxing Tang Qun Lin Huijuan Song Wei Liu Botao Yin Jin Huang Wei Wei Liyan Mao Feng Wang Ziyong Sun
author_sort	Ying Luo
title	Combination of HLA-DR on Mycobacterium tuberculosis-Specific Cells and Tuberculosis Antigen/Phytohemagglutinin Ratio for Discriminating Active Tuberculosis From Latent Tuberculosis Infection
title_short	Combination of HLA-DR on Mycobacterium tuberculosis-Specific Cells and Tuberculosis Antigen/Phytohemagglutinin Ratio for Discriminating Active Tuberculosis From Latent Tuberculosis Infection
title_full	Combination of HLA-DR on Mycobacterium tuberculosis-Specific Cells and Tuberculosis Antigen/Phytohemagglutinin Ratio for Discriminating Active Tuberculosis From Latent Tuberculosis Infection
title_fullStr	Combination of HLA-DR on Mycobacterium tuberculosis-Specific Cells and Tuberculosis Antigen/Phytohemagglutinin Ratio for Discriminating Active Tuberculosis From Latent Tuberculosis Infection
title_full_unstemmed	Combination of HLA-DR on Mycobacterium tuberculosis-Specific Cells and Tuberculosis Antigen/Phytohemagglutinin Ratio for Discriminating Active Tuberculosis From Latent Tuberculosis Infection
title_sort	combination of hla-dr on mycobacterium tuberculosis-specific cells and tuberculosis antigen/phytohemagglutinin ratio for discriminating active tuberculosis from latent tuberculosis infection
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0a4484b8df1a4f30b2d0abffb5421ef1
work_keys_str_mv	AT yingluo combinationofhladronmycobacteriumtuberculosisspecificcellsandtuberculosisantigenphytohemagglutininratiofordiscriminatingactivetuberculosisfromlatenttuberculosisinfection AT yingxue combinationofhladronmycobacteriumtuberculosisspecificcellsandtuberculosisantigenphytohemagglutininratiofordiscriminatingactivetuberculosisfromlatenttuberculosisinfection AT guoxingtang combinationofhladronmycobacteriumtuberculosisspecificcellsandtuberculosisantigenphytohemagglutininratiofordiscriminatingactivetuberculosisfromlatenttuberculosisinfection AT qunlin combinationofhladronmycobacteriumtuberculosisspecificcellsandtuberculosisantigenphytohemagglutininratiofordiscriminatingactivetuberculosisfromlatenttuberculosisinfection AT huijuansong combinationofhladronmycobacteriumtuberculosisspecificcellsandtuberculosisantigenphytohemagglutininratiofordiscriminatingactivetuberculosisfromlatenttuberculosisinfection AT weiliu combinationofhladronmycobacteriumtuberculosisspecificcellsandtuberculosisantigenphytohemagglutininratiofordiscriminatingactivetuberculosisfromlatenttuberculosisinfection AT botaoyin combinationofhladronmycobacteriumtuberculosisspecificcellsandtuberculosisantigenphytohemagglutininratiofordiscriminatingactivetuberculosisfromlatenttuberculosisinfection AT jinhuang combinationofhladronmycobacteriumtuberculosisspecificcellsandtuberculosisantigenphytohemagglutininratiofordiscriminatingactivetuberculosisfromlatenttuberculosisinfection AT weiwei combinationofhladronmycobacteriumtuberculosisspecificcellsandtuberculosisantigenphytohemagglutininratiofordiscriminatingactivetuberculosisfromlatenttuberculosisinfection AT liyanmao combinationofhladronmycobacteriumtuberculosisspecificcellsandtuberculosisantigenphytohemagglutininratiofordiscriminatingactivetuberculosisfromlatenttuberculosisinfection AT fengwang combinationofhladronmycobacteriumtuberculosisspecificcellsandtuberculosisantigenphytohemagglutininratiofordiscriminatingactivetuberculosisfromlatenttuberculosisinfection AT ziyongsun combinationofhladronmycobacteriumtuberculosisspecificcellsandtuberculosisantigenphytohemagglutininratiofordiscriminatingactivetuberculosisfromlatenttuberculosisinfection
_version_	1718439281600495616

Combination of HLA-DR on Mycobacterium tuberculosis-Specific Cells and Tuberculosis Antigen/Phytohemagglutinin Ratio for Discriminating Active Tuberculosis From Latent Tuberculosis Infection

Ejemplares similares